Data/hora: 24/06/2024 19:23:14

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 08/04/2016
Data da última atualização: | 29/12/2017
Autoria: | JIMENEZ, C. R.; PENITENTE-FILHO, J. M.; TORRES, C. A. A.; MADEIROS, A. M.; SILVA, L. S.
Afiliação: | CAROLINA R. JIMENEZ, USP; JURANDY M. PENITENTE-FILHO, UFV; CIRO A. A. TORRES, UFV; AMANDA M. MEDEIROS, UFV; LEANDRO S. SILVA, UFV.
Título: | Vitrification of bovine preantral follicles with dimenthylsulfoxide and sucrose plus a-tocpherol.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 3, p. 209-215, mar. 2016.
Idioma: | Inglês
Conteúdo: | The objective of this study was to evaluate the vitrification of bovine preantral follicles with dimethylsulfoxide (D) and sucrose (S) plus &#945;-tocopherol 5mmol/L (T5) or 10mmol/L (T10) and, evaluate the thawed with minimal essential medium (m) with or without sucrose (s). Ovaries of cows were collected from slaughterhouse for the experiment I (n=66) and II (n=51). In the laboratory ovarian fragments were randomly assigned either to fresh control and 8 vitrification treatments (Controle and Dm; Dms, DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Ovarian fragments were placed in vitrification solution (5 min) and immersed in liquid nitrogen (-196°C), after a week, the fragments were thawed and analyzed. In the experiments I, preantral follicles were morphologically observed for histological evaluation, (normal; degenerated and developing of stage). In the experiment II, preantral follicles were mechanically isolated from ovarian tissue and examined with trypan blue, where dead and live corresponded to stained or non-stained. The treatments DSm, DSms and DST10m were effective in preserving the morphology in situ. However, the viability of isolated preantral follicles after vitrification remained high only in treatment DST10m. Thus, DST10m preserves survival rates and morphological integrity during vitrification of bovine preantral follicles.
Palavras-Chave: | Células granulosas; Crioprotetor; Dimenthylsulfoxide; Dimetilsulfóxido; Oócito.
Thesagro: | Antioxidante; Criopreservação.
Thesaurus Nal: | antioxidants; Cryopreservation; Granulosa cells; Oocytes.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/142197/1/Vitrificantion-of-bovine.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59302-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS