Data/hora: 06/06/2024 06:36:50

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	06/02/2017	
Data da última atualização: 	11/10/2017	
Autoria: 	ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A.; JESUS, C. C.; CALEGARIO, R. F.; KITAJIMA, E. W.; NOVELLI, V. M.; ASTUA, J. de F.	
Afiliação: 	GABRIELLA DIAS ARENA; PEDRO LUIS RAMOS-GONZÁLEZ; MARIA ANDRÉIA NUNES; CAMILA CHABI JESUS; RENATA FAIER CALEGARIO; ELLIOT WATANABE KITAJIMA; VALDENICE MOREIRA NOVELLI; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Scientia Agricola, v.74, n.1, p.1-10X, 2017	
ISSN: 	1678-992X	
DOI: 	10.1590/1678-992X-2015-0380	
Idioma: 	Inglês	
Conteúdo: 	Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum violaefolium ringspot virus, Coffee ringspot virus, Dichorhavirus	
Thesagro: 	Doença de planta; Virus.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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