Data/hora: 03/06/2024 15:52:39

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	13/09/2022	
Data da última atualização: 	13/09/2022	
Autoria: 	CARVALHEIRA, L. de R.; SANTOS, G. B. dos; JASMIM; GUIMARÃES, C. R.; CAMPOS, M. M.; MACHADO, F. S.; PEDROSO, A. M.; SILVA, T. E. da; NOGUEIRA, L. A. G.; RODRIGUES, A. L. R.; CARVALHO, B. C. de.	
Afiliação: 	LUCIANO DE REZENDE CARVALHEIRA, Universidade Federal Fluminense; GUSTAVO BERVIAN DOS SANTOS, Universidade Federal Fluminense; JASMIM, Universidade Federal do Rio de Janeiro; CLÓVIS RIBEIRO GUIMARÃES, Universidade Federal de Minas Gerais; MARIANA MAGALHAES CAMPOS, CNPGL; FERNANDA SAMARINI MACHADO, CNPGL; ALEXANDRE MENDONÇA PEDROSO, AMP Consultoria; TADEU EDER DA SILVA, University of Wisconsin-Madison; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; ANDRÉ LUÍS RIOS RODRIGUES, Universidade Federal Fluminense; BRUNO CAMPOS DE CARVALHO, CNPGL.	
Título: 	Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Animal Reproduction, v. 19, n. 3, e20210088, 2022.	
DOI: 	https://doi.org/10.1590/1984-3143-AR2021-0088	
Idioma: 	Inglês	
Conteúdo: 	This study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumenundegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes? DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes? DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes-embryo development impacting fertility of lactating dairy cows.	
Palavras-Chave: 	Apoptose.	
Thesagro: 	Bovino; Gado Leiteiro; Nutrição Animal; Reprodução Animal; Uréia.	
Thesaurus Nal: 	Animal nutrition; Animal reproduction; Apoptosis; Urea.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146377/1/Diet-crude-protein-reduction-on-follicular-fluid.pdf	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25760-1) - UPC - AP - DD -  -  -  - DIS