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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
29/12/2014 |
Data da última atualização: |
30/12/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. |
Afiliação: |
CNPSO - estagiário; UENP; CNPSO - estagiário; UENP; CNPSO - estagiário; CNPSO - estagiário; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO. |
Título: |
Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014. |
Idioma: |
Inglês |
Conteúdo: |
Phakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. MenosPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. T... Mostrar Tudo |
Palavras-Chave: |
Ferrugem asiática da soja. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114606/1/Temporal-gene-expression-analysis-of-effector-candidates-of-Phakopsora-pachyrhizi-across-the-infection-cycle.pdf
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Marc: |
LEADER 02492nam a2200193 a 4500 001 2003884 005 2014-12-30 008 2014 bl uuuu u00u1 u #d 100 1 $aPOLIZEL-PODANOSQUI, A. M. 245 $aTemporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle.$h[electronic resource] 260 $aIn: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions$c2014 520 $aPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. 653 $aFerrugem asiática da soja 700 1 $aCARVALHO, M. C. C. G. de 700 1 $aROCHA, C. S. 700 1 $aDARBEN, L. M. 700 1 $aLOPES-CAITAR, V. S. 700 1 $aAOYAOGI, L. N. 700 1 $aMARCELINO-GUIMARÃES, F. C.
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Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
03/12/2013 |
Data da última atualização: |
20/10/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RANDOW, C. von; ZERI, M.; RESTREPO-COUPE, N.; MUZA, M. N.; GONÇALVES, L. G. G. de; COSTA, M. H.; ARAUJO, A. C.; MANZI, A. O.; ROCHA, H. R. da; SALESKA, S. R.; ARAIN, M. A.; BAKER, I. T.; CESTARO, B. P.; CHRISTOFFERSEN, B.; CIAIS, P.; FISHER, J. B.; GALBRAITH, D.; GUAN, X.; HURK, B. van den; ICHII, K.; IMBUZEIRO, H.; JAIN, A.; LEVINE, N.; MIGUEZ-MACHO, G.; POULTER, B.; ROBERTI, D. R.; SAHOO, A.; SCHAEFER, K.; SHI, M.; TIAN, H.; VERBEECK, H.; YANG, Z.-L. |
Afiliação: |
CELSO VON RANDOW, INPE; MARCELO ZERI, INPE; NATALIA RESTREPO-COUPE, University of Technology; MICHEL N. MUZA, Instituto Federal de Santa Catarina; LUIS GUSTAVO G. DE GONÇALVES, INPE; MARCOS H. COSTA, UFV; ALESSANDRO CARIOCA DE ARAUJO, CPATU; ANTONIO O. MANZI, INPA; HUMBERTO R. DA ROCHA, USP; SCOTT R. SALESKA, UNIVERSITY OF ARIZONA; M. ALAF ARAIN, McMaster University; IAN T. BAKER, COLORADO STATE UNIVERSITY; BRUNO P. CESTARO, USP; BRADLEY CHRISTOFFERSEN, University of Arizona; PHILIPPE CIAIS, CEA Orme des Merisiers; JOSHUA B. FISHER, California Institute of Technology; DAVID GALBRAITH, UNIVERSITY OF LEEDS; XIAODAN GUAN, The University of Texas at Austin; BART VAN DEN HURK, Royal Netherlands Meteorological Institute; KAZUHITO ICHII, Fukushima University; HEWLLEY IMBUZEIRO, UFV; ATUL JAIN, University of Illinois at Urbana-Champaign; NAOMI LEVINE, HARVARD UNIVERSITY; GONZALO MIGUEZ-MACHO, University of Santiago de Compostela; BEN POULTER, CEA Orme des Merisiers; DEBORA R. ROBERTI, UFSM; ALOK SAHOO, Princeton University; KEVIN SCHAEFER, University of Colorado at Boulder; MINGJIE SHI, The University of Texas at Austin; HANQIN TIAN, Auburn University; HANS VERBEECK, Ghent University; ZONG-LIANG YANG, The University of Texas at Austin. |
Título: |
Inter-annual variability of carbon and water fluxes in Amazonian forest, Cerrado and pasture sites, as simulated by terrestrial biosphere models. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Agricultural and Forest Meteorology, v. 182-183, p. 144-155, Dec. 2013. |
DOI: |
http://dx.doi.org/10.1016/j.agrformet.2013.05.015 |
Idioma: |
Inglês |
Conteúdo: |
This study analyzes the inter-annual variability (IAV) of simulations of 21 different land surface model formulations, driven by meteorological conditions measured at 8 flux towers, located in rain forest, forest-savanna ecotone and pasture sites in Amazonia, and one in savanna site in Southeastern Brazil. Annual totals of net ecosystem exchange (NEE) of carbon and evapotranspiration (ET), measured and simulated by each model for each site-year, were compared in terms of year-to-year variability and possible relation to climate drivers. Results have shown that most of models simulations for annual totals of NEE and ET, and IAV of these fluxes, are frequently different from measurements. The average of the model simulations of annual fluxes tend to respond to climatic drivers similarly to the observations, but with noticeable discrepancies. Annual measurements of NEE are negatively correlated to annual rainfall in the forest sites group. Although the ensemble of all models yields a similar result, only three model formulations reproduce a significant negative correlation of simulated NEE with rainfall. For the IAV of ET, tower measurements are controlled by annual variations of radiation and this feature is captured by the ensemble of the models, both at individual sites and when all forest sites are grouped. However, simulated ET values are also significantly correlated to the amount of precipitation in many models and in the model ensemble, while there is no significant correlation in the observations. In general, the surface models are able to reproduce the responses of fluxes to climatic drivers, but improvements are still needed to better capture their inter-annual variability. MenosThis study analyzes the inter-annual variability (IAV) of simulations of 21 different land surface model formulations, driven by meteorological conditions measured at 8 flux towers, located in rain forest, forest-savanna ecotone and pasture sites in Amazonia, and one in savanna site in Southeastern Brazil. Annual totals of net ecosystem exchange (NEE) of carbon and evapotranspiration (ET), measured and simulated by each model for each site-year, were compared in terms of year-to-year variability and possible relation to climate drivers. Results have shown that most of models simulations for annual totals of NEE and ET, and IAV of these fluxes, are frequently different from measurements. The average of the model simulations of annual fluxes tend to respond to climatic drivers similarly to the observations, but with noticeable discrepancies. Annual measurements of NEE are negatively correlated to annual rainfall in the forest sites group. Although the ensemble of all models yields a similar result, only three model formulations reproduce a significant negative correlation of simulated NEE with rainfall. For the IAV of ET, tower measurements are controlled by annual variations of radiation and this feature is captured by the ensemble of the models, both at individual sites and when all forest sites are grouped. However, simulated ET values are also significantly correlated to the amount of precipitation in many models and in the model ensemble, while there is no significant cor... Mostrar Tudo |
Palavras-Chave: |
Fluxo de carbono; Modelagem. |
Thesagro: |
Cerrado; Floresta; Pastagem. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 03311naa a2200577 a 4500 001 1972830 005 2022-10-20 008 2013 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1016/j.agrformet.2013.05.015$2DOI 100 1 $aRANDOW, C. von 245 $aInter-annual variability of carbon and water fluxes in Amazonian forest, Cerrado and pasture sites, as simulated by terrestrial biosphere models.$h[electronic resource] 260 $c2013 520 $aThis study analyzes the inter-annual variability (IAV) of simulations of 21 different land surface model formulations, driven by meteorological conditions measured at 8 flux towers, located in rain forest, forest-savanna ecotone and pasture sites in Amazonia, and one in savanna site in Southeastern Brazil. Annual totals of net ecosystem exchange (NEE) of carbon and evapotranspiration (ET), measured and simulated by each model for each site-year, were compared in terms of year-to-year variability and possible relation to climate drivers. Results have shown that most of models simulations for annual totals of NEE and ET, and IAV of these fluxes, are frequently different from measurements. The average of the model simulations of annual fluxes tend to respond to climatic drivers similarly to the observations, but with noticeable discrepancies. Annual measurements of NEE are negatively correlated to annual rainfall in the forest sites group. Although the ensemble of all models yields a similar result, only three model formulations reproduce a significant negative correlation of simulated NEE with rainfall. For the IAV of ET, tower measurements are controlled by annual variations of radiation and this feature is captured by the ensemble of the models, both at individual sites and when all forest sites are grouped. However, simulated ET values are also significantly correlated to the amount of precipitation in many models and in the model ensemble, while there is no significant correlation in the observations. In general, the surface models are able to reproduce the responses of fluxes to climatic drivers, but improvements are still needed to better capture their inter-annual variability. 650 $aAmazonia 650 $aCerrado 650 $aFloresta 650 $aPastagem 653 $aFluxo de carbono 653 $aModelagem 700 1 $aZERI, M. 700 1 $aRESTREPO-COUPE, N. 700 1 $aMUZA, M. N. 700 1 $aGONÇALVES, L. G. G. de 700 1 $aCOSTA, M. H. 700 1 $aARAUJO, A. C. 700 1 $aMANZI, A. O. 700 1 $aROCHA, H. R. da 700 1 $aSALESKA, S. R. 700 1 $aARAIN, M. A. 700 1 $aBAKER, I. T. 700 1 $aCESTARO, B. P. 700 1 $aCHRISTOFFERSEN, B. 700 1 $aCIAIS, P. 700 1 $aFISHER, J. B. 700 1 $aGALBRAITH, D. 700 1 $aGUAN, X. 700 1 $aHURK, B. van den 700 1 $aICHII, K. 700 1 $aIMBUZEIRO, H. 700 1 $aJAIN, A. 700 1 $aLEVINE, N. 700 1 $aMIGUEZ-MACHO, G. 700 1 $aPOULTER, B. 700 1 $aROBERTI, D. R. 700 1 $aSAHOO, A. 700 1 $aSCHAEFER, K. 700 1 $aSHI, M. 700 1 $aTIAN, H. 700 1 $aVERBEECK, H. 700 1 $aYANG, Z.-L. 773 $tAgricultural and Forest Meteorology$gv. 182-183, p. 144-155, Dec. 2013.
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