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Registros recuperados : 11 | |
3. | | MASCARIN, G. M.; KOBORI, N. N.; JACKSON, M. A.; DUNLAP, C. A.; DELALIBERA JUNIOR, I. Nitrogen sources affect productivity, desiccation tolerance and storage stability of Beauveria bassiana blastospores. Journal of Applied Microbiology, v. 124, n. 3, p. 810-820, 2018. Biblioteca(s): Embrapa Meio Ambiente. |
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4. | | MASCARIN, G. M.; KOBORI, N. N.; COLEMAN, J. J.; JACKSON, M. A. Impact of osmotic stress on production, morphology, and fitness of Beauveria bassiana blastospores. Applied Microbiology and Biotechnology, v. 107, n. 15, p. 4815-4831, 2023. Biblioteca(s): Embrapa Meio Ambiente. |
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5. | | MASCARIN, G. M.; JACKSON, M. A.; BEHLE, R. W.; KOBORI, N. N.; DELALIBERA JÚNIOR, I. Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes. Applied Microbiology and Biotechnology, Berlin, v. 100, n. 19, p. 8359-8370, Oct. 2016. Published online: 20 May 2016. Biblioteca(s): Embrapa Arroz e Feijão. |
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9. | | MASCARIN, G. M.; JACKSON, M. A.; KOBORI, N. N.; BEHLE, R. W.; DUNLAP, C. A.; DELALIBERA JÚNIOR, I. Glucose concentration alters dissolved oxygen levels in liquid cultures of Beauveria bassiana and affects formation and bioefficacy of blastospores. Applied Microbiology and Biotechnology, Berlin, v. 99, n. 16, p. 6653-6665, Aug. 2015. Publicado online: 07 May 2015. Biblioteca(s): Embrapa Arroz e Feijão. |
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10. | | MORALES-REYES, C.; MASCARIN, G. M.; JACKSON, M. A.; HALL, D.; SANCHEZ-PENA, S. R.; ARTHURS, S. P. Comparison of aerial conidia and blastospores from two entomopathogenic fungi against Diaphorina citri (Hemiptera: Liviidae) under laboratory and greenhouse conditions. Biocontrol Science and Technology, v. 28, n. 8, p. 737-749, 2018. Biblioteca(s): Embrapa Meio Ambiente. |
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11. | | MASCARIN, G. M.; KOBORI, N. N.; VITAL, R. C. de J.; JACKSON, M. A.; QUINTELA, E. D. Production of microsclerotia by Brazilian strains of Metarhizium spp. using submerged liquid culture fermentation. World Journal of Microbiology & Biotechnology, Oxford, v. 30, n. 5, p. 1583-1590, May 2014. Biblioteca(s): Embrapa Arroz e Feijão. |
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Registros recuperados : 11 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
26/09/2017 |
Data da última atualização: |
18/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
DUNLAP, C. A.; MASCARIN, G. M.; ROMAGNOLI, E. M.; JACKSON, M. A. |
Afiliação: |
CHRISTOPHER A. DUNLAP, USDA, Peoria-IL; GABRIEL MOURA MASCARIN, CNPAF; EMILIANA M. ROMAGNOLI, USDA, Peoria-IL; MARK A. JACKSON, USDA, Peoria-IL. |
Título: |
Rapid discrimination of Isaria javanica and Isaria poprawskii from Isaria spp. using high resolution DNA melting assays. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of Invertebrate Pathology, v. 150, p. 88-93, 2017. |
ISSN: |
0022-2011 |
DOI: |
10.1016/j.jip.2017.09.011 |
Idioma: |
Inglês |
Conteúdo: |
The current study evaluates the potential of using high resolution DNA melting assays to discriminate species in the genus Isaria. The study utilizes a previously identified 103 base pair PCR amplicon, which was reported to be selective for Isaria fumosorosea. Our study finds the amplicon selective for Isaria javanica and Isaria poprawskii when assayed against all members of the genus. In addition, the high resolution melting profile of this amplicon can be used to discriminate between I. javanica, I. poprawskii and a 1:1 mixture of the two species. The practical application of this technique was confirmed using a bioassay on whitefly nymphs (Bemisia tabaci biotype B) inoculated with I. javanica, I. poprawskii or a 1:1 mixture of the two species. This assay provides a simple assay to identify these two species of entomopathogenic fungi. |
Palavras-Chave: |
Isaria javanica; Isaria poprawskii; SCAR marker. |
Thesagro: |
Controle biológico; Taxonomia. |
Thesaurus NAL: |
Biological control; Cordycipitaceae; Taxonomy. |
Categoria do assunto: |
O Insetos e Entomologia |
Marc: |
LEADER 01690naa a2200277 a 4500 001 2076265 005 2024-04-18 008 2017 bl uuuu u00u1 u #d 022 $a0022-2011 024 7 $a10.1016/j.jip.2017.09.011$2DOI 100 1 $aDUNLAP, C. A. 245 $aRapid discrimination of Isaria javanica and Isaria poprawskii from Isaria spp. using high resolution DNA melting assays.$h[electronic resource] 260 $c2017 520 $aThe current study evaluates the potential of using high resolution DNA melting assays to discriminate species in the genus Isaria. The study utilizes a previously identified 103 base pair PCR amplicon, which was reported to be selective for Isaria fumosorosea. Our study finds the amplicon selective for Isaria javanica and Isaria poprawskii when assayed against all members of the genus. In addition, the high resolution melting profile of this amplicon can be used to discriminate between I. javanica, I. poprawskii and a 1:1 mixture of the two species. The practical application of this technique was confirmed using a bioassay on whitefly nymphs (Bemisia tabaci biotype B) inoculated with I. javanica, I. poprawskii or a 1:1 mixture of the two species. This assay provides a simple assay to identify these two species of entomopathogenic fungi. 650 $aBiological control 650 $aCordycipitaceae 650 $aTaxonomy 650 $aControle biológico 650 $aTaxonomia 653 $aIsaria javanica 653 $aIsaria poprawskii 653 $aSCAR marker 700 1 $aMASCARIN, G. M. 700 1 $aROMAGNOLI, E. M. 700 1 $aJACKSON, M. A. 773 $tJournal of Invertebrate Pathology$gv. 150, p. 88-93, 2017.
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