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| Registros recuperados : 11 | |
| 3. |  | MASCARIN, G. M.; KOBORI, N. N.; JACKSON, M. A.; DUNLAP, C. A.; DELALIBERA JUNIOR, I. Nitrogen sources affect productivity, desiccation tolerance and storage stability of Beauveria bassiana blastospores. Journal of Applied Microbiology, v. 124, n. 3, p. 810-820, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 4. | | MASCARIN, G. M.; KOBORI, N. N.; COLEMAN, J. J.; JACKSON, M. A. Impact of osmotic stress on production, morphology, and fitness of Beauveria bassiana blastospores. Applied Microbiology and Biotechnology, v. 107, n. 15, p. 4815-4831, 2023.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 5. | | MASCARIN, G. M.; JACKSON, M. A.; BEHLE, R. W.; KOBORI, N. N.; DELALIBERA JÚNIOR, I. Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes. Applied Microbiology and Biotechnology, Berlin, v. 100, n. 19, p. 8359-8370, Oct. 2016. Published online: 20 May 2016.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 9. | | MASCARIN, G. M.; JACKSON, M. A.; KOBORI, N. N.; BEHLE, R. W.; DUNLAP, C. A.; DELALIBERA JÚNIOR, I. Glucose concentration alters dissolved oxygen levels in liquid cultures of Beauveria bassiana and affects formation and bioefficacy of blastospores. Applied Microbiology and Biotechnology, Berlin, v. 99, n. 16, p. 6653-6665, Aug. 2015. Publicado online: 07 May 2015.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | MORALES-REYES, C.; MASCARIN, G. M.; JACKSON, M. A.; HALL, D.; SANCHEZ-PENA, S. R.; ARTHURS, S. P. Comparison of aerial conidia and blastospores from two entomopathogenic fungi against Diaphorina citri (Hemiptera: Liviidae) under laboratory and greenhouse conditions. Biocontrol Science and Technology, v. 28, n. 8, p. 737-749, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | MASCARIN, G. M.; KOBORI, N. N.; VITAL, R. C. de J.; JACKSON, M. A.; QUINTELA, E. D. Production of microsclerotia by Brazilian strains of Metarhizium spp. using submerged liquid culture fermentation. World Journal of Microbiology & Biotechnology, Oxford, v. 30, n. 5, p. 1583-1590, May 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 11 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/03/2015 |
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Data da última atualização: |
11/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MASCARIN, G. M.; JACKSON, M. A.; KOBORI, N. N.; BEHLE, R. W.; DELALIBERA JÚNIOR, I. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPAF; MARK ALAN JACKSON, USDA; NILCE NAOMI KOBORI, USDA; ROBERT WARREN BEHLE, USDA; ITALO DELALIBERA JUNIOR, ESALQ. |
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Título: |
Liquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Invertebrate Pathology, v. 127, p. 11-20, 2015. |
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ISSN: |
0022-2011 |
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DOI: |
10.1016/j.jip.2014.12.001 |
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Idioma: |
Inglês |
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Conteúdo: |
A major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. Previous attempts in producing Beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. In this study, isolates of Beauveria bassiana and Isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and biocontrol efficacy using fermentation media containing acid hydrolyzed casein or cottonseed flour as the nitrogen source. Cultures of B. bassiana and I. fumosorosea grown in media containing cottonseed flour produced high blastospore concentrations (>1×109mL-1) after 3days which is comparatively less expensive nitrogen source than acid hydrolyzed casein. The resultant air-dried blastospores (<3% moisture) of all fungal isolates survived drying (61-86% viability), irrespective of the nitrogen source tested. Storage stability at 4°C varied with nitrogen source and fungal strain. Air-dried blastospores of B. bassiana strains showed half-lives >14months in contrast to 9.2-13.1months for I. fumosorosea. Blastospores of B. bassiana and I. fumosorosea killed Bemisia tabaci whitefly nymphs faster and required lower concentrations compared with aerial conidia. Our findings support the use of liquid culture fermentation as a cost-effective process to rapidly produce high yields of stable and infective blastospores of either B. bassiana or I. fumosorosea. These results support further evaluation of blastospore sprayable formulations for the control of soft-bodied insects. MenosA major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. Previous attempts in producing Beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. In this study, isolates of Beauveria bassiana and Isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and biocontrol efficacy using fermentation media containing acid hydrolyzed casein or cottonseed flour as the nitrogen source. Cultures of B. bassiana and I. fumosorosea grown in media containing cottonseed flour produced high blastospore concentrations (>1×109mL-1) after 3days which is comparatively less expensive nitrogen source than acid hydrolyzed casein. The resultant air-dried blastospores (<3% moisture) of all fungal isolates survived drying (61-86% viability), irrespective of the nitrogen source tested. Storage stability at 4°C varied with nitrogen source and fungal strain. Air-dried blastospores of B. bassiana strains showed half-lives >14months in contrast to 9.2-13.1months for I. fumosorosea. Blastospores of B. bassiana and I. fumosorosea killed Bemisia tabaci whitefly nymphs faster and required lower concentrations compared with aerial conidia. Our findings support the use of liquid culture fermentation as a cost-effective process to rapidly produce high yields of stable and i... Mostrar Tudo |
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Thesagro: |
Beauveria bassiana; Controle biológico; Fungo. |
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Thesaurus NAL: |
Bemisia argentifolii; Biological control agents; Isaria fumosorosea. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02543naa a2200265 a 4500
001 2011371
005 2016-03-11
008 2015 bl uuuu u00u1 u #d
022 $a0022-2011
024 7 $a10.1016/j.jip.2014.12.001$2DOI
100 1 $aMASCARIN, G. M.
245 $aLiquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains.$h[electronic resource]
260 $c2015
520 $aA major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. Previous attempts in producing Beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. In this study, isolates of Beauveria bassiana and Isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and biocontrol efficacy using fermentation media containing acid hydrolyzed casein or cottonseed flour as the nitrogen source. Cultures of B. bassiana and I. fumosorosea grown in media containing cottonseed flour produced high blastospore concentrations (>1×109mL-1) after 3days which is comparatively less expensive nitrogen source than acid hydrolyzed casein. The resultant air-dried blastospores (<3% moisture) of all fungal isolates survived drying (61-86% viability), irrespective of the nitrogen source tested. Storage stability at 4°C varied with nitrogen source and fungal strain. Air-dried blastospores of B. bassiana strains showed half-lives >14months in contrast to 9.2-13.1months for I. fumosorosea. Blastospores of B. bassiana and I. fumosorosea killed Bemisia tabaci whitefly nymphs faster and required lower concentrations compared with aerial conidia. Our findings support the use of liquid culture fermentation as a cost-effective process to rapidly produce high yields of stable and infective blastospores of either B. bassiana or I. fumosorosea. These results support further evaluation of blastospore sprayable formulations for the control of soft-bodied insects.
650 $aBemisia argentifolii
650 $aBiological control agents
650 $aIsaria fumosorosea
650 $aBeauveria bassiana
650 $aControle biológico
650 $aFungo
700 1 $aJACKSON, M. A.
700 1 $aKOBORI, N. N.
700 1 $aBEHLE, R. W.
700 1 $aDELALIBERA JÚNIOR, I.
773 $tJournal of Invertebrate Pathology$gv. 127, p. 11-20, 2015.
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