| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pantanal. |
|
Data corrente: |
27/11/2015 |
|
Data da última atualização: |
17/03/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; CABRAL, E. C.; COELHO, M. B.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. |
|
Afiliação: |
UNESP; UNESP; UNICAMP; UNICAMP; UNICAMP; UNICAMP; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP. |
|
Título: |
Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Theriogenology, v. 84, n. 1, p. 127-136, 2015. |
|
DOI: |
10.1016/j.theriogenology.2015.02.023 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species. MenosThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on... Mostrar Tudo |
|
Palavras-Chave: |
Animal embryos; Bovine embryo; Cryosurvival; In vitro production; Lipid profile. |
|
Thesagro: |
Bovino; Embrião animal; Reprodução animal. |
|
Thesaurus Nal: |
Cattle; conjugated linoleic acid; In vitro fertilization; mass spectrometry. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02882naa a2200373 a 4500
001 2029790
005 2016-03-17
008 2015 bl --- 0-- u #d
024 7 $a10.1016/j.theriogenology.2015.02.023$2DOI
100 1 $aLEÃO, B. C. S.
245 $aImproved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile.
260 $c2015
520 $aThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species.
650 $aCattle
650 $aconjugated linoleic acid
650 $aIn vitro fertilization
650 $amass spectrometry
650 $aBovino
650 $aEmbrião animal
650 $aReprodução animal
653 $aAnimal embryos
653 $aBovine embryo
653 $aCryosurvival
653 $aIn vitro production
653 $aLipid profile
700 1 $aROCHA-FRIGONI, N. A. S.
700 1 $aCABRAL, E. C.
700 1 $aCOELHO, M. B.
700 1 $aFERREIRA, C. R.
700 1 $aEBERLIN, M. N.
700 1 $aACCORSI, M. F.
700 1 $aNOGUEIRA, E.
700 1 $aMINGOTI, G. Z.
773 $tTheriogenology$gv. 84, n. 1, p. 127-136, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pantanal (CPAP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia. |
|
Data corrente: |
01/09/2016 |
|
Data da última atualização: |
21/02/2017 |
|
Tipo da produção científica: |
Artigo de Divulgação na Mídia |
|
Autoria: |
VAZ JUNIOR, S. |
|
Afiliação: |
SILVIO VAZ JUNIOR, CNPAE. |
|
Título: |
As biorrefinarias e o aproveitamento integral do potencial da biomassa. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Canal Bioenergia, 29 ago. 2016. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Biomassa vegetal; Biorrefinaria. |
|
Thesagro: |
Bioenergia. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147017/1/Artigo-As-biorrefinarias-e-o-aproveitamento-integral-do-potencial-da-biomassa-Canal-Bioenergia.pdf
|
|
Marc: |
LEADER 00408nam a2200133 a 4500
001 2052222
005 2017-02-21
008 2016 bl uuuu u00u1 u #d
100 1 $aVAZ JUNIOR, S.
245 $aAs biorrefinarias e o aproveitamento integral do potencial da biomassa.$h[electronic resource]
260 $aCanal Bioenergia, 29 ago. 2016.$c2016
650 $aBioenergia
653 $aBiomassa vegetal
653 $aBiorrefinaria
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
