Data/hora: 23/06/2024 10:59:55

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 29/09/2015
Data da última atualização: | 20/09/2017
Autoria: | SANTOS, D. F. K. dos; ISTVAN, P.; NORONHA, E. F.; QUIRINO, B. F.; KRÜGER, R. H.
Afiliação: | Débora Farage Knupp dos Santos, UnB; Paula Istvan, UnB; Eliane Ferreira Noronha, UnB; BETANIA FERRAZ QUIRINO, CNPAE; Ricardo Henrique Krüger, UnB.
Título: | New dioxygenase from metagenomic library from Brazilian soil: insights into antibiotic resistance and bioremediation.
Ano de publicação: | 2015
Fonte/Imprenta: | Biotechnology Letters, v. 37, n. 9, p.1809-1817, 2015.
DOI: | 10.1007/s10529-015-1861-x
Idioma: | Inglês
Conteúdo: | Objectives Putative new dioxygenases were identified in a metagenomic b-lactam-resistance screening and, given their key role on aromatic metabolism, we raise the hypothesis that these enzymes maybe concomitantly related to antibiotic resistance and aromatic degradation. Results ORFs of three putative dioxygenases were isolated from resistant metagenomic clones. One of them, CRB2(1), was subcloned into pET24a expression vector and subjected to downstream phenotypic and bioinformatics analyses that demonstrated the ??dual effect?? of our metagenomic dioxygenase, on antibiotic and aromatic resistance. Furthermore, initial characterization assays strongly suggests that CRB2(1) open-reading frame is an extradiol-dioxygenase, most probably a bicupin domain gentisate 1,2-dioxygenase. This observation is, to our knowledge, the first description of a metagenomic dioxygenase and its action on b-lactam resistance. Conclusion Unraveling the diversity of antibiotic resistance elements on the environment could not only identify new genes and mechanisms in which bacteria can resist to antibiotics, but also contribute to biotechnology processes, such as in bioremediation.
Palavras-Chave: | Aromatic metabolism; Aromatic metabolismo; Dioxygenase; Dioxygenase Metagenome; Metagenome; Resistência aos antibióticos.
Thesagro: | Solo.
Thesaurus Nal: | antibiotic resistance; soil.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)

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