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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Clima Temperado; Embrapa Meio Ambiente; Embrapa Soja; Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
Data corrente: |
18/07/2000 |
Data da última atualização: |
16/09/2019 |
Tipo da produção científica: |
Documentos |
Autoria: |
SEMINÁRIO BRASILEIRO DE PRODUÇÃO INTEGRADA DE FRUTAS, 2., 2000, Bento Gonçalves. |
Título: |
Anais... |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Bento Gonçalves: Embrapa Uva e Vinho, 2000. |
Páginas: |
100 p. |
Série: |
(Embrapa Uva e Vinho. Documentos, 28) |
Idioma: |
Português |
Conteúdo: |
Qualidade ambiental e Producao Integrada de Frutas (PIF) no Sub-Medio do Rio Sao Francisco, Petrolina (PE) e Juazeiro (BA), Brasil; Sistemas de inspeccion y certificacion de produccion integrada de frutas; Sistema de certificacion de produccion integrada de frutas en Argentina; Plan management in integrated fruit production; Mercado diferenciado de frutas de produccion integrada en Europa; Mercado diferenciado de frutas de produccion integrada en Argentina; Novas estrategias de pesquisa e desenvolvimento na producao integrada de frutas (PIF); Projeto de pesquisa em producao integrada de manga; Projeto de pesquisa em producao integrada de uvas finas de mesa, Outras estrategais de pesquisa e desenvolvimento na producao integrada de frutas; Resultados de pesquisa da producao integrada de frutas de caroco (PIFC); Avaliacao do sistema de producao integrada de pessego de mesa na Serra do RS - safra 1999/2000; Avaliacao do sistema de producao integrada de pessego de conserva na regiao de Pelotas - safra 1999/2000; Avaliacao do sistema de producao integrada de pessego de mesa na regiao Metropolitana de Porto Alegre - safra 1999/2000; Resultados de pesquisa da producao integrada de maca (PIM); Doencas e pragas em producao integrada de maca (PIM); Manejo da planta e do solo nos sistemas convencional e integrado de macieira; Manejo pos-colheita em macas produzidas nos sistemas convencional e integrado. |
Palavras-Chave: |
Bento Gonçalves; Brasil; Frutas; Integrated control; Integrated plant production; Pesquisa e Desenvolvimento; Producao vegetal integrada; Production; Rio Grande do Sul. |
Thesagro: |
Controle de Qualidade; Controle Integrado; Fitotecnia; Fruta; Maçã; Mercado; Pêssego; Produção; Produção Integrada; Sistema de Produção. |
Thesaurus Nal: |
Brazil; farming systems; fruits; marketing. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/60739/1/CNPUV-DOC.-28-00.pdf
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Marc: |
LEADER 02521nam a2200409 a 4500 001 1538674 005 2019-09-16 008 2000 bl uuuu u01u1 u #d 100 1 $aSEMINÁRIO BRASILEIRO DE PRODUÇÃO INTEGRADA DE FRUTAS, 2., 2000, Bento Gonçalves. 245 $aAnais... 260 $aBento Gonçalves: Embrapa Uva e Vinho$c2000 300 $a100 p. 490 $a(Embrapa Uva e Vinho. Documentos, 28) 520 $aQualidade ambiental e Producao Integrada de Frutas (PIF) no Sub-Medio do Rio Sao Francisco, Petrolina (PE) e Juazeiro (BA), Brasil; Sistemas de inspeccion y certificacion de produccion integrada de frutas; Sistema de certificacion de produccion integrada de frutas en Argentina; Plan management in integrated fruit production; Mercado diferenciado de frutas de produccion integrada en Europa; Mercado diferenciado de frutas de produccion integrada en Argentina; Novas estrategias de pesquisa e desenvolvimento na producao integrada de frutas (PIF); Projeto de pesquisa em producao integrada de manga; Projeto de pesquisa em producao integrada de uvas finas de mesa, Outras estrategais de pesquisa e desenvolvimento na producao integrada de frutas; Resultados de pesquisa da producao integrada de frutas de caroco (PIFC); Avaliacao do sistema de producao integrada de pessego de mesa na Serra do RS - safra 1999/2000; Avaliacao do sistema de producao integrada de pessego de conserva na regiao de Pelotas - safra 1999/2000; Avaliacao do sistema de producao integrada de pessego de mesa na regiao Metropolitana de Porto Alegre - safra 1999/2000; Resultados de pesquisa da producao integrada de maca (PIM); Doencas e pragas em producao integrada de maca (PIM); Manejo da planta e do solo nos sistemas convencional e integrado de macieira; Manejo pos-colheita em macas produzidas nos sistemas convencional e integrado. 650 $aBrazil 650 $afarming systems 650 $afruits 650 $amarketing 650 $aControle de Qualidade 650 $aControle Integrado 650 $aFitotecnia 650 $aFruta 650 $aMaçã 650 $aMercado 650 $aPêssego 650 $aProdução 650 $aProdução Integrada 650 $aSistema de Produção 653 $aBento Gonçalves 653 $aBrasil 653 $aFrutas 653 $aIntegrated control 653 $aIntegrated plant production 653 $aPesquisa e Desenvolvimento 653 $aProducao vegetal integrada 653 $aProduction 653 $aRio Grande do Sul
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Hortaliças. |
Data corrente: |
09/08/2022 |
Data da última atualização: |
11/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
MARTINS, T. P.; REGO, C. M.; NAKASU, E. Y. T.; FERNANDES, F. R.; INOUE-NAGATA, A. K. |
Afiliação: |
T. P. MARTINS, UNIVERSIDADE DE BRASÍLIA; C. M. REGO, UNIVERSIDADE DE BRASÍLIA; ERICH YUKIO TEMPEL NAKASU, CNPH; FERNANDA RAUSCH FERNANDES, CNPTIA; ALICE KAZUKO INOUE NAGATA, CNPH. |
Título: |
A high viral diversity in tomato crops in Brazil is revealed by next generation sequencing analyses. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Acta Horticulturae, v. 1316, p. 99-105, 2021. |
ISSN: |
2406-6168 |
DOI: |
10.17660/ActaHortic.2021.1316.14 |
Idioma: |
Inglês |
Notas: |
Edition of Proceedings of the VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change. |
Conteúdo: |
Tomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed and unreported viruses in tomatoes, such as an amalgavirus and an ilarvirus, were also detected and are under confirmation. The conclusion was that there is a high virus diversity present in tomato plants in Brazil, making tomato production a challenge to the growers. The crinivirus, Tomato chlorosis virus, was the most frequently found within the samplings, suggesting that it is widespread in the major tomato production areas. Two begomoviruses were detected, implying that this strategy is also useful to detect viruses with a DNA genome. Finally, this technique was particularly convenient to identify the viruses coexisting in tomatoes and to find unknown viruses that may threaten the tomato production in the country. MenosTomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed a... Mostrar Tudo |
Palavras-Chave: |
HTS; Next Generation Sequencing; Sequenciamento de nova geração; Virome. |
Thesagro: |
Tomate; Vírus. |
Thesaurus NAL: |
Solanum lycopersicum. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03214naa a2200289 a 4500 001 2145363 005 2022-08-11 008 2021 bl uuuu u00u1 u #d 022 $a2406-6168 024 7 $a10.17660/ActaHortic.2021.1316.14$2DOI 100 1 $aMARTINS, T. P. 245 $aA high viral diversity in tomato crops in Brazil is revealed by next generation sequencing analyses.$h[electronic resource] 260 $c2021 500 $aEdition of Proceedings of the VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change. 520 $aTomato is planted in Brazil mainly for fresh consumption and tomato paste production. Among the various pathogens that infect tomato plants in Brazil, viruses are particularly important due to their high incidence and the resulting losses caused. Diagnosis of viral diseases usually relies on detection methods directed to known viruses and close variants, either by serology or nucleic acid hybridization/ amplification. However, the advent of next generation sequencing (NGS) facilitated a deep analysis of viral populations, which can be used for identification, assembly and discovery of new viruses. Aiming to estimate the viral diversity present in tomato crops from three states of Brazil, five composite leaf samples were analyzed using NGS. The samples referred as Braz (collected in the Federal District, 2015); Ahol, Toca1, and Toca2 (São Paulo State, 2014), and RNY2 (Minas Gerais State, 2013) were submitted to semi-purification of viral particles and RNA extraction before RNA-seq (Illumina). The reads were filtered for quality, the contigs assembled (Velvet algorithm), and submitted to MegaBLAST analysis against a virus reference sequences database. These samples were collected from plants showing symptoms such as mosaic, chlorosis, leaf curling, chlorotic spots, necrosis and stunting. Known viruses belonging to nine genera, Crinivirus, Begomovirus, Tospovirus, Tobravirus, Potyvirus, Tobamovirus, Tymovirus, Potexvirus and Cucumovirus, were detected. Potentially undescribed and unreported viruses in tomatoes, such as an amalgavirus and an ilarvirus, were also detected and are under confirmation. The conclusion was that there is a high virus diversity present in tomato plants in Brazil, making tomato production a challenge to the growers. The crinivirus, Tomato chlorosis virus, was the most frequently found within the samplings, suggesting that it is widespread in the major tomato production areas. Two begomoviruses were detected, implying that this strategy is also useful to detect viruses with a DNA genome. Finally, this technique was particularly convenient to identify the viruses coexisting in tomatoes and to find unknown viruses that may threaten the tomato production in the country. 650 $aSolanum lycopersicum 650 $aTomate 650 $aVírus 653 $aHTS 653 $aNext Generation Sequencing 653 $aSequenciamento de nova geração 653 $aVirome 700 1 $aREGO, C. M. 700 1 $aNAKASU, E. Y. T. 700 1 $aFERNANDES, F. R. 700 1 $aINOUE-NAGATA, A. K. 773 $tActa Horticulturae$gv. 1316, p. 99-105, 2021.
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