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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. |
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Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biomedical Materials, v. 14, article 035011, 2019. |
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DOI: |
https://doi.org/10.1088/1748-605x/ab0a52 |
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Idioma: |
Inglês |
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Conteúdo: |
Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation. MenosNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic... Mostrar Tudo |
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Palavras-Chave: |
3D scaffolds; Human stem cells; Nanomaterial; Nanotopography; Osteoinduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02473naa a2200313 a 4500
001 2118648
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1088/1748-605x/ab0a52$2DOI
100 1 $aAMARAL, D. L.
245 $aIn vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.$h[electronic resource]
260 $c2019
520 $aNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.
653 $a3D scaffolds
653 $aHuman stem cells
653 $aNanomaterial
653 $aNanotopography
653 $aOsteoinduction
700 1 $aZANETTE, R. S.
700 1 $aALMEIDA, C. G.
700 1 $aALMEIDA, L. B.
700 1 $aOLIVEIRA, L. F.
700 1 $aMARCOMINI, R. F.
700 1 $aNOGUEIRA, B. V.
700 1 $aSANTOS, M. O.
700 1 $aBRANDAO, H. de M.
700 1 $aMARANDUBA, C. M. C.
700 1 $aMUNK, M.
773 $tBiomedical Materials$gv. 14, article 035011, 2019.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
03/05/2013 |
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Data da última atualização: |
08/11/2022 |
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Autoria: |
ZARIN, D. J.; DAVIDSON, E. A.; BRONDIZIO, E.; VIEIRA, I. C. G.; SÁ, T.; FELDPAUSCH, T.; SCHUUR, E. A. G.; MESQUITA, R.; MORAN, E.; DELAMONICA, P.; DUCEY, M. J.; HURTT, G. C.; SALIMON, C.; DENICH, M. |
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Afiliação: |
DANIEL J. ZARIN, UNVERSITY OF FLORIDA; ERIC A. DAVIDSON, WOODS HOLE RESEARCH CENTER; EDUARDO BRONDIZIO, INDIANA UNIVERSITY; IMA C. G. VIEIRA, MPEG; TATIANA DEANE DE ABREU SA, CPATU; TED FELDPAUSCH, CORNELL UNIVERSITY; EDWARD A. G. SCHUUR, UNIVERSITY OF FLORIDA; RITA MESQUITA, INPA; EMILIO MORAN, INDIANA UNIVERSITY; PATRICIA DELAMONICA, UNIVERSITY OF FLORIDA; MARK J. DUCEY, UNIVERISTY OF NEW HAMPSHIRE; GEORGE C. HURTT, UNIVERSITY OF NEW HAMPSHIRE; CLEBER SALIMON, USP; MANFRED DENICH, UNIVERISTY OF BONN. |
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Título: |
Legacy of fire slows carbon accumulation in Amazonian forest regrowth. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Frontiers in Ecology and the Environment, v. 3, n. 7, p. 365-369, Sep. 2005. |
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DOI: |
http://dx.doi.org/10.1890/1540-9295(2005)003%5B0365:LOFSCA%5D2.0.CO;2 |
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Idioma: |
Inglês |
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Conteúdo: |
Amazonian farmers and ranchers use fire to clear land for agriculture and pasture as part of extensive land-use strategies that have deforested 500 000 km2 over the past 25 years. Ash from burning biomass fertilizes crops and pastures, but declining productivity often occurs after a few years, generally leading to land abandonment and further clearing. Subsequent forest regrowth partially offsets carbon emissions from deforestation, but is often repeatedly cleared and burned. In the first quantitative, basin-wide assessment of the effect of repeated clearing and burning on forest regrowth, our analysis of data from 93 stands at nine locations across the region indicates that stands with a history of five or more fires suffer on average a greater than 50% reduction in carbon accumulation. In the absence of management interventions, Amazonian landscapes dominated by this pronounced legacy of fire are apt to accumulate very little carbon and will remain highly susceptible to recurrent burning. |
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Thesagro: |
Carbono; Floresta; Queimada. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01969naa a2200337 a 4500
001 1957021
005 2022-11-08
008 2005 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1890/1540-9295(2005)003%5B0365:LOFSCA%5D2.0.CO;2$2DOI
100 1 $aZARIN, D. J.
245 $aLegacy of fire slows carbon accumulation in Amazonian forest regrowth.$h[electronic resource]
260 $c2005
520 $aAmazonian farmers and ranchers use fire to clear land for agriculture and pasture as part of extensive land-use strategies that have deforested 500 000 km2 over the past 25 years. Ash from burning biomass fertilizes crops and pastures, but declining productivity often occurs after a few years, generally leading to land abandonment and further clearing. Subsequent forest regrowth partially offsets carbon emissions from deforestation, but is often repeatedly cleared and burned. In the first quantitative, basin-wide assessment of the effect of repeated clearing and burning on forest regrowth, our analysis of data from 93 stands at nine locations across the region indicates that stands with a history of five or more fires suffer on average a greater than 50% reduction in carbon accumulation. In the absence of management interventions, Amazonian landscapes dominated by this pronounced legacy of fire are apt to accumulate very little carbon and will remain highly susceptible to recurrent burning.
650 $aAmazonia
650 $aCarbono
650 $aFloresta
650 $aQueimada
700 1 $aDAVIDSON, E. A.
700 1 $aBRONDIZIO, E.
700 1 $aVIEIRA, I. C. G.
700 1 $aSÁ, T.
700 1 $aFELDPAUSCH, T.
700 1 $aSCHUUR, E. A. G.
700 1 $aMESQUITA, R.
700 1 $aMORAN, E.
700 1 $aDELAMONICA, P.
700 1 $aDUCEY, M. J.
700 1 $aHURTT, G. C.
700 1 $aSALIMON, C.
700 1 $aDENICH, M.
773 $tFrontiers in Ecology and the Environment$gv. 3, n. 7, p. 365-369, Sep. 2005.
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