Data/hora: 22/09/2024 20:36:30

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Suínos e Aves.	
Data corrente: 	09/09/2021	
Data da última atualização: 	15/10/2021	
Autoria: 	HUL, L. M.; IBELLI, A. M. G.; SAVOLDI, I. R.; MARCELINO, D. E. P.; FERNANDES, L. T.; PEIXOTO, J. O.; CANTAO, M. E.; HIGA, R. H.; GIACHETTO, P. F.; COUTINHO, L. L.; LEDUR, M. C.	
Afiliação: 	LUDMILA MUDRI HUL, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, UDESC; DÉBORA ESTER PETRY MARCELINO, FACULDADE DE CONCÓRDIA; LANA TEIXEIRA FERNANDES; JANE OLIVEIRA PEIXOTO, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; MAURICIO EGIDIO CANTAO, CNPSA; ROBERTO HIROSHI HIGA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; MONICA CORREA LEDUR, CNPSA.	
Título: 	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Scientific Reports, v. 11, n. 1, p. 1-13. 2021.	
DOI: 	https://doi.org/10.1038/s41598-021-97306-3	
Idioma: 	Inglês	
Notas: 	Article number: 17965.	
Conteúdo: 	Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.	
Palavras-Chave: 	Análises de expressão diferencial; Cartilagem da cabeça do fêmur; Differential expression analysis; Expressão gênica; Reação da cadeia de polimerase; Separação da cabeça femoral; Sequenciamento de RNA; Transcriptoma da cartilagem do fêmur.	
Thesagro: 	Frango; Frango de Corte.	
Thesaurus Nal: 	Broiler chickens; Gene expression; Quantitative polymerase chain reaction; Transcriptome.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/226988/1/final9712.pdf	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPTIA - (20945-1) - UPC - AP - DD -  -  -  - DIS
CNPSA - (22224-1) - UPC - AP - DD -  -  -  - DIS