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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/01/2006 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARNEIRO, N. P.; HUGHES, P. A.; LARKINS, B. A. |
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Afiliação: |
NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
The eEF1A gene family is differentially expressed in maize endosperm. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Plant Molecular Biology, Dordrecht, v. 41, n. 6, p. 801-813, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
The elongation factor protein eEF1A appears to be a multifunctional protein in eukaryotes, where it serves as a protein synthesis factor as well as a cytoskeletal protein. In maize endosperm, the eEF1A concentration is highly correlated with lysine content, and eEF1A synthesis is increased in opaque2 mutants compared to wild type plants. To investigate the basis for the increased synthesis of eEF1A in opaque2, genes encoding this protein were characterized and their relative level of expression in endosperm and other tissues was investigated. Maize contains 10 to 15 eEF1A genes that are nearly identical in nucleotide and amino acid sequences. However, these genes can be distinguished based on their 3' non-coding sequences, which are less conserved. By screening endosperm and seedling cDNA libraries, it was shown that the relative level of transcripts of maize eEF1A genes varies in different tissues. At least five genes are transcribed in the endosperm, with 2 accounting for ca. 80% of the RNA transcripts. The expression of several genes is enhanced in opaque2 endosperm, although the significance of this is unclear. Nucleotide sequence data have been submitted to the EMBL/DDBJ/GenBank databases under the accession numbers AF136823 to AF136829 for eEF1Aa, eEF1Ab, eEF1Ac, eEF1Ad, eEF1Ae, eEF1Af and eEF1Ag, respectively.. |
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Palavras-Chave: |
Maize. |
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Thesagro: |
Milho; Zea mays. |
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Thesaurus NAL: |
endosperm. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01879naa a2200193 a 4500
001 1489191
005 2018-05-28
008 1999 bl uuuu u00u1 u #d
100 1 $aCARNEIRO, N. P.
245 $aThe eEF1A gene family is differentially expressed in maize endosperm.$h[electronic resource]
260 $c1999
520 $aThe elongation factor protein eEF1A appears to be a multifunctional protein in eukaryotes, where it serves as a protein synthesis factor as well as a cytoskeletal protein. In maize endosperm, the eEF1A concentration is highly correlated with lysine content, and eEF1A synthesis is increased in opaque2 mutants compared to wild type plants. To investigate the basis for the increased synthesis of eEF1A in opaque2, genes encoding this protein were characterized and their relative level of expression in endosperm and other tissues was investigated. Maize contains 10 to 15 eEF1A genes that are nearly identical in nucleotide and amino acid sequences. However, these genes can be distinguished based on their 3' non-coding sequences, which are less conserved. By screening endosperm and seedling cDNA libraries, it was shown that the relative level of transcripts of maize eEF1A genes varies in different tissues. At least five genes are transcribed in the endosperm, with 2 accounting for ca. 80% of the RNA transcripts. The expression of several genes is enhanced in opaque2 endosperm, although the significance of this is unclear. Nucleotide sequence data have been submitted to the EMBL/DDBJ/GenBank databases under the accession numbers AF136823 to AF136829 for eEF1Aa, eEF1Ab, eEF1Ac, eEF1Ad, eEF1Ae, eEF1Af and eEF1Ag, respectively..
650 $aendosperm
650 $aMilho
650 $aZea mays
653 $aMaize
700 1 $aHUGHES, P. A.
700 1 $aLARKINS, B. A.
773 $tPlant Molecular Biology, Dordrecht$gv. 41, n. 6, p. 801-813, 1999.
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