|
|
Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
08/01/2024 |
Data da última atualização: |
08/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAÇÃO, S. M. B.; LEITE, T. F.; BUDZINSKI, I. G. F.; SANTOS, T. B.; SCHOLZ, M. B. S.; CARPENTIERI-PIPOLO, V.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. |
Afiliação: |
S. M. B. CAÇÃO, INSTITUTO AGRONÔMICO DO PARANÁ; T. F. LEITE, INSTITUTO AGRONÔMICO DO PARANÁ; I. G. F. BUDZINSKI, INSTITUTO AGRONÔMICO DO PARANÁ; T. B. DOS SANTOS, INSTITUTO AGRONÔMICO DO PARANÁ; M. B. S. SCHOLZ, INSTITUTO AGRONÔMICO DO PARANÁ; V. CARPENTIERI-PIPOLO, UNIVERSIDADE ESTADUAL DE LONDRINA; D. S. DOMINGUES, INSTITUTO AGRONÔMICO DO PARANÁ; L. G. E. VIEIRA, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa. |
Título: |
Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3186-3197, 2012. |
DOI: |
http://dx.doi.org/10.4238/2012.September.3.7 |
Idioma: |
Inglês |
Conteúdo: |
Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring during coffee fruit maturation, the enzymatic activity of PME was measured during different stages of fruit ripening. PME activity progressively increased from the beginning of the ripening process to the cherry fruit stage. In silico analysis of expressed sequence tags of the Brazilian Coffee Genome Project database identified 5 isoforms of PME. We isolated and cloned a cDNA homolog of PME for further characterization. CaPME4 transcription was analyzed in pericarp, perisperm, and endosperm tissues during fruit development and ripening as well as in other plant tissues. Northern blot analysis revealed increased transcription of CaPME4 in the pericarp 300 days after flowering. Low levels of CaPME4 mRNAs were observed in the endosperm 270 days after flowering. Expression of CaPME4 transcripts was strong in the branches and lower in root and flower tissues. We showed that CaPME4 acts specifically during the later stages of fruit ripening and possibly contributes to the softening of coffee fruit, thus playing a significant role in pectin degradation in the fruit pericarp. |
Thesagro: |
Coffea Arábica. |
Thesaurus Nal: |
Gene expression; Plant development; Ripening; Seed maturation; Seed quality. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160536/1/Gene-expression.pdf
|
Marc: |
LEADER 02430naa a2200301 a 4500 001 2160536 005 2024-01-08 008 2012 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.4238/2012.September.3.7$2DOI 100 1 $aCAÇÃO, S. M. B. 245 $aGene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.$h[electronic resource] 260 $c2012 520 $aCoffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring during coffee fruit maturation, the enzymatic activity of PME was measured during different stages of fruit ripening. PME activity progressively increased from the beginning of the ripening process to the cherry fruit stage. In silico analysis of expressed sequence tags of the Brazilian Coffee Genome Project database identified 5 isoforms of PME. We isolated and cloned a cDNA homolog of PME for further characterization. CaPME4 transcription was analyzed in pericarp, perisperm, and endosperm tissues during fruit development and ripening as well as in other plant tissues. Northern blot analysis revealed increased transcription of CaPME4 in the pericarp 300 days after flowering. Low levels of CaPME4 mRNAs were observed in the endosperm 270 days after flowering. Expression of CaPME4 transcripts was strong in the branches and lower in root and flower tissues. We showed that CaPME4 acts specifically during the later stages of fruit ripening and possibly contributes to the softening of coffee fruit, thus playing a significant role in pectin degradation in the fruit pericarp. 650 $aGene expression 650 $aPlant development 650 $aRipening 650 $aSeed maturation 650 $aSeed quality 650 $aCoffea Arábica 700 1 $aLEITE, T. F. 700 1 $aBUDZINSKI, I. G. F. 700 1 $aSANTOS, T. B. 700 1 $aSCHOLZ, M. B. S. 700 1 $aCARPENTIERI-PIPOLO, V. 700 1 $aDOMINGUES, D. S. 700 1 $aVIEIRA, L. G. E. 700 1 $aPEREIRA, L. F. P. 773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3186-3197, 2012.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Café (CNPCa) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
26/11/2010 |
Data da última atualização: |
26/11/2010 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FINGER, P. F.; DUMMER, L. A.; CUNHA, C. E. P.; CONCEIÇÃO, F. R.; MUNHOZ, L. S.; NUNES, C, F.; VIANCELLI, A.; ESTEVES, P. A.; FISCHER, G.; VARGAS, G. D.; HÜBNER, S. O. |
Afiliação: |
UFPEL; UFPEL; UFPEL; UFPEL; UFPEL; UFPEL; PAULO AUGUSTO ESTEVES, CNPSA; UFPEL; UFPEL; UFPEL. |
Título: |
Construction of expression vector pPICZalfaB for cloning of S1 glycoprotein gene of the infectious bronchitis virus (IBV) in Pichia pastoris. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Virus Reviews and Research, v. 15, supl. 1, p. 305, oct. 2010. Trabalho apresentado no NATIONAL MEETING OF VIROLOGY, 21.; MERCOSUL MEETING OF VIROLOGY, 5. |
Idioma: |
Inglês |
Notas: |
Projeto/Plano de Ação: 03.09.01800-01. |
Palavras-Chave: |
Virologia animal. |
Thesagro: |
Gene. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00919naa a2200265 a 4500 001 1868111 005 2010-11-26 008 2010 bl uuuu u00u1 u #d 100 1 $aFINGER, P. F. 245 $aConstruction of expression vector pPICZalfaB for cloning of S1 glycoprotein gene of the infectious bronchitis virus (IBV) in Pichia pastoris. 260 $c2010 500 $aProjeto/Plano de Ação: 03.09.01800-01. 650 $aGene 653 $aVirologia animal 700 1 $aDUMMER, L. A. 700 1 $aCUNHA, C. E. P. 700 1 $aCONCEIÇÃO, F. R. 700 1 $aMUNHOZ, L. S. 700 1 $aNUNES, C, F. 700 1 $aVIANCELLI, A. 700 1 $aESTEVES, P. A. 700 1 $aFISCHER, G. 700 1 $aVARGAS, G. D. 700 1 $aHÜBNER, S. O. 773 $tVirus Reviews and Research$gv. 15, supl. 1, p. 305, oct. 2010. Trabalho apresentado no NATIONAL MEETING OF VIROLOGY, 21.; MERCOSUL MEETING OF VIROLOGY, 5.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|