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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
06/07/2021 |
Data da última atualização: |
28/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MACHADO, R. C.; SILVA, A. B. S. da; ANDRADE, D. F. de; PINHEIRO, F. C.; WILLIAMS, C. B.; DONATI, G. L.; NOGUEIRA, A. R. de A.; PEREIRA FILHO, E. R. |
Afiliação: |
RAQUEL CARDOSO MACHADO, UFSCAR; ANA BEATRIZ SANTOS DA SILVA, UFSCAR; DANIEL FERNANDES DE ANDRADE, UFSCAR; FERNANDA COSTA PINHEIRO, UFSCAR; CHARLES B. WILLIAMS, Davidson College; GEORGE LUIS DONATI, Wake Forest University; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; EDENIR RODRIGUES PEREIRA FILHO, UFSCAR. |
Título: |
Alternative approaches applied to inductively coupled plasma techniques: multi-flow and two-flow calibration. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of the Brazilian Chemical Society, v.32, n.4, 2021. |
Páginas: |
p.842-851. |
ISSN: |
0103-5053 |
DOI: |
https://dx.doi.org/10.21577/0103-5053.20200235 |
Idioma: |
Inglês |
Conteúdo: |
Multi-flow calibration (MFC) is based on monitoring the analytical signal from a single calibration standard solution at several different nebulization gas flow rates (Q), which normalizes plasma conditions and minimizes matrix effects. In the present study, MFC was evaluated, for the first time, applied to inductively coupled plasma optical emission spectrometry (ICP OES) and inductively coupled plasma mass spectrometry (ICP-MS) to analyze different and complexmatrix samples. Al, As, Cd, Cr, Cu, Mn, Pb, and Zn were determined by ICP OES, while As, Cd, Co, Cu, Hg, Mo, Mn, Ni, Se, Sb, Pb, and V were determined by ICP-MS. MFC results were compared with those obtained using external standard calibration (EC) for both techniques and in all cases, MFC showed equal or superior accuracy (recoveries between 80-120%) compared to EC, and lower relative standard deviation (RSDs < 10%). Several tests were also performed using only two nebulization gas flows to build linear models for calibration (called two-flow calibration, TFC) and the accurate results (recoveries ranged from 80 to 110% for ICP OES and from 81 to 102% for ICP-MS) suggests that this strategy can be also applied, resulting in a method with high sample throughput. |
Palavras-Chave: |
Calibration strategy; ICP OES; ICP-MS; Matrix effects; Nebulization gas. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224314/1/Alternative-Approaches-Applied.pdf
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Marc: |
LEADER 02162naa a2200301 a 4500 001 2132812 005 2021-09-28 008 2021 bl uuuu u00u1 u #d 022 $a0103-5053 024 7 $ahttps://dx.doi.org/10.21577/0103-5053.20200235$2DOI 100 1 $aMACHADO, R. C. 245 $aAlternative approaches applied to inductively coupled plasma techniques$bmulti-flow and two-flow calibration.$h[electronic resource] 260 $c2021 300 $ap.842-851. 520 $aMulti-flow calibration (MFC) is based on monitoring the analytical signal from a single calibration standard solution at several different nebulization gas flow rates (Q), which normalizes plasma conditions and minimizes matrix effects. In the present study, MFC was evaluated, for the first time, applied to inductively coupled plasma optical emission spectrometry (ICP OES) and inductively coupled plasma mass spectrometry (ICP-MS) to analyze different and complexmatrix samples. Al, As, Cd, Cr, Cu, Mn, Pb, and Zn were determined by ICP OES, while As, Cd, Co, Cu, Hg, Mo, Mn, Ni, Se, Sb, Pb, and V were determined by ICP-MS. MFC results were compared with those obtained using external standard calibration (EC) for both techniques and in all cases, MFC showed equal or superior accuracy (recoveries between 80-120%) compared to EC, and lower relative standard deviation (RSDs < 10%). Several tests were also performed using only two nebulization gas flows to build linear models for calibration (called two-flow calibration, TFC) and the accurate results (recoveries ranged from 80 to 110% for ICP OES and from 81 to 102% for ICP-MS) suggests that this strategy can be also applied, resulting in a method with high sample throughput. 653 $aCalibration strategy 653 $aICP OES 653 $aICP-MS 653 $aMatrix effects 653 $aNebulization gas 700 1 $aSILVA, A. B. S. da 700 1 $aANDRADE, D. F. de 700 1 $aPINHEIRO, F. C. 700 1 $aWILLIAMS, C. B. 700 1 $aDONATI, G. L. 700 1 $aNOGUEIRA, A. R. de A. 700 1 $aPEREIRA FILHO, E. R. 773 $tJournal of the Brazilian Chemical Society$gv.32, n.4, 2021.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
09/07/2013 |
Data da última atualização: |
19/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MELZER, M. J.; SIMBAJON, N.; CARILLO, J.; BORTH, W. B.; ASTUA, J. de F.; KITAJIMA, E. W.; NEUPANE, K. R.; HU, J. S. |
Afiliação: |
MICHAEL J. MELZER, University of Hawaii; NELSON SIMBAJON, University of Hawaii; JAMES CARILLO, University of Hawaii; WAYNE B. BORTH, University of Hawaii; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT W. KITAJIMA, ESALQ; KABI R. NEUPANE, University of Hawaii; JOHN S. HU, University of Hawaii. |
Título: |
A cilevirus infects ornamental hibiscus in Hawaii. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Archives of Virology, June. 2013. |
ISSN: |
0304-8608 |
DOI: |
10.1007/s00705-013-1745-0 |
Idioma: |
Inglês |
Conteúdo: |
The complete nucleotide sequence of a virus infecting ornamental hibiscus (Hibiscus sp.) in Hawaii with symptoms of green ringspots on senescing leaves was determined from double-stranded RNA isolated from symptomatic tissue. Excluding polyadenylated regions at the 3? termini, the bipartite RNA genome was 8748 and 5019 nt in length for RNA1 and RNA2, respectively. The genome organization was typical of a cilevirus: RNA1 encoded a large replication-associated protein with methyltransferase, protease, helicase and RNA-dependent RNA polymerase domains as well as a 29-kDa protein of unknown function. RNA2 possessed five open reading frames that potentially encoded proteins with molecular masses of 15, 7, 62, 32, and 24 kDa. The 32-kDa protein is homologous to 3A movement proteins of RNA viruses; the other proteins are of unknown function. A proteome comparison revealed that this virus was 92 % identical to citrus leprosis virus cytoplasmic type 2 (CiLV-C2), a recently characterized cilevirus infecting citrus with leprosis-like symptoms in Colombia. The high sequence similarity suggests that the virus described in this study could be a strain of CiLV-C2, but since the new genus Cilevirus does not have species demarcation criteria established at present, the classification of this virus infecting hibiscus is open to interpretation. This study represents the first documented case of a cilevirus established in the United States and provides insight into the diversity within the genus Cilevirus. MenosThe complete nucleotide sequence of a virus infecting ornamental hibiscus (Hibiscus sp.) in Hawaii with symptoms of green ringspots on senescing leaves was determined from double-stranded RNA isolated from symptomatic tissue. Excluding polyadenylated regions at the 3? termini, the bipartite RNA genome was 8748 and 5019 nt in length for RNA1 and RNA2, respectively. The genome organization was typical of a cilevirus: RNA1 encoded a large replication-associated protein with methyltransferase, protease, helicase and RNA-dependent RNA polymerase domains as well as a 29-kDa protein of unknown function. RNA2 possessed five open reading frames that potentially encoded proteins with molecular masses of 15, 7, 62, 32, and 24 kDa. The 32-kDa protein is homologous to 3A movement proteins of RNA viruses; the other proteins are of unknown function. A proteome comparison revealed that this virus was 92 % identical to citrus leprosis virus cytoplasmic type 2 (CiLV-C2), a recently characterized cilevirus infecting citrus with leprosis-like symptoms in Colombia. The high sequence similarity suggests that the virus described in this study could be a strain of CiLV-C2, but since the new genus Cilevirus does not have species demarcation criteria established at present, the classification of this virus infecting hibiscus is open to interpretation. This study represents the first documented case of a cilevirus established in the United States and provides insight into the diversity within the genu... Mostrar Tudo |
Palavras-Chave: |
Cilevirus gênero. |
Thesaurus NAL: |
Cilevirus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02181naa a2200253 a 4500 001 1961664 005 2023-05-19 008 2013 bl uuuu u00u1 u #d 022 $a0304-8608 024 7 $a10.1007/s00705-013-1745-0$2DOI 100 1 $aMELZER, M. J. 245 $aA cilevirus infects ornamental hibiscus in Hawaii.$h[electronic resource] 260 $c2013 520 $aThe complete nucleotide sequence of a virus infecting ornamental hibiscus (Hibiscus sp.) in Hawaii with symptoms of green ringspots on senescing leaves was determined from double-stranded RNA isolated from symptomatic tissue. Excluding polyadenylated regions at the 3? termini, the bipartite RNA genome was 8748 and 5019 nt in length for RNA1 and RNA2, respectively. The genome organization was typical of a cilevirus: RNA1 encoded a large replication-associated protein with methyltransferase, protease, helicase and RNA-dependent RNA polymerase domains as well as a 29-kDa protein of unknown function. RNA2 possessed five open reading frames that potentially encoded proteins with molecular masses of 15, 7, 62, 32, and 24 kDa. The 32-kDa protein is homologous to 3A movement proteins of RNA viruses; the other proteins are of unknown function. A proteome comparison revealed that this virus was 92 % identical to citrus leprosis virus cytoplasmic type 2 (CiLV-C2), a recently characterized cilevirus infecting citrus with leprosis-like symptoms in Colombia. The high sequence similarity suggests that the virus described in this study could be a strain of CiLV-C2, but since the new genus Cilevirus does not have species demarcation criteria established at present, the classification of this virus infecting hibiscus is open to interpretation. This study represents the first documented case of a cilevirus established in the United States and provides insight into the diversity within the genus Cilevirus. 650 $aCilevirus 653 $aCilevirus gênero 700 1 $aSIMBAJON, N. 700 1 $aCARILLO, J. 700 1 $aBORTH, W. B. 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 700 1 $aNEUPANE, K. R. 700 1 $aHU, J. S. 773 $tArchives of Virology, June. 2013.
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