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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
03/09/2021 |
Data da última atualização: |
03/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PACHECO, T. F.; MACHADO, B. R. C.; MORAIS JÚNIOR, W. G. de; ALMEIDA, J. R. M. de; GONCALVES, S. B. |
Afiliação: |
THALYTA FRAGA PACHECO, CNPAE; BRENO R. C. MACHADO; WILSON G. DE MORAIS JÚNIOR, Instituto Politécnico do Porto; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; SILVIA BELEM GONCALVES, CNPAE. |
Título: |
Enhanced tolerance of Spathaspora passalidarum to sugarcane bagasse hydrolysate for ethanol production from xylose. |
Complemento do título: |
. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Applied Biochemistry and Biotechnology, v. 193, n. 7, p. 2182-2197, July 2021. |
Descrição Física: |
PDF: il. |
DOI: |
https://doi.org/10.1007/s12010-021-03544-6 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released. These compounds inhibit yeast metabolism, reducing fermentation yields and productivity. This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate. Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors. Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate. At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate. A kinetic model was developed to describe the inhibition of fermentation by substrate and product. The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L. Ethanol concentration that stops cell growth was 30.1 g/L. There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%. |
Palavras-Chave: |
Spathaspora passalidarum. |
Thesagro: |
Biomassa; Cana de Açúcar; Hidrolise. |
Thesaurus Nal: |
Biomass; Evolutionary adaptation; Hydrolysates; Lignocellulose; Sugarcane; Xylose. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02441naa a2200313 a 4500 001 2134053 005 2021-09-03 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s12010-021-03544-6$2DOI 100 1 $aPACHECO, T. F. 245 $aEnhanced tolerance of Spathaspora passalidarum to sugarcane bagasse hydrolysate for ethanol production from xylose.$h[electronic resource] 260 $c2021 300 $cPDF: il. 520 $aAbstract: During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released. These compounds inhibit yeast metabolism, reducing fermentation yields and productivity. This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate. Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors. Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate. At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate. A kinetic model was developed to describe the inhibition of fermentation by substrate and product. The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L. Ethanol concentration that stops cell growth was 30.1 g/L. There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%. 650 $aBiomass 650 $aEvolutionary adaptation 650 $aHydrolysates 650 $aLignocellulose 650 $aSugarcane 650 $aXylose 650 $aBiomassa 650 $aCana de Açúcar 650 $aHidrolise 653 $aSpathaspora passalidarum 700 1 $aMACHADO, B. R. C. 700 1 $aMORAIS JÚNIOR, W. G. de 700 1 $aALMEIDA, J. R. M. de 700 1 $aGONCALVES, S. B. 773 $tApplied Biochemistry and Biotechnology$gv. 193, n. 7, p. 2182-2197, July 2021.
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Embrapa Agroenergia (CNPAE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
14/01/2010 |
Data da última atualização: |
14/01/2010 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
COSTA, A. A. G.; LUDKE, M. do C. M. M.; LUDKE, J. V.; HOLANDA, A. C. de; HOLANDA, M. C. R. de; OLIVEIRA, E. L. de; TORRES, T. R. |
Afiliação: |
UFRPE; UFRPE; JORGE VITOR LUDKE, CNPSA; UFRPE; UFRPE; UFRPE; UFRPE. |
Título: |
Viabilidade econômica do uso de farinha de penas hidrolisadas na ração de frango de corte fêmea |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 9; SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA, 6; 2009, Recife. Anais.. Recife: UFRPE, 2009. 1 CD-ROM. |
Idioma: |
Português |
Notas: |
Projeto/Plano de Ação: 16.00.30004-00 |
Palavras-Chave: |
Farinha de pena hidrolisada; Reaproveitamento. |
Thesagro: |
Custo; Economia; Nutrição. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00872naa a2200253 a 4500 001 1580478 005 2010-01-14 008 2009 bl --- 0-- u #d 100 1 $aCOSTA, A. A. G. 245 $aViabilidade econômica do uso de farinha de penas hidrolisadas na ração de frango de corte fêmea 260 $c2009 500 $aProjeto/Plano de Ação: 16.00.30004-00 650 $aCusto 650 $aEconomia 650 $aNutrição 653 $aFarinha de pena hidrolisada 653 $aReaproveitamento 700 1 $aLUDKE, M. do C. M. M. 700 1 $aLUDKE, J. V. 700 1 $aHOLANDA, A. C. de 700 1 $aHOLANDA, M. C. R. de 700 1 $aOLIVEIRA, E. L. de 700 1 $aTORRES, T. R. 773 $tIn: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 9; SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA, 6; 2009, Recife. Anais.. Recife: UFRPE, 2009. 1 CD-ROM.
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