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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/10/2008 |
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Data da última atualização: |
02/10/2008 |
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Autoria: |
SANTOS, E. M. R.; FRANKLIN, E.; MAGNUSSON, W. E. |
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Título: |
Cost-efficiency of Sub-sampling Protocols to Evaluate Oribatid-Mite Communities in an Amazonian Savanna. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Sampling oribatid mites in large areas using conventional methods is expensive, time-consuming,
and this constrains their use in environmental monitoring programs. We used samples collected
in 38 plots of 3.75 ha spread over 30,000 ha in an Amazonian savanna to evaluate the reduction
in costs and person-hours in sampling and sorting and to elaborate cost-effective protocols. Ten
samples per plot were collected and extracted using a Berlese-Tullgren apparatus. In the
laboratory, samples were reduced to 50, 25, 12.5 and 6.25 percent of the initial content. Fieldeffort
reduction was estimated by reducing the number of subsamples per plot. Dissimilarity
matrices were generated using Bray-Curtis, Sørensen and Chao-Sørensen indices. Correlations
between each reduced-effort dissimilarity matrix and 100 or 50 percent sorting were used as an
index of how much information was retained in reduced-effort sampling, and could still be used
in multivariate analyses. The effects of most predictor variables on mite composition were
detected in data based on every level of sample reduction. The intensive sampling was insufficient
to reveal the full oribatid-mite fauna in the savanna; as more plots were sampled, more species
were recorded. Our data indicate sub-sampling protocols for biodiversity assessment of oribatid
mites in savanna that increase field and labor efficiency, and optimize both taxonomic and
ecological aspects of the investigation.
Financial support: PPG7 6400-0021-00, PPI-INPA 1-3010, CNPq. MenosSampling oribatid mites in large areas using conventional methods is expensive, time-consuming,
and this constrains their use in environmental monitoring programs. We used samples collected
in 38 plots of 3.75 ha spread over 30,000 ha in an Amazonian savanna to evaluate the reduction
in costs and person-hours in sampling and sorting and to elaborate cost-effective protocols. Ten
samples per plot were collected and extracted using a Berlese-Tullgren apparatus. In the
laboratory, samples were reduced to 50, 25, 12.5 and 6.25 percent of the initial content. Fieldeffort
reduction was estimated by reducing the number of subsamples per plot. Dissimilarity
matrices were generated using Bray-Curtis, Sørensen and Chao-Sørensen indices. Correlations
between each reduced-effort dissimilarity matrix and 100 or 50 percent sorting were used as an
index of how much information was retained in reduced-effort sampling, and could still be used
in multivariate analyses. The effects of most predictor variables on mite composition were
detected in data based on every level of sample reduction. The intensive sampling was insufficient
to reveal the full oribatid-mite fauna in the savanna; as more plots were sampled, more species
were recorded. Our data indicate sub-sampling protocols for biodiversity assessment of oribatid
mites in savanna that increase field and labor efficiency, and optimize both taxonomic and
ecological aspects of the investigation.
Financial support: PPG7 6400-0021-00, PPI-INP... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02225naa a2200145 a 4500
001 1315002
005 2008-10-02
008 2008 bl uuuu u00u1 u #d
100 1 $aSANTOS, E. M. R.
245 $aCost-efficiency of Sub-sampling Protocols to Evaluate Oribatid-Mite Communities in an Amazonian Savanna.
260 $c2008
520 $aSampling oribatid mites in large areas using conventional methods is expensive, time-consuming, and this constrains their use in environmental monitoring programs. We used samples collected in 38 plots of 3.75 ha spread over 30,000 ha in an Amazonian savanna to evaluate the reduction in costs and person-hours in sampling and sorting and to elaborate cost-effective protocols. Ten samples per plot were collected and extracted using a Berlese-Tullgren apparatus. In the laboratory, samples were reduced to 50, 25, 12.5 and 6.25 percent of the initial content. Fieldeffort reduction was estimated by reducing the number of subsamples per plot. Dissimilarity matrices were generated using Bray-Curtis, Sørensen and Chao-Sørensen indices. Correlations between each reduced-effort dissimilarity matrix and 100 or 50 percent sorting were used as an index of how much information was retained in reduced-effort sampling, and could still be used in multivariate analyses. The effects of most predictor variables on mite composition were detected in data based on every level of sample reduction. The intensive sampling was insufficient to reveal the full oribatid-mite fauna in the savanna; as more plots were sampled, more species were recorded. Our data indicate sub-sampling protocols for biodiversity assessment of oribatid mites in savanna that increase field and labor efficiency, and optimize both taxonomic and ecological aspects of the investigation. Financial support: PPG7 6400-0021-00, PPI-INPA 1-3010, CNPq.
700 1 $aFRANKLIN, E.
700 1 $aMAGNUSSON, W. E.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão; Embrapa Territorial. |
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Data corrente: |
20/07/2022 |
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Data da última atualização: |
04/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
HOFFMANN, L. V.; BRANQUINHO, A. A.; BARROSO, P. A. V.; VASLIN, M. F. S. |
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Afiliação: |
LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, article 814119, 5 p. 2022. |
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ISBN: |
1664-462X |
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DOI: |
10.3389/fpls.2022.814119 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. |
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Palavras-Chave: |
CLRDV; Enzyme-linked immunosorbent assay (ELISA); Intermediary host. |
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Thesagro: |
Vírus. |
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Thesaurus NAL: |
Serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144832/1/6046.pdf
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Marc: |
LEADER 01770naa a2200241 a 4500
001 2151489
005 2023-05-04
008 2022 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2022.814119$2DOI
100 1 $aHOFFMANN, L. V.
245 $aAntibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.$h[electronic resource]
260 $c2022
520 $aThe cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.
650 $aSerology
650 $aVírus
653 $aCLRDV
653 $aEnzyme-linked immunosorbent assay (ELISA)
653 $aIntermediary host
700 1 $aBRANQUINHO, A. A.
700 1 $aBARROSO, P. A. V.
700 1 $aVASLIN, M. F. S.
773 $tFrontiers in Plant Science$gv. 13, article 814119, 5 p. 2022.
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Embrapa Algodão (CNPA) |
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