Data/hora: 13/06/2024 11:46:32

Biblioteca(s): 	Embrapa Algodão; Embrapa Territorial.	
Data corrente: 	20/07/2022	
Data da última atualização: 	04/05/2023	
Autoria: 	HOFFMANN, L. V.; BRANQUINHO, A. A.; BARROSO, P. A. V.; VASLIN, M. F. S.	
Afiliação: 	LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO.	
Título: 	Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Frontiers in Plant Science, v. 13, article 814119, 5 p. 2022.	
ISBN: 	1664-462X	
DOI: 	10.3389/fpls.2022.814119	
Idioma: 	Inglês	
Conteúdo: 	The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.	
Palavras-Chave: 	CLRDV; Enzyme-linked immunosorbent assay (ELISA); Intermediary host.	
Thesagro: 	Vírus.	
Thesaurus Nal: 	Serology.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144832/1/6046.pdf	
Registro original: 	Embrapa Algodão (CNPA)	
Exemplares: 	CNPM - (6046-1) - UPC - AP - DD - 22/026 - AP - 2022.026 - DIS
CNPA - (28998-1) - UPC - AP - DD -  -  -  - DIS