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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. |
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Afiliação: |
YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP. |
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Título: |
How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021. |
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ISSN: |
1806-9967 |
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Idioma: |
Português |
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Conteúdo: |
New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance. |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227820/1/How-to-drive-phloem-gene-expression.pdf
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Marc: |
LEADER 02150naa a2200205 a 4500
001 2136203
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
022 $a1806-9967
100 1 $aBEZERRA, Y. C. dos A.
245 $aHow to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.$h[electronic resource]
260 $c2021
520 $aNew approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
650 $aFruta Cítrica
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aATTÍLIO, L. B.
700 1 $aASTUA, J. de F.
700 1 $aMOURÃO FILHO, F. de A. A.
773 $tRevista Brasileira de Fruticultura, Jaboticabal$gv. 43, n. 4,e-005, 2021.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Arroz e Feijão. |
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Data corrente: |
10/12/2020 |
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Data da última atualização: |
16/12/2020 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
MADARI, B. E.; CUADRA, S. V.; OLIVEIRA, P. P. A.; HIGA, R. C. V.; RAMOS, N. P.; ANDRADE, C. A. de; KEMENES, A.; GONDIM, R. S. |
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Afiliação: |
BEATA EMOKE MADARI, CNPAF; SANTIAGO VIANNA CUADRA, CNPTIA; PATRICIA PERONDI ANCHAO OLIVEIRA, CPPSE; ROSANA CLARA VICTORIA HIGA, CNPF; NILZA PATRICIA RAMOS, CNPMA; CRISTIANO ALBERTO DE ANDRADE, CNPMA; ALEXANDRE KEMENES, CPAMN; RUBENS SONSOL GONDIM, CNPAT. |
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Título: |
The role of agriculture in mitigating greenhouse gas emissions. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In: CUADRA, S. V.; HEINEMANN, A. B.; BARIONI, L. G.; MOZZER, G. B.; BERGIER, I. (Ed.). Climate action: contributions of Embrapa. Brasília, DF: Embrapa, 2020. |
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Páginas: |
p. 57-65. |
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Série: |
(Sustainable development goals, 13). |
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ISBN: |
978-65-86056-40-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction. Agriculture and livestock. Energy crops. Forestry sector. Ecological systems. Fish farming. Final considerations. |
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Palavras-Chave: |
Mitigação de emissão de gases do efeito estufa. |
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Thesagro: |
Agricultura; Carbono; Efeito Estufa; Mudança Climática. |
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Thesaurus NAL: |
Agriculture; Carbon sequestration; Climate change; Global warming; Greenhouse gas emissions; Soil organic carbon. |
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Categoria do assunto: |
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P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219286/1/SDG-13-.pdf
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Marc: |
LEADER 01313naa a2200373 a 4500
001 2128223
005 2020-12-16
008 2020 bl uuuu u00u1 u #d
020 $a978-65-86056-40-2
100 1 $aMADARI, B. E.
245 $aThe role of agriculture in mitigating greenhouse gas emissions.$h[electronic resource]
260 $c2020
300 $ap. 57-65.
490 $a(Sustainable development goals, 13).
520 $aIntroduction. Agriculture and livestock. Energy crops. Forestry sector. Ecological systems. Fish farming. Final considerations.
650 $aAgriculture
650 $aCarbon sequestration
650 $aClimate change
650 $aGlobal warming
650 $aGreenhouse gas emissions
650 $aSoil organic carbon
650 $aAgricultura
650 $aCarbono
650 $aEfeito Estufa
650 $aMudança Climática
653 $aMitigação de emissão de gases do efeito estufa
700 1 $aCUADRA, S. V.
700 1 $aOLIVEIRA, P. P. A.
700 1 $aHIGA, R. C. V.
700 1 $aRAMOS, N. P.
700 1 $aANDRADE, C. A. de
700 1 $aKEMENES, A.
700 1 $aGONDIM, R. S.
773 $tIn: CUADRA, S. V.; HEINEMANN, A. B.; BARIONI, L. G.; MOZZER, G. B.; BERGIER, I. (Ed.). Climate action: contributions of Embrapa. Brasília, DF: Embrapa, 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Tipo/Formato |
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