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| 20. |  | UHL, C.; JORDAN, C.; CLARK, K.; CLARK, H.; HERRERA, R. Ecosystem recovery in Amazon caatinga forest after cutting, cutting and burning and bulldozer clearing treatments. Oikos, v.38, n.3, p.313-320, 1982.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 38 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
04/01/2024 |
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Data da última atualização: |
04/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CARVALHO, M. A. de F.; PAIVA, R.; STEIN, V. C.; HERRERA, R. C.; PORTO, J. M. P.; VARGAS, D. P.; ALVES, E. |
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Afiliação: |
MILENE ALVES DE FIGUEIREDO CARVALHO, CNPCa; RENATO PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS; VANESSA CRISTINA STEIN, UNIVERSIDADE FEDERAL DE GOIÁS; RAÍRYS CRAVO HERRERA, UNIVERSIDADE FEDERAL DO PARÁ; JORGE MARCELO PADOVANI PORTO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; DAIANE PEIXOTO VARGAS, EMBRAPA CLIMA TEMPERADO; EDUARDO ALVES, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Induction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014. |
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DOI: |
https://doi.org/10.1590/S1516-8913201402555 |
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Idioma: |
Inglês |
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Conteúdo: |
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli. |
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Thesagro: |
Passifloraceae. |
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Thesaurus NAL: |
Passion fruits; Plant morphology; Tissue culture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160451/1/Induction-and-Morpho.pdf
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Marc: |
LEADER 01945naa a2200253 a 4500
001 2160451
005 2024-01-04
008 2014 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1516-8913201402555$2DOI
100 1 $aCARVALHO, M. A. de F.
245 $aInduction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit$h[electronic resource]
260 $c2014
520 $aThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
650 $aPassion fruits
650 $aPlant morphology
650 $aTissue culture
650 $aPassifloraceae
700 1 $aPAIVA, R.
700 1 $aSTEIN, V. C.
700 1 $aHERRERA, R. C.
700 1 $aPORTO, J. M. P.
700 1 $aVARGAS, D. P.
700 1 $aALVES, E.
773 $tBrazilian Archives of Biology and Technology$gv. 57, n. 6, p. 851-859, 2014.
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Embrapa Café (CNPCa) |
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