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Registros recuperados : 38 | |
1. | | VARGAS, L. K.; BALDANI, J. I.; HEMERLY, A. S. Association of rice cultivars with azospirillum brasilense and Burkholderia brasilensis: studies on the expression of ethylene receptors. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.24 Biblioteca(s): Embrapa Agrobiologia. |
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8. | | CAMARA, G.; CASANOVA, M. A.; HEMERLY, A. S.; MAGALHÃES, G. C.; MEDEIROS, C. M. B. Anatomia de sistemas de informação geográfica. Campinas: UNICAMP, Instituto de Computação, 1996. 193 p. Trabalho apresentado na Escola de Computação, 10., 1996, Campinas. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Solos. |
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17. | | CARVALHO, T. G.; ROSMAN, A. C.; GRAVITOL, C.; NOGUEIRA, E. de M.; BALDANI, J. I.; HEMERLY, A. S. genotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways. Plants, v. 11, n. 15, 1971, 2022. Biblioteca(s): Embrapa Agrobiologia. |
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19. | | ROUWS, L. F. M.; SCHWAB, S.; GUEDES, H. V.; TEIXEIRA, K. R. dos S.; HEMERLY, A. S.; BALDANI, J. I. Isolation of Gluconacetobacter diazotrophicus flagellar motility mutants. In: ANNUAL MEETING OF SBBq, 37., CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia. Abstracts... Águas de Lindóia: SBBq, 2008. 1 p. Parceria: UFRRJ; UFRJ; Laboratório de Biologia Molecular de Plantas-Jardim Botânico, RJ. Biblioteca(s): Embrapa Agrobiologia. |
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20. | | NOGUEIRA, E. de M.; OLIVARES, F. L.; JAPIASSU, J. C.; VILAR, C.; VINAGRE, F.; BALDANI, J. I.; HEMERLY, A. S. Characterization of glutamine synthetase genes in sugarcane genotypes with different rates of biological nitrogen fixation. Plant Science, Ireland, v. 169, n. 5, p. 819-832, nov. 2005. Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 38 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
22/04/2008 |
Data da última atualização: |
16/03/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
ROUWS, L. F. M.; SIMÕES ARAÚJO, J. L.; HEMERLY, A. S.; BALDANI, J. I. |
Afiliação: |
Luc F. M. Rouws, UFRRJ; Jean Luis Simões Araújo, Embrapa Agrobiologia; Adriana S. Hemerly, Instituto de Pesquisas do Jardim Botânico do RJ; José Ivo Baladani, Embrapa Agrobiologia. |
Título: |
Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Archives of Microbiology, New York, v. 189, n. 4, p. 397-405, apr. 2008. |
Idioma: |
Inglês |
Notas: |
Parceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. |
Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. |
Palavras-Chave: |
Bactéria endofítica. |
Thesaurus NAL: |
Gluconacetobacter diazotrophicus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01936naa a2200193 a 4500 001 1626792 005 2015-03-16 008 2008 bl uuuu u00u1 u #d 100 1 $aROUWS, L. F. M. 245 $aValidation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. 260 $c2008 500 $aParceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. 520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?<KAN-2>Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. 650 $aGluconacetobacter diazotrophicus 653 $aBactéria endofítica 700 1 $aSIMÕES ARAÚJO, J. L. 700 1 $aHEMERLY, A. S. 700 1 $aBALDANI, J. I. 773 $tArchives of Microbiology, New York$gv. 189, n. 4, p. 397-405, apr. 2008.
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