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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/06/1994 |
Data da última atualização: |
15/02/2017 |
Autoria: |
BARBOSA, J. S. |
Título: |
Administracao rural a nivel de fazendeiro. |
Edição: |
2.ed. |
Ano de publicação: |
0 |
Fonte/Imprenta: |
Sao Paulo: Nobel, s.d. |
Páginas: |
98p. |
Idioma: |
Português |
Conteúdo: |
Conceitos de organizacao rural; Metas e riscos da exploracao rural; Necessidades de planejamento e controle na organizacao rural; O planejamento na exploracao rural; Os controles da organizacao rural; Modelos. |
Palavras-Chave: |
Fazendeiro. |
Thesagro: |
Administração Agrícola; Administração Rural; Fazenda. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00654nam a2200181 a 4500 001 1472026 005 2017-02-15 008 bl uuuu 00u1 u #d 100 1 $aBARBOSA, J. S. 245 $aAdministracao rural a nivel de fazendeiro. 250 $a2.ed. 260 $aSao Paulo: Nobel, s.d.$c0 300 $a98p. 520 $aConceitos de organizacao rural; Metas e riscos da exploracao rural; Necessidades de planejamento e controle na organizacao rural; O planejamento na exploracao rural; Os controles da organizacao rural; Modelos. 650 $aAdministração Agrícola 650 $aAdministração Rural 650 $aFazenda 653 $aFazendeiro
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Embrapa Milho e Sorgo (CNPMS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
16/12/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
Idioma: |
Inglês |
Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
Palavras-Chave: |
ERIC-PCR; RAPD. |
Thesagro: |
Corynebacterium Pseudotuberculosis. |
Thesaurus NAL: |
caseous lymphadenitis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02507naa a2200265 a 4500 001 1910089 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI 100 1 $aGUIMARAES, A. S. 245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource] 260 $c2011 520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. 650 $acaseous lymphadenitis 650 $aCorynebacterium Pseudotuberculosis 653 $aERIC-PCR 653 $aRAPD 700 1 $aDORNELES, E. M. S. 700 1 $aANDRADE, G. I. 700 1 $aLAGE, A. P. 700 1 $aMIYOSHI, A. 700 1 $aAZEVEDO, V. 700 1 $aGOUVEIA, A. M. G. 700 1 $aHEINEMANN, M. B. 773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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