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Registros recuperados : 38 | |
21. | | PÉREZ, V. K. C.; MAGOSSI, E. M.; OLIVEIRA, P. F. R. e; CUSTODIO, D. A. C.; CRUZ, H. F. DA; ABREU, J. P. DE; HEINEMANN, M. B.; GUIMARÃES, A. S.; DORNELES, E. M. Detection of biofilm-forming Staphylococcus aureus isolated from bovine mastitis and association with antimicrobial resistance in Brazil. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 24., 2018, Santiago, Chile. Resúmenes... Santiago: ALAM, 2018. p. 201. Biblioteca(s): Embrapa Gado de Leite. |
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22. | | SALES, M. L.; FONSECA JUNIOR, A. A.; SALES, E. B.; COTTORELLO, A. C. P.; ISSA, M. A.; HODON, M. A.; SOARES FILHO, P. M.; RAMALHO, A. K.; SILVA, M. R.; LAGE, A. P.; HEINEMANN, M. B. Evaluation of molecular markers for the diagnosis of Mycobacterium bovis Folia Microbiologica, v. 59, n. 5, p. 433-438, 2014. Biblioteca(s): Embrapa Gado de Leite. |
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23. | | DORNELES, E. M. S.; FONSECA, M. D. A. M.; ABREU, J. A. P.; LAGE, A. P.; BRITO, M. A. V. P.; PEREIRA, C. R.; BRANDAO, H. de M.; GUIMARÃES, A. S.; HEINEMANN, M. B. Genetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil. Microbiology Open, v. 8, n. 5, article e736, 2019. Biblioteca(s): Embrapa Gado de Leite. |
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24. | | SOUZA-FILHO, A. F. de; OSÓRIO, A. L. A. R.; JORGE, K. dos S. G.; ARAUJO, F. R.; VIDAL, C. E. S.; ARAÚJO, C. P.; ALBERTTI, L. A. G.; CAZOLA, D. de O.; FERREIRA NETO, J. S.; HEINEMANN, M. B. Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil. Semina: Ciências Agrárias, Londrina, v. 37, n. 5, suplemento 2, p. 3719-3726, 2016 Título em português: Perfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do Brasil. Biblioteca(s): Embrapa Gado de Corte. |
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25. | | GOMES, A. A. de B.; BERNARDI, F.; OLIVEIRA, A. G. de F.; ITO, F. H.; MAIORKA, P. C.; SOARES, R. M. S.; HEINEMANN, M. B.; CORTEZ, A.; RICHTZENHAIN, L. J. Raiva experimental em caprinos e ovinos inoculados com uma amostra de vírus isolada de raposa (Dusicyon vetulus). Ciência Veterinária nos Trópicos, Recife, v. 8, n. 1/3, p. 24-34, jan./dez., 2005. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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26. | | DORNELES, E. M. S.; MORCATTI, F. C.; GUIMARÃES, A. de S.; LOBATO, Z. I. P.; LAGE, A. P.; GONÇALVES, V. S. P.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalence of bluetongue virus antibodies in sheep from Distrito Federal, Brazil. Semina: Ciências Agrárias, Londrina, v. 33, n. 4, p. 1521-1524, jul./ago., 2012. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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27. | | CARMO, F. B. DO; GUIMARAES, A. S.; PAULETTI, R. B.; LAGE, A. P.; GONÇALVES, V. S. P.; MEYER, R.; PORTELA, R. W. B.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra a linfadenite caseosa em criações comerciais de ovinos no Distrito Federal, Brasil. Arquivos do Instituto Biológico, v. 79, n. 2, p. 293-298, 2012. Biblioteca(s): Embrapa Gado de Leite. |
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28. | | CARMO, F. B. do; GUIMARÃES, A. S.; PAULETTI, R. B.; LAGE, A. P.; GONÇALVES, V. S. P.; MEYER, R.; PORTELA, R. W. D.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra a linfadenite caseosa em criações comerciais de ovinos no Distrito Federal, Brasil. Arquivos do Instituto Biológico, v. 79, n. 2, p. 293-298, abr./jun. 2012. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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29. | | CARMO, F. B. do; GUIMARÃES, A. de S.; PAULETTI, R. B.; LAGE, A. P.; GOUVEIA, A. M. G.; FERREIRA, F.; PINHEIRO, R. R.; PORTELA, R. W. D.; AZEVEDO, V. A. de C.; HEINEMANN, M. B. Soroprevalência da linfadenite caseosa em caprinos em propriedades no estado do Ceará In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25.,2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Resumo 2103-1. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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30. | | SALES, M. L.; FONSECA JUNIOR, A. A.; ORZIL, L.; ALENCAR, A. P.; HODON, M. A.; ISSA, M. A.; SOARES FILHO, P. M.; SILVA, M. R.; LAGE, A. P.; HEINEMANN, M. B. Validation of two real-time PCRs targeting the PE-PGRS 20 gene and the region of difference 4 for the characterization of Mycobacterium bovis isolates. Genetics and Molecular Research, v. 13, n. 2, p. 4607-16, 2014. Biblioteca(s): Embrapa Gado de Leite. |
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31. | | PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. Biblioteca(s): Embrapa Gado de Leite. |
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32. | | LEAL FILHO, J. M.; BOTTENE, I. F. N.; MONTEIRO, L. A. R. C.; PELLEGRIN, A. O.; GONÇALVES, V. S. P.; FERREIRA, F.; DIAS, R. A.; AMAKU, M.; TELLES, E. O.; GRISI FILHO, J. H. H.; HEINEMANN, M. B.; FERREIRA NETO, J. S. Control of bovine brucellosis from 1998 to 2009 in the state of Mato Grosso do Sul, Brazil. Semina: Ciências Agrárias, Londrina, v. 37, n. 5, suplemento 2, p. 3467-3478, 2016. Biblioteca(s): Embrapa Pantanal. |
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33. | | CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. Biblioteca(s): Embrapa Gado de Leite. |
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34. | | DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. Plos One, v. 9, n. 6, e98758, 2014. Biblioteca(s): Embrapa Gado de Leite. |
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35. | | HOMEM, V. S. F.; HEINEMANN, M. B.; MORAES, Z. M.; VIANNA, M. C. B.; SILVA, J. C. R.; SAKAMOTO, S. M.; PINHEIRO, S. R.; VEIGA, J. B. da; LAU, H. D.; QUANZ, D.; TOURRAND, J.-F.; FERREIRA, F.; FERREIRA NETO, J. S. Leptospirose bovina em Uruará, PA, município da Amazônia Oriental. Arquivos do Instituto Biológico, v. 67, n. 1, p. 1-10, 2000. Biblioteca(s): Embrapa Amazônia Oriental. |
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36. | | DORNELES, E. M. S.; GUIMARÃES, A. S.; GOUVEIA, A. M. G.; COURA, F. M.; CARMO, F. B. do; PAULETTI, R. B.; AZEVEDO, V.; LILENBAUM, W.; VITOR, V. de A.; PINHEIRO, R. R.; FERREIRA, A. C. R.; DASSO, M. G.; LAGE, A. P.; HEINEMANN, M. B. Seroprevalence of Brucella ovis-epididymitis, smooth-Brucella, leptospirosis, toxoplasmosis, and Maedi-Visna in sheep slaughtered in Minas Gerais State, Brazil. Brazilian Journal of Veterinary Research and Animal Science, v. 57, n. 2, e164278, 2020. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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37. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016. Biblioteca(s): Embrapa Unidades Centrais. |
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38. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N. de; LAGE, A. P.; BLAGITZ, M. G.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 38 | |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
18/08/2021 |
Data da última atualização: |
19/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. |
Afiliação: |
VERÓNICA K. C. PÉREZ, Universidade Federal de Lavras, Lavras; DIRCÉIA A. C. CUSTÓDIO, Universidade Federal de Lavras; EDUARDA M. M. SILVA, Universidade Federal de Lavras; JULIA DE OLIVEIRA, Universidade Federal de Lavras; ALESSANDRO DE SA GUIMARAES, CNPGL; MARIA A. V. P. BRITO; ANTÔNIO F. SOUZA-FILHO, Universidade de São Paulo; MARCOS B. HEINEMANN, Universidade de São Paulo; ANDREY P. LAGE, Universidade Federal de Minas Gerais; ELAINE M. S. DORNELES, Universidade Federal de Lavras. |
Título: |
Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. |
DOI: |
https://doi.org/10.1007/s42770-020-00363-5 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. MenosThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), er... Mostrar Tudo |
Palavras-Chave: |
Hemolisina; Infecção intramamária; Intramammary infection; Mastite; Staphylococci. |
Thesagro: |
Biofilme; Bovino; Doença Animal; Estafilococo. |
Thesaurus NAL: |
Biofilm; Hemolysins. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03482naa a2200373 a 4500 001 2133706 005 2021-08-19 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s42770-020-00363-5$2DOI 100 1 $aPEREZ, V. K. C. 245 $aVirulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.$h[electronic resource] 260 $c2020 520 $aThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. 650 $aBiofilm 650 $aHemolysins 650 $aBiofilme 650 $aBovino 650 $aDoença Animal 650 $aEstafilococo 653 $aHemolisina 653 $aInfecção intramamária 653 $aIntramammary infection 653 $aMastite 653 $aStaphylococci 700 1 $aCUSTODIO, D. A. C. 700 1 $aSILVA, E. M. M. 700 1 $aOLIVEIRA, J. de 700 1 $aGUIMARÃES, A. S. 700 1 $aBRITO, M. A. V. P. 700 1 $aSOUZA-FILHO, A. F. 700 1 $aHEINEMANN, M. B. 700 1 $aLAGE, A. P. 700 1 $aDORNELES, E. M. S. 773 $tBrazilian Journal of Microbiology$gv. 51, p. 2111-2122, 2020.
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