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| 16. |  | FISCHER, D.; SCHMID, M.; REIS, V. M.; HARTMANN, A. In situ characterization of endophytic diazotrophs in sugar cane (Saccharum officinarum) based on 16S rRNA diversity. In: RHIZOSPHERE 2, SATELLITE INTERNATIONAL WORKSHOP, 2007, Montpeller. Azospirillum VII and related PGPR: Genomics, molecular ecology, plant responses and agronomic significance. Montpellier: Université de Montpeller: CNRS, 2007. p. 44. Session 2: taxonomy, ecology and biodiversity. Parceria: GSF National Research Center for Environment and Health| Biblioteca(s): Embrapa Agrobiologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
19/01/2006 |
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Data da última atualização: |
08/12/2006 |
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Autoria: |
AZEVEDO, M. dos S.; TEIXEIRA, K. R. dos S.; KIRCHHOF, G.; HARTMANN, A.; BALDANI, J. I. |
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Título: |
Influence of soil and host plant crop on the genetic diversity of Azospirillum amazonense isolates. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Pedobiologia, Jena, v. 49, n. 6, p. 565-576, nov. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
The genetic structure of Azospirillum amazonense populations isolated from the rhizosphere soil and washed and surface-sterilised roots of rice, maize and sorghum plants, cropped silmultaneously in two different soils (clay loam and sandy loam) was characterised. Genetic diversity was measured by restriction fragment length polymorphism of the amplified 16S-23S rDNA intergenic spacer region (RISA-RFLP) and cluster analysis. Four genetically distinct clusters of isolates were observed with 78% similarity, suggesting that the A. amazonense population was heterogeneous at the strain level regardless of the soil type or host plant. Analysis of molecular variance (AMOVA) demonstrated that the host plant had a highly significant selective effect on the genetic structure of this species, especially on those isolates intimately associated with them, but also to a lesser extent on isolates from the rhizosphere and washed roots. The soil type also had a highly significant selective effect on A. amazonense genetic diversity, especially for those isolates from the rhizosphere soil. The selective effect of the soil type combined with that of the host plant suggests that environmental factors, such as soil texture and composition of exudates provided by C3 or C4 plants, play major roles in the overall genetic structure of A. amazonense populations associated with these cereals. |
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Palavras-Chave: |
A; amazonense; Biological nitrogen fixation; BNF; FBN; Fixação biológica de nitrogênio; Soil type; Tipo de solo. |
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Thesagro: |
Bactéria. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02160naa a2200277 a 4500
001 1624128
005 2006-12-08
008 2005 bl --- 0-- u #d
100 1 $aAZEVEDO, M. dos S.
245 $aInfluence of soil and host plant crop on the genetic diversity of Azospirillum amazonense isolates.
260 $c2005
520 $aThe genetic structure of Azospirillum amazonense populations isolated from the rhizosphere soil and washed and surface-sterilised roots of rice, maize and sorghum plants, cropped silmultaneously in two different soils (clay loam and sandy loam) was characterised. Genetic diversity was measured by restriction fragment length polymorphism of the amplified 16S-23S rDNA intergenic spacer region (RISA-RFLP) and cluster analysis. Four genetically distinct clusters of isolates were observed with 78% similarity, suggesting that the A. amazonense population was heterogeneous at the strain level regardless of the soil type or host plant. Analysis of molecular variance (AMOVA) demonstrated that the host plant had a highly significant selective effect on the genetic structure of this species, especially on those isolates intimately associated with them, but also to a lesser extent on isolates from the rhizosphere and washed roots. The soil type also had a highly significant selective effect on A. amazonense genetic diversity, especially for those isolates from the rhizosphere soil. The selective effect of the soil type combined with that of the host plant suggests that environmental factors, such as soil texture and composition of exudates provided by C3 or C4 plants, play major roles in the overall genetic structure of A. amazonense populations associated with these cereals.
650 $aBactéria
653 $aA
653 $aamazonense
653 $aBiological nitrogen fixation
653 $aBNF
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aSoil type
653 $aTipo de solo
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aKIRCHHOF, G.
700 1 $aHARTMANN, A.
700 1 $aBALDANI, J. I.
773 $tPedobiologia, Jena$gv. 49, n. 6, p. 565-576, nov. 2005.
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