Data/hora: 21/06/2024 10:33:28

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 29/06/2021
Data da última atualização: | 01/07/2021
Autoria: | AZEVEDO, B. T.; VERCESI FILHO, A. E.; GUTMANIS, G.; VERISSIMO, C. J.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; GIGLIOTI, R.
Afiliação: | BIANCA TAINA AZEVEDO, IZ; ANIBAL EUGENIO VERCESI FILHO, IZ; GUNTA GUTMANIS, IZ; CECÍLIA JOSE VERISSIMO, IZ; LUCIANA MORITA KATIKI, IZ; CINTIA HIROMI OKINO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; RODRIGO GIGLIOTI, IZ.
Título: | New sensitive methods for fraud detection in buffalo dairy products.
Ano de publicação: | 2021
Fonte/Imprenta: | International Dairy Journal, v.117, 105013, jun. 2021.
Páginas: | 9 p.
DOI: | https://doi.org/10.1016/j.idairyj.2021.105013
Idioma: | Inglês
Conteúdo: | The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp® SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required.
Palavras-Chave: | DNA extraction; High resolution melting; Real time PCR; RhAmpR SNP system.
Thesaurus Nal: | Buffalo milk.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (25344-1) - UPC - AP - DD - PROCI-2021.00077 - AZE - 2021.00084 - DIS