Data/hora: 12/06/2024 05:33:17

Biblioteca(s): | Embrapa Gado de Leite.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 01/04/2022
Data da última atualização: | 01/04/2022
Autoria: | PILLAI, V. V.; KEI, T. G.; GURUNG, S.; DAS, M.; SIQUEIRA, L. G. B.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V.
Afiliação: | VIJU VIJAYAN PILLAI, Cornell University; TIFFANY G. KEI, Cornell University; SHAILESH GURUNG, Cornell University; MOUBANI DAS, Cornell University; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; SOON HON CHEONG, Cornell University; PETER J. HANSEN, University of Florida; VIMAL SELVARAJ, Cornell University.
Título: | RhoA/ROCK signaling antagonizes bovine trophoblast stem cell self-renewal and regulates preimplantation embryo size and differentiation.
Ano de publicação: | 2022
Fonte/Imprenta: | Development, v. 149, n. 7, dev200115, 2022.
DOI: | https://doi.org/10.1242/dev.200115
Idioma: | Inglês
Conteúdo: | Exponential proliferation of trophoblast stem cells (TSC) is crucial in Ruminantia to maximize numerical access to caruncles, the restricted uterine sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed transforming growth factor &#946;1 (TGF&#946;1) as a primary upstream effector. TGF&#946;1 treatment induced changes consistent with differentiation in bTSCs, a response that could be replicated by induced expression of the bovine ROCK2 transgene. Rocki could partially antagonize TGF&#946;1 effects, and TGF&#946; receptor inhibition promoted proliferation identical to Rocki, indicating an all-encompassing upstream regulation. Morphological differentiation included formation of binucleate cells and infrequent multinucleate syncytia, features we also localize in the in vivo bovine placenta. Collectively, we demonstrate a central role for TGF&#946;1, RhoA and Rock in inducing bTSC differentiation, attenuation of which is sufficient to sustain self-renewal and proliferation linked to blastocyst size and preimplantation development. Unraveling these mechanisms augments evolutionary/comparative physiology of the trophoblast cell lineage and placental development in eutherians.
Palavras-Chave: | Blastocisto; Células-tronco; Trofoblasto.
Thesagro: | Bovino; Embrião Animal; Reprodução Animal.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (25610-1) - UPC - AP - DD -  -  -  - DIS