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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/10/2018 |
Data da última atualização: |
16/10/2018 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
MONDIN, M.; SEREJO, J. A. dos S.; BERTÃO, M. R.; LABORDA, P.; PIZZAIA, D.; AGUIAR-PERECIN, M. L. R. |
Afiliação: |
MATEUS MONDIN, ESALQ; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; MÔNICA R. BERTÃO, ESALQ; PRIANDA LABORDA, UNICAMP; DANIEL PIZZAIA, ESALQ; MARGARIDA L. R. AGUIAR-PERECIN, ESALQ. |
Título: |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with kys standard line. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: TOP 10 Contributions on Plant Biology. Local: Telangana, India: Avid Science, 2018. |
Idioma: |
Inglês |
Conteúdo: |
Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise q MenosMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes.... Mostrar Tudo |
Palavras-Chave: |
Heterochromatic Knobs. |
Thesagro: |
DNA; Milho. |
Thesaurus Nal: |
Centromeres; Chromosomes; Satellite DNA. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184478/1/Karyotype-Variability-in-Tropical-Maize-Sister-Inbred-Lines-and-Hybrids-Compared-with-KYS-Standard-Line.pdf
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Marc: |
LEADER 02643naa a2200253 a 4500 001 2097537 005 2018-10-16 008 2018 bl uuuu u00u1 u #d 100 1 $aMONDIN, M. 245 $aKaryotype variability in tropical maize sister inbred lines and hybrids compared with kys standard line.$h[electronic resource] 260 $c2018 520 $aMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise q 650 $aCentromeres 650 $aChromosomes 650 $aSatellite DNA 650 $aDNA 650 $aMilho 653 $aHeterochromatic Knobs 700 1 $aSEREJO, J. A. dos S. 700 1 $aBERTÃO, M. R. 700 1 $aLABORDA, P. 700 1 $aPIZZAIA, D. 700 1 $aAGUIAR-PERECIN, M. L. R. 773 $tIn: TOP 10 Contributions on Plant Biology. Local: Telangana, India: Avid Science, 2018.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
06/09/2002 |
Data da última atualização: |
07/06/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
PEREIRA, R. C.; MAKI, C. S.; OLIVEIRA, E.; SOUZA, I. R. P.; GUIMARAES, C. T.; CARNEIRO, N. P.; PAIVA, E. |
Afiliação: |
ELIZABETH DE OLIVEIRA SABATO, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
Título: |
Caracterização de grãos de amido durante o enchimento de grãos em milho. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis, SC. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Florianópolis: Epagri, 2002. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Opaco-2; QPM. |
Thesagro: |
Amido; Endosperma; Milho. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/33863/1/Caracterizacao-graos.pdf
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Marc: |
LEADER 00839nam a2200241 a 4500 001 1485689 005 2018-06-07 008 2002 bl uuuu u00u1 u #d 100 1 $aPEREIRA, R. C. 245 $aCaracterização de grãos de amido durante o enchimento de grãos em milho.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis, SC. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Florianópolis: Epagri$c2002 300 $c1 CD-ROM. 650 $aAmido 650 $aEndosperma 650 $aMilho 653 $aOpaco-2 653 $aQPM 700 1 $aMAKI, C. S. 700 1 $aOLIVEIRA, E. 700 1 $aSOUZA, I. R. P. 700 1 $aGUIMARAES, C. T. 700 1 $aCARNEIRO, N. P. 700 1 $aPAIVA, E.
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Embrapa Milho e Sorgo (CNPMS) |
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