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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
07/12/2015 |
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Data da última atualização: |
26/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, P. M.; GUIMARAES, L. A.; MORGANTE, C. V.; SILVA JUNIOR, O. B.; ARAUJO, A. C. G.; MARTINS, A. C. Q.; SARAIVA, M. A. P.; OLIVEIRA, T. N.; TOGAWA, R. C.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D. J.; BRASILEIRO, A. C. M. |
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Afiliação: |
PATRICIA MESSEMBERG GUIMARAES, CENARGEN; LARISSA A. GUIMARAES; CAROLINA VIANNA MORGANTE, CPATSA; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; ANDRESSA C. Q. MARTINS, UNB; MARIO ALFREDO DE PASSOS SARAIVA, CENARGEN; THAIS N. OLIVEIRA, UNB; ROBERTO COITI TOGAWA, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; DAVID J. BERTIOLI, UNB; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
Root transcriptome analysis of wild peanut reveals candidate genes for nematode resistance. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, October 21, p. 1-22, 2015. (Open Access). |
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DOI: |
10.1371/journal.pone.0140937 |
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Idioma: |
Inglês |
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Conteúdo: |
Wild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132 contigs and 37,882 loci. Differentially expressed genes (DEGs) were identified and clustered according to their expression profile, with the majority being downregulated at 6 DAI, which coincides with the onset of the HR. Amongst these DEGs, 27 were selected for further qRT-PCR validation allowing the identifi- cation of nematode-responsive candidate genes that are putatively related to the resistance response. Those candidates are engaged in the salycilic (NBS-LRR, lipocalins, resveratrol synthase) and jasmonic (patatin, allene oxidase cyclase) acids pathways, and also related to hormonal balance (auxin responsive protein, GH3) and cellular plasticity and signaling (tetraspanin, integrin, expansin), with some of them showing contrasting expression behav-ior between Arachis RKN-resistant and susceptible genotypes. As these candidate genes activate different defensive signaling systems, the genetic (HR) and the induced resistance(IR), their pyramidding in one genotype via molecular breeding or transgenic strategy might contribute to a more durable resistance, thus improving the long-term control of RKN in peanut. MenosWild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132 contigs and 37,882 loci. Differentially expressed genes (DEGs) were identified and clustered according to their expression profile, with the majority being downregulated at 6 DAI, which coincides with the onset of the HR. Amongst these DEGs, 27 were selected for further qRT-PCR validation allowing the identifi- cation of nematode-responsive candidate genes that are putatively related to the resistance response. Those candidates are engaged in the salycilic (NBS-LRR, lipocalins, resveratrol synthase) and jasmonic (patatin, allene oxidase cyclase) acids pathways, and also related to hormonal balance (auxin responsive protein, GH3) and cellular plasticity and signaling (tetraspanin, integrin, expansin), with some of them showing co... Mostrar Tudo |
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Palavras-Chave: |
Arachis spp; Diplóide selvagem; Gene regulador; Melhoramento de planta; Nematóide das galhas; Peanut; Peanut relatives; Planta resistente. |
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Thesagro: |
Arachis Hypogaea. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134987/1/pone.0140937.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140762/1/Morgante.pdf
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Marc: |
LEADER 02999naa a2200373 a 4500
001 2030792
005 2024-04-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0140937$2DOI
100 1 $aGUIMARAES, P. M.
245 $aRoot transcriptome analysis of wild peanut reveals candidate genes for nematode resistance.$h[electronic resource]
260 $c2015
520 $aWild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132 contigs and 37,882 loci. Differentially expressed genes (DEGs) were identified and clustered according to their expression profile, with the majority being downregulated at 6 DAI, which coincides with the onset of the HR. Amongst these DEGs, 27 were selected for further qRT-PCR validation allowing the identifi- cation of nematode-responsive candidate genes that are putatively related to the resistance response. Those candidates are engaged in the salycilic (NBS-LRR, lipocalins, resveratrol synthase) and jasmonic (patatin, allene oxidase cyclase) acids pathways, and also related to hormonal balance (auxin responsive protein, GH3) and cellular plasticity and signaling (tetraspanin, integrin, expansin), with some of them showing contrasting expression behav-ior between Arachis RKN-resistant and susceptible genotypes. As these candidate genes activate different defensive signaling systems, the genetic (HR) and the induced resistance(IR), their pyramidding in one genotype via molecular breeding or transgenic strategy might contribute to a more durable resistance, thus improving the long-term control of RKN in peanut.
650 $aArachis Hypogaea
653 $aArachis spp
653 $aDiplóide selvagem
653 $aGene regulador
653 $aMelhoramento de planta
653 $aNematóide das galhas
653 $aPeanut
653 $aPeanut relatives
653 $aPlanta resistente
700 1 $aGUIMARAES, L. A.
700 1 $aMORGANTE, C. V.
700 1 $aSILVA JUNIOR, O. B.
700 1 $aARAUJO, A. C. G.
700 1 $aMARTINS, A. C. Q.
700 1 $aSARAIVA, M. A. P.
700 1 $aOLIVEIRA, T. N.
700 1 $aTOGAWA, R. C.
700 1 $aLEAL-BERTIOLI, S. C. M.
700 1 $aBERTIOLI, D. J.
700 1 $aBRASILEIRO, A. C. M.
773 $tPlos One, October 21, p. 1-22, 2015. (Open Access).
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 3 | |
| 1. |  | SOUZA, M. do C. P. de; MAIA, G. A.; GUEDES, Z. B. de L.; ORIA, H. F.; HOLANDA, L. F. F. de. Amadurecimento natural e artificial do buriti. Pesquisa Agropecuaria Brasileira, Brasilia, v.19, n.7, p. 891-896, jul. 1984.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | MAIA, G. A.; ARAUJO, C. N. L. de; NUNES, R. de P.; GUEDES, Z. B. de L.; MONTEIRO, J. C. S. Composição química de doze cultivares de girassol obtidas sob condições climáticas do Estado do Ceará. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 29, n. 8, p. 1273-1280. ago. 1994. Título em inglês: Chemical composition of twelve sunflower cultivars grown in Ceará, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | ARAÚJO, C. N. L. de; MAIA, G. A.; NUNES, R. de P.; GUEDES, Z. B. de L.; MONTEIRO, J. C. S. Caracterização do óleo das sementes de doze genótipos de girassol (Helianthus annuus, L.) obtidos sob condições climáticas do estado do Ceará. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 29, n. 6, p. 901-906, jun. 1994. Título em inglês: Caracterization of seed oil from 12 genotypes of sunflower (Helianthus annuus, L.) obtained unde the climatic conditions of the State of Ceará.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 3 | |
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