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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
21/03/2022 |
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Data da última atualização: |
22/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALLENTI, R.; HUSSEIN, H. E.; ALZAN, H. F.; SUAREZ, C. E.; UETI, M.; ASURMENDI, S.; BENITEZ, D.; ARAUJO, F. R.; ROLLS, P.; SIBEKO-MATJILA, K.; SCHNITTGER, L.; FLORIN-CHRISTENSEN, M. |
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Afiliação: |
ROMINA GALLENTI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; HALA E. HUSSEIN, Washington State University, USA.; HEBA F. ALZAN, Washington State University, USA.; CARLOS E. SUAREZ, Washington State University, USA.; MASSARO UETI, Washington State University, USA.; SEBASTIÁN ASURMENDI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; DANIEL BENITEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.; FLABIO RIBEIRO DE ARAUJO, CNPGC; PETER ROLLS, Department of Agriculture & Fisheries, Tick Fever Centre, Australia.; KGOMOTSO SIBEKO-MATJILA, University of Pretoria, South Africa.; LEONHARD SCHNITTGER, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; MÓNICA FLORIN-CHRISTENSEN, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina. |
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Título: |
Unraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pathogens, v. 11, n. 344, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen. |
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Palavras-Chave: |
Inter-strain polymorphism; Rhomboid serine proteases. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus Nal: |
Babesiosis; Gene expression; Tick-borne diseases. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232800/1/2022-Babesia-ROM4.pdf
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Marc: |
LEADER 02337naa a2200325 a 4500
001 2141112
005 2022-03-22
008 2022 bl uuuu u00u1 u #d
100 1 $aGALLENTI, R.
245 $aUnraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies.$h[electronic resource]
260 $c2022
520 $aBabesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen.
650 $aBabesiosis
650 $aGene expression
650 $aTick-borne diseases
650 $aBabesia Bovis
653 $aInter-strain polymorphism
653 $aRhomboid serine proteases
700 1 $aHUSSEIN, H. E.
700 1 $aALZAN, H. F.
700 1 $aSUAREZ, C. E.
700 1 $aUETI, M.
700 1 $aASURMENDI, S.
700 1 $aBENITEZ, D.
700 1 $aARAUJO, F. R.
700 1 $aROLLS, P.
700 1 $aSIBEKO-MATJILA, K.
700 1 $aSCHNITTGER, L.
700 1 $aFLORIN-CHRISTENSEN, M.
773 $tPathogens$gv. 11, n. 344, 2022.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
31/10/2002 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FILHO, M. M.; DELLA LUCIA, T. M. C.; CRUZ, I.; GUEDES, R. N. C.; GALVAO, J. C. C. |
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Afiliação: |
IVAN CRUZ, CNPMS. |
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Título: |
Chlorpyrifos sprying of no-tillage corn during tasselling and its effect on damage by Helicoverpa zea (Lep., Noctuidae) and on its natural enemies. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Applied Entomology, Hamburg, v. 126, n. 7/8, p. 422-430, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Corn plants were twice sprayed at 3-day intervals during tasselling to evaluate the effect of chlorpyrifos on ear damage by H . zea and also on the populations of the natural enemies associated with this moth. The abundance of H . zea eggs and larvae and of predators on the whole plant, the egg parasitism by Trichogramma pretiosum Riley and the percentage of injured ears were sampled weekly. Chlorpyrifos did not show any significant effect on population levels and on ear damage by H . zea . Natural parasitism of eggs ranged from 52 to 75% and did not differ between treated and untreated plots; furthermore, the percentage of non viable eggs and adult emergence were not influenced by insecticide application. Among the predators found in the plots, Doru luteipes was not affected by spraying, whereas the abundance of Orius sp. decreased 21 days after insecticide application. The frequency of the ant Pheidole sp. was not affected by chlorpyrifos; however, foraging activity of Crematogaster sp. on treated plots was rapidly reduced and remained low during the following 2 weeks. The small number of damaged ears suggests that the insecticide did not affect the efficiency of the natural enemies of H . zea . |
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Thesagro: |
Milho; Praga de planta. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 01865naa a2200193 a 4500
001 1486558
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aFILHO, M. M.
245 $aChlorpyrifos sprying of no-tillage corn during tasselling and its effect on damage by Helicoverpa zea (Lep., Noctuidae) and on its natural enemies.$h[electronic resource]
260 $c2002
520 $aCorn plants were twice sprayed at 3-day intervals during tasselling to evaluate the effect of chlorpyrifos on ear damage by H . zea and also on the populations of the natural enemies associated with this moth. The abundance of H . zea eggs and larvae and of predators on the whole plant, the egg parasitism by Trichogramma pretiosum Riley and the percentage of injured ears were sampled weekly. Chlorpyrifos did not show any significant effect on population levels and on ear damage by H . zea . Natural parasitism of eggs ranged from 52 to 75% and did not differ between treated and untreated plots; furthermore, the percentage of non viable eggs and adult emergence were not influenced by insecticide application. Among the predators found in the plots, Doru luteipes was not affected by spraying, whereas the abundance of Orius sp. decreased 21 days after insecticide application. The frequency of the ant Pheidole sp. was not affected by chlorpyrifos; however, foraging activity of Crematogaster sp. on treated plots was rapidly reduced and remained low during the following 2 weeks. The small number of damaged ears suggests that the insecticide did not affect the efficiency of the natural enemies of H . zea .
650 $aMilho
650 $aPraga de planta
700 1 $aDELLA LUCIA, T. M. C.
700 1 $aCRUZ, I.
700 1 $aGUEDES, R. N. C.
700 1 $aGALVAO, J. C. C.
773 $tJournal of Applied Entomology, Hamburg$gv. 126, n. 7/8, p. 422-430, 2002.
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Embrapa Milho e Sorgo (CNPMS) |
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