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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
24/02/2000 |
Data da última atualização: |
22/09/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LOPES. M. A. |
Afiliação: |
Embrapa Milho e Sorgo. |
Título: |
Identificação de demandas de pesquisa tecnológica para expansão da cultura do milheto no Brasil. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: WORKSHOP INTERNACIONAL DE MILHETO, 1999, Planaltina, DF. Anais... Planaltina: Embrapa Cerrados, 1999. p. 199 |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Demand; Identification. |
Thesagro: |
Demanda; Identificação; Milheto; Pennisetum Glaucum; Pesquisa. |
Thesaurus Nal: |
Brazil; millets; research. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/73808/1/Identificacao-demandas.pdf
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Marc: |
LEADER 00715nam a2200229 a 4500 001 1483404 005 2015-09-22 008 1999 bl uuuu u00u1 u #d 100 1 $aLOPES. M. A. 245 $aIdentificação de demandas de pesquisa tecnológica para expansão da cultura do milheto no Brasil.$h[electronic resource] 260 $aIn: WORKSHOP INTERNACIONAL DE MILHETO, 1999, Planaltina, DF. Anais... Planaltina: Embrapa Cerrados, 1999. p. 199$c1999 650 $aBrazil 650 $amillets 650 $aresearch 650 $aDemanda 650 $aIdentificação 650 $aMilheto 650 $aPennisetum Glaucum 650 $aPesquisa 653 $aBrasil 653 $aDemand 653 $aIdentification
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
14/01/2011 |
Data da última atualização: |
17/04/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. |
Afiliação: |
ÉRICO A. R. VASCONCELOS, UNB; CELSO G. SANTANA; CLAUDIA VIEIRA GODOY, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO X. F. FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010. |
Idioma: |
Inglês |
Conteúdo: |
Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. |
Palavras-Chave: |
Asian rust. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31109/1/cbb.errata.cvg.pdf
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Marc: |
LEADER 02303nam a2200253 a 4500 001 1873147 005 2018-04-17 008 2010 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, E. A. R. 245 $aA new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. 260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec$c2010 520 $aAsian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. 653 $aAsian rust 700 1 $aSANTANA, C. G. 700 1 $aGODOY, C. V. 700 1 $aSILVA, M. S. 700 1 $aMOREIRA, L. R. S. 700 1 $aOLIVEIRA-NETO, OSMUNDO B. 700 1 $aPRICE, D. 700 1 $aFITCHES, E. 700 1 $aFILHO, E. X. F. 700 1 $aMEHTA, A. 700 1 $aGATEHOUSE, J. A. 700 1 $aGROSSI-DE-SA, M. F.
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Embrapa Soja (CNPSO) |
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