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| Registros recuperados : 383 | |
| 301. |  | MIRANDA, V. J.; BASSO, A. M. M.; OLIVEIRA, R. S.; OLIVEIRA NETO, O. B.; EVANGELISTA, I. S.; PAULA, A. W. M.; COSTA, P. H. A.; LOURENÇO, I. T.; SILVA, M. C. M.; GROSSI DE SÁ, M. F. Genetic transformation of cotton plants for resistance to Coleoptera insect-pest. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 302. | | MOURA, S. M. de; ARTICO, S.; LIMA, C.; NARDELI, S. M.; BERBEL, A.; OLIVEIRA-NETO, O. B.; GROSSI-DE-SA, M. F.; FERRÁNDIZ, C.; MADUEÑO, F.; ALVES-FERREIRA, M. Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones. Plant Reproduction, v. 30, n. 1, p. 19-40, 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 303. | | DIAS, S. C.; SILVA, M. M. da; OLIVEIRA NETO, O. B. de; MAGALHÃES, C. P.; TEIXEIRA, F. R.; FRANCO, O. L.; FILGUEIRA, E. L. Z.; LAUMANN, R.; MELLO, F.; GROSSI-de-SÁ, M. F. Functional expression of a a- amylase/trypsin inhibitor domain from rye and its potential use in the control of cotton boll wevil (Anthonomus grandis). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 20., 2004, Gramado. Programa e resumos... Gramado: Sociedade Entomológica do Brasil, 2004. p. 261.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 304. | | SILVA, D. P.; CASADO-FILHO, E. L.; CORRÊA, A. S. R.; FARIAS, L. R.; BLOCH JUNIOR, C.; GROSSI DE SÁ, M. F.; MENDES, P. A. M.; QUIRINO, B. F.; NORONHA, E. F.; FRANCO, O. L. Identification of an alpha-amylase inhibitor from Pterodon pubescens with ability to inhibit Cowpea weevil digestive enzymes. Journal Agricultural and Food Chemistry, v. 55, p. 4382-4387, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 305. | | GROSSI DE SA, M. F.; SOUZA, D. S. L.; ROMANO, E.; SOUSA, B. A. de; GROSSI DE SA, M.; PIRES, N.; ROCHA, T. L.; BARBOSA, A. E. A. D.; SA, C. M.; CARNEIRO, R.; DIAS, S. C.; TEIXEIRA, F. M. High level of resistance to Meloidogyne incognita in transgenic Nicotiana tobacum plants by knockdown of essential oesophagial pionner genes using RNAI. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 13., 2007, Sorrento. Book of abstracts... Sorrento: [s. n.], 2007. p.197.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 306. | | BASSO, M. F.; LOURENCO-TESSUTTI, I. T.; MENDES, R. A. G.; PINTO, C. E. M.; BOURNAUD, C.; GILLET, F.-X.; TOGAWA, R. C.; MACEDO, L. L. P. de; ENGLER, J. d A.; GROSSI-DE-SA, M. F. MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita. Scientific Reports, v. 10, n. 1, p. 1-13, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 307. | | LACERDA, A. F.; BENEVENTI, M. A.; DEL SARTO, R. P.; CRUZ, C. C. M.; OLIVEIRA, G. R.; SILVA, M. S. S.; ROCHA, T. L.; MATTAR, M. C. S.; GROSSI DE SÁ, M. F. Methylotrophic yeast expression of sunflower defensin (SD2). In: ANNUAL MEETING OF SBBQ, 27.; CONGRESS OF THE PAN-AMERICAN ASSOCIATION FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 11., 2008, Águas de Lindóia. Program and index... Águas de Lindóia: SBBq, 2008. 37º Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular.
Resumo - R8934.| Biblioteca(s): Embrapa Cerrados. |
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| 308. | | LACERDA, A. F.; BENEVENTI, M. A.; DEL SARTO, R. P.; CRUZ, C. C. M.; OLIVEIRA, G. R.; SILVA, M. S. S.; ROCHA, T. L.; MATTAR, M. C. S.; GROSSI DE SÁ, M. F. Methylotrophic yeast expression of sunflower defensin (SD2). In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 309. | | LUCENA, W. A.; PELEGRINI, P. B.; MARTINS-DE-SA, D.; FONSECA, F. C. A.; GOMES JÚNIOR, J. E.; MACEDO, L. L. P. de; SILVA, M. C. M. da; OLIVEIRA, R. D.; GROSSI DE SA, M. F. Molecular approaches to improve the insecticidal activity of Bacillus thuringiensis cry toxins. Toxins, v. 6, p. 2393-2423, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 310. | | OLIVEIRA-NETO, O.B.; BATISTA, J. A. N.; RIGDEN, D. J.; FRANCO, O. L.; FALÇÃO, R.; FRAGOSO, R. R.; MELLO, L. V.; SANTOS, R. C. dos; GROSSI-DE-SÁ, M.F. Molecular claning of x-amylases from cotton Bollweevil, Anterononhus grandis and tructural relations to plant inhibitores: an approach to insect resistance. Journal of Porotein Chemistry, v.22, n.1, p.77-87, 2003.| Biblioteca(s): Embrapa Algodão. |
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| 311. | | OLIVEIRA NETO, O. B. de; BATISTA, J. A. N.; RIGDEN, D. J.; FRANCO, O. L.; FRAGOSO, R. R.; MONTEIRO, A. C. S.; MONNERAT, R. G.; GROSSI-DE-SÁ, M. F. Molecular cloning of a cysteine proteinase cDNA from the cotton boll weevil Anthonomus grandis (Coleoptera: Curculionidae). Bioscience, Biotechnology and Biochemistry, v. 68, n. 6, p. 1235-1242, 2004.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 312. | | DIAS, S. C.; FRANCO, O. L.; MAGALHÃES, C. P.; OLIVEIRA-NETO, O. B. de; ALUMANN, R. A.; FIGUEIRA, E. L. Z.; MELO, F. R.; GROSSI-de-SÁ, M. F. Molecular cloning and expression of an alpha- amylase inhibitor from rye with potential for controlling insect pests. The Protein Journal, v. 24, n. 2, p. 113-123, Feb. 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 313. | | GROSSI-de-SÁ, M. F.; FIGUEIRA, E. L. Z.; DEL SARTO, R. P.; OLIVEIRA NETO, O. B.; OLIVEIRA, G. R.; BRUNETTA, P. S. F.; ROMANO, E.; SILVA, M. C. M. Molecular evolution of alpha-amylase inhibitor: screening for cotton boll weevil, Anthonomus grandis. The FEBS Journal, v. 271, p. 511-512, 2005. Supplement 1. Edition of the Abstracts of 30th FEBS Congress and 9th IUBMB Conference, Budapest, Hungary, 2-7 July 2005. The protein world.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 314. | | BONAVIDES, K. B.; PELEGRINI, P. B.; LAUMANN, R. A.; GROSSI DE SÁ, M. F.; BLOCH JÚNIOR, C.; MELO J. A. T.; QUIRINO, B. F.; NORONHA, E. F.; FRANCO, O. L. Molecular identification of four different alfa-amylase inhibitors from Baru (Dipteryx alata) seeds with activity toward insect enzymes. Journal of Biochemistry and Molecular Biology, v. 40, n. 4, p. 494-500, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 315. | | AGUIAR, J. M.; FRANCO, O. L.; RIGDEN, D. J.; BLOCH JUNIOR, C.; MONTEIRO, A. C. S.; FLORES, V. M. Q.; JACINTO, T.; XAVIER-FILHO, J.; OLIVEIRA, A. E. A.; GROSSI-DE-SÁ, M. F.; FERNANDES, K. V. S. Molecular modeling and inhibitory activity of cowpea cystatin against bean bruchid pests. Proteins: structure, function, and bioinformatics, v. 63, p. 662-670, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 316. | | MAGALHÃES, C. P.; FRAGOSO, R. R.; SOUZA, D. S. L.; BARBOSA, A. E. A. D.; SILVA, C. P.; FINARDI FILHO, F.; SILVA, C. M. da; ROCHA, T. L.; FRANCO, O. L.; GROSSI DE SA, M. F. Molecular and structural characterization of a trypsin highly expressed in larval stage of Zabrotes subfasciatus. Archives of Insect Biochemistry and Physiology, v. 66, p. 169-182, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 317. | | MAGALHÃES, C. P.; FRAGOSO, R. R.; SOUZA, D. S. L.; BARBOSA, A. E. A. D.; SILVA, C. P.; FINARDI FILHO, F.; SILVA, M. C. M. da; ROCHA, T. L.; FRANCO, O. L.; GROSSI-DE-SA, M. F. Molecular and structural characterization of a trypsin highly expressed in larval stage of Zabrotes subfasciatus. Archives of Insect Biochemistry and Physiology, New York, v. 66, p. 169-182, 2007.| Biblioteca(s): Embrapa Cerrados. |
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| 318. | | CAMPOS, M. L.; PRADO, G. S.; SANTOS, V. O. dos; NASCIMENTO, L. C.; DOHMS, S. M.; CUNHA, N. B. da; RAMADA, M. H. S.; GROSSI-DE-SA, M. F.; DIAS, S. C. Mosses: versatile plants for biotechnological applications. Biotechnology Advances, v. 41, article 107533, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 319. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010.| Biblioteca(s): Embrapa Soja. |
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| 320. | | FRAGOSO, R. da R.; LOURENÇO, I. T.; BATISTA, J. A. N.; OLIVEIRA NETO, O. B.; SILVA, M. C. M.; ROCHA, T. L.; COUTINHO, M. V.; GROSSI DE SÁ, M. F. Meloidogyne incognita: molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase. Experimental Parasitology, v. 121, p. 115-123, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 383 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/09/2020 |
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Data da última atualização: |
17/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. |
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Afiliação: |
BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 11, article 1228, 2020. |
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DOI: |
https://doi.org/10.3389/fpls.2020.01228 |
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Idioma: |
Inglês |
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Conteúdo: |
The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs. MenosThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for stud... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja. |
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Thesagro: |
Cultura de Tecido; Glycine Max; Soja. |
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Thesaurus NAL: |
Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf
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Marc: |
LEADER 02941naa a2200397 a 4500
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008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fpls.2020.01228$2DOI
100 1 $aMELO, B. P. de
245 $aSoybean embryonic axis transformation$bcombining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.$h[electronic resource]
260 $c2020
520 $aThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.
650 $aAgrobacterium
650 $aEmbryonic structures
650 $aGenetic transformation
650 $aPlant genetics
650 $aCultura de Tecido
650 $aGlycine Max
650 $aSoja
653 $aAgrobacterium-mediated transformation
653 $aEmbryonic axis
653 $aHigh-efficiency plant transformation
653 $aParticle bombardment
653 $aPlanta geneticamente modificada
653 $aRecuperação trangênica de soja
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, N. C.
700 1 $aPINHEIRO, L. B.
700 1 $aLINS, C. B. de J.
700 1 $aSILVA, M. C. M.
700 1 $aMACEDO, L. L. P.
700 1 $aFONTES, E. P. B.
700 1 $aGROSSI-DE-SA, M. F.
773 $tFrontiers in Plant Science$gv. 11, article 1228, 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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