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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
Data corrente: |
14/12/2020 |
Data da última atualização: |
14/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FIGUEIRO, M. R.; GARCIA, J. M.; LIMA, M. R. de; COLLADO, M. del; SARAIVA, N. Z. |
Afiliação: |
MARIVALDO RODRIGUES FIGUEIRO, CPATU; Joaquim Mansano Garcia, UNESP; Marina Ragagnin de Lima, UNESP; Maite del Collado, USP; NAIARA ZOCCAL SARAIVA, CNPGL. |
Título: |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes? |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Zygote, v. 28, n. 2, p. 109-115, Apr. 2020. |
DOI: |
https://doi.org/10.1017/S096719941900073X |
Idioma: |
Inglês |
Conteúdo: |
In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. MenosIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 ... Mostrar Tudo |
Palavras-Chave: |
Desenvolvimento embrionário; Soro fetal bovino. |
Thesagro: |
Búfalo; Lipídio. |
Thesaurus Nal: |
Buffaloes; Lipid metabolism. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02374naa a2200253 a 4500 001 2128094 005 2020-12-14 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S096719941900073X$2DOI 100 1 $aFIGUEIRO, M. R. 245 $aIs it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?$h[electronic resource] 260 $c2020 520 $aIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. 650 $aBuffaloes 650 $aLipid metabolism 650 $aBúfalo 650 $aLipídio 653 $aDesenvolvimento embrionário 653 $aSoro fetal bovino 700 1 $aGARCIA, J. M. 700 1 $aLIMA, M. R. de 700 1 $aCOLLADO, M. del 700 1 $aSARAIVA, N. Z. 773 $tZygote$gv. 28, n. 2, p. 109-115, Apr. 2020.
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Embrapa Amazônia Oriental (CPATU) |
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Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
25/10/2004 |
Data da última atualização: |
21/07/2014 |
Tipo da produção científica: |
Documentos |
Autoria: |
MEDRADO, M. J. S.; GRIGOLETTI JUNIOR, A.; STURION, J. A.; CORRÊA, G.; SOARES, C. M. de S.; MOSELE, S. H. |
Afiliação: |
Medrado, Grigoletti Junior, Sturion, pesquisadores da Embrapa Florestas. |
Título: |
Proposta de modelo de organização da pesquisa agrícola no setor ervateiro do Brasil. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Colombo: Embrapa Florestas, 2004. |
Descrição Física: |
1 CD-ROM. |
Série: |
(Embrapa Florestas. Documentos, 95). |
ISSN: |
1679-2599 |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Erva mate; Indústria ervateira; Organização; Setor ervateiro. |
Thesagro: |
Espécie nativa; Pesquisa; Planejamento; Política. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPF-2009-09/35642/1/doc95.pdf
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Marc: |
LEADER 00837nam a2200301 a 4500 001 1309710 005 2014-07-21 008 2004 bl uuuu u0uu1 u #d 022 $a1679-2599 100 1 $aMEDRADO, M. J. S. 245 $aProposta de modelo de organização da pesquisa agrícola no setor ervateiro do Brasil. 260 $aColombo: Embrapa Florestas$c2004 300 $c1 CD-ROM. 490 $a(Embrapa Florestas. Documentos, 95). 650 $aEspécie nativa 650 $aPesquisa 650 $aPlanejamento 650 $aPolítica 653 $aBrasil 653 $aErva mate 653 $aIndústria ervateira 653 $aOrganização 653 $aSetor ervateiro 700 1 $aGRIGOLETTI JUNIOR, A. 700 1 $aSTURION, J. A. 700 1 $aCORRÊA, G. 700 1 $aSOARES, C. M. de S. 700 1 $aMOSELE, S. H.
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