Data/hora: 24/06/2024 21:55:14

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	31/05/2007	
Data da última atualização: 	08/07/2019	
Autoria: 	ESTRADA-NAVARRETE, G.; ALVARADO-AFFANTRANGER, X.; OLIVARES, J.-E.; DÍAZ-CAMINO, C.; SANTANA, O.; MURILLO, E.; GUILLÉN, G.; SÁNCHEZ-GUEVARA, N.; ACOSTA, J.; QUINTO, C.; LI, D.; GRESSHOFF, P. M.; SÁNCHEZ, F.	
Título: 	Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics.	
Ano de publicação: 	2006	
Fonte/Imprenta: 	Molecular Plant-Microbe Interactions, St. Paul, v. 19, n. 12, p. 1385-1393, 2006.	
Idioma: 	Inglês	
Conteúdo: 	A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes  was developed for Phaseolus vulgaris L. wild accessions, land races, and culti-vars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. In-duced hairy roots are robust and grow quickly. The trans-formation frequency is between 75 and 90% based on the 35.S promoter-driven green fluorescent protein and f)-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal de-terminate nodules that fix nitrogen as etliciently as inocu-lated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiol-ogy, root metabolism, and root-microbe interactions.	
Thesaurus Nal: 	Agrobacterium.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (34163-1) - UPC - SP - -- -  -  - 0023205 - DIS