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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/01/2009 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
WOLFF, J. L.; VALICENTE, F. H.; MARTINS, R.; OLIVEIRA, J. V. de C.; ZANOTTO, P. M. de A. |
Afiliação: |
José Luiz Caldas Wolff, Universidade de Mogi das Cruzes; FERNANDO HERCOS VALICENTE, CNPMS; Renata Martins, Universidade de Mogi das Cruzes; Juliana Velasco de Castro Oliveira, USP; Paolo Marinho de andrade Zanotto, USP. |
Título: |
Analysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Journal of General Virology, Cambridge, v. 89, n. 6, p. 1202-1211, 2008. |
DOI: |
10.1099/vir.0.83581-0 |
Idioma: |
Inglês |
Conteúdo: |
The genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative of temporal organization in the gene accretion processo. MenosThe genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative ... Mostrar Tudo |
Thesagro: |
Praga de planta. |
Categoria do assunto: |
O Insetos e Entomologia |
Marc: |
LEADER 02240naa a2200193 a 4500 001 1491654 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1099/vir.0.83581-0$2DOI 100 1 $aWOLFF, J. L. 245 $aAnalysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses.$h[electronic resource] 260 $c2008 520 $aThe genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative of temporal organization in the gene accretion processo. 650 $aPraga de planta 700 1 $aVALICENTE, F. H. 700 1 $aMARTINS, R. 700 1 $aOLIVEIRA, J. V. de C. 700 1 $aZANOTTO, P. M. de A. 773 $tJournal of General Virology, Cambridge$gv. 89, n. 6, p. 1202-1211, 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
28/08/2023 |
Data da última atualização: |
27/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
VIEIRA FILHO, J. S. M.; GUERRA, J. G. M.; GOULART, J. M.; ARAUJO, E. da S.; ESPINDOLA, J. A. A.; ROUWS, J. R. C. |
Afiliação: |
JOSÉ SÁVIO M VIEIRA FILHO, UFRRJ; JOSE GUILHERME MARINHO GUERRA, CNPAB; JHONATAN M GOULART, UFRRJ; EDNALDO DA SILVA ARAUJO, CNPAB; JOSE ANTONIO AZEVEDO ESPINDOLA, CNPAB; JANAINA RIBEIRO COSTA ROUWS, CNPAB. |
Título: |
Management of green manure and organic fertilization with fermented compost fertilizer in agroecological cultivation of American broccoli |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Horticultura Brasileira, v. 41, e2599, 2023. |
ISSN: |
1806-9991 |
DOI: |
https://doi.org/10.1590/s0102-0536-2023-e2599 |
Idioma: |
Inglês |
Conteúdo: |
his work aimed to evaluate the phytotechnical performance of the succession involving pre-croppings of maize and Mucuna pruriens (velvet bean) or the intercropping of these species in the agroecological cultivation of broccoli in the presence and absence of fermented compost fertilization. The experimental design was in randomized blocks in a factorial scheme 2 (maize monoculture or intercropped with velvet bean) x 2 (absence and presence of fermented compost fertilization) + 1 (velvet bean monoculture and without fertilization). After cutting the maize and velvet bean biomass, the broccoli seedlings were transplanted. In this stage, the design was in randomized blocks in a 3-factorial scheme (maize monoculture; maize intercropped with velvet bean; velvet bean monoculture) x 2 (presence and absence of fermented compost fertilization). The authors concluded that when the velvet bean was introduced into maize cultivation system no negatively interference was noticed concerning to phytotechnical components of baby corn, and it even allows an additional of 3.46 and 7.75 t/ha of fabaceous phytomass, respectively, in the first and second year. In the absence of fertilization, phytomasses of the intercroppings provided broccoli productivity of 12.87 t/ha, which represents an increase of 43.73% when compared to maize monoculture in the second year. An effect of fertilization with the fermented compost on broccoli cultivation under these study conditions was verified, providing a greater accumulation of N in the inflorescences in the first year and maximum productivity of 12.19 and 14.32 t/ha, respectively, first and second years. Menoshis work aimed to evaluate the phytotechnical performance of the succession involving pre-croppings of maize and Mucuna pruriens (velvet bean) or the intercropping of these species in the agroecological cultivation of broccoli in the presence and absence of fermented compost fertilization. The experimental design was in randomized blocks in a factorial scheme 2 (maize monoculture or intercropped with velvet bean) x 2 (absence and presence of fermented compost fertilization) + 1 (velvet bean monoculture and without fertilization). After cutting the maize and velvet bean biomass, the broccoli seedlings were transplanted. In this stage, the design was in randomized blocks in a 3-factorial scheme (maize monoculture; maize intercropped with velvet bean; velvet bean monoculture) x 2 (presence and absence of fermented compost fertilization). The authors concluded that when the velvet bean was introduced into maize cultivation system no negatively interference was noticed concerning to phytotechnical components of baby corn, and it even allows an additional of 3.46 and 7.75 t/ha of fabaceous phytomass, respectively, in the first and second year. In the absence of fertilization, phytomasses of the intercroppings provided broccoli productivity of 12.87 t/ha, which represents an increase of 43.73% when compared to maize monoculture in the second year. An effect of fertilization with the fermented compost on broccoli cultivation under these study conditions was verified, providing a gre... Mostrar Tudo |
Palavras-Chave: |
Bokashi; Organic agriculture. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02404naa a2200229 a 4500 001 2156160 005 2023-09-27 008 2023 bl uuuu u00u1 u #d 022 $a1806-9991 024 7 $ahttps://doi.org/10.1590/s0102-0536-2023-e2599$2DOI 100 1 $aVIEIRA FILHO, J. S. M. 245 $aManagement of green manure and organic fertilization with fermented compost fertilizer in agroecological cultivation of American broccoli$h[electronic resource] 260 $c2023 520 $ahis work aimed to evaluate the phytotechnical performance of the succession involving pre-croppings of maize and Mucuna pruriens (velvet bean) or the intercropping of these species in the agroecological cultivation of broccoli in the presence and absence of fermented compost fertilization. The experimental design was in randomized blocks in a factorial scheme 2 (maize monoculture or intercropped with velvet bean) x 2 (absence and presence of fermented compost fertilization) + 1 (velvet bean monoculture and without fertilization). After cutting the maize and velvet bean biomass, the broccoli seedlings were transplanted. In this stage, the design was in randomized blocks in a 3-factorial scheme (maize monoculture; maize intercropped with velvet bean; velvet bean monoculture) x 2 (presence and absence of fermented compost fertilization). The authors concluded that when the velvet bean was introduced into maize cultivation system no negatively interference was noticed concerning to phytotechnical components of baby corn, and it even allows an additional of 3.46 and 7.75 t/ha of fabaceous phytomass, respectively, in the first and second year. In the absence of fertilization, phytomasses of the intercroppings provided broccoli productivity of 12.87 t/ha, which represents an increase of 43.73% when compared to maize monoculture in the second year. An effect of fertilization with the fermented compost on broccoli cultivation under these study conditions was verified, providing a greater accumulation of N in the inflorescences in the first year and maximum productivity of 12.19 and 14.32 t/ha, respectively, first and second years. 653 $aBokashi 653 $aOrganic agriculture 700 1 $aGUERRA, J. G. M. 700 1 $aGOULART, J. M. 700 1 $aARAUJO, E. da S. 700 1 $aESPINDOLA, J. A. A. 700 1 $aROUWS, J. R. C. 773 $tHorticultura Brasileira$gv. 41, e2599, 2023.
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