Data/hora: 24/06/2024 21:49:41

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 18/01/2023
Data da última atualização: | 25/10/2023
Autoria: | ALMEIDA, J. R. M. de; WIMAN, M.; HEER, D.; BRINK, D. P.; SAUER, U.; HAHN‐HÄGERDAL, B.; LIDÉN, G.; GORWA‐GRAUSLUND, M. F.
Afiliação: | JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; MAGNUS WIMAN, LUND UNIVERSITY; DOMINIK HEER, INSTITUTE OF MOLECULAR SYSTEMS BIOLOGY; DANIEL P. BRINK, LUND UNIVERSITY; UWE SAUER, INSTITUTE OF MOLECULAR SYSTEMS BIOLOGY; BÄRBEL HAHN‐HÄGERDAL, LUND UNIVERSITY; GUNNAR LIDÉN, LUND UNIVERSITY; MARIE F. GORWA‐GRAUSLUND, LUND UNIVERSITY.
Título: | Physiological and molecular characterization of yeast cultures pre-adapted for fermentation of lignocellulosic hydrolysate.
Ano de publicação: | 2023
Fonte/Imprenta: | Fermentation, v. 9, n. 72, p. 2-21, 2023.
DOI: | https://doi.org/10.3390/fermentation9010072
Idioma: | Inglês
Conteúdo: | Economically feasible bioethanol process from lignocellulose requires efficient fermentation by yeast of all sugars present in the hydrolysate. However, when exposed to lignocellulosic hydrolysate, Saccharomyces cerevisiae is challenged with a variety of inhibitors that reduce yeast viability, growth, and fermentation rate, and in addition damage cellular structures. In order to evaluate the capability of S. cerevisiae to adapt and respond to lignocellulosic hydrolysates, the physiological effect of cultivating yeast in the spruce hydrolysate was comprehensively studied by assessment of yeast performance in simultaneous saccharification and fermentation (SSF), measurement of furaldehyde reduction activity, assessment of conversion of phenolic compounds and genome&#8208;wide transcription analysis. The yeast cultivated in spruce hydrolysate developed a rapid adaptive response to lignocellulosic hydrolysate, which significantly improved its fermentation performance in subsequent SSF experiments. The adaptation was shown to involve the induction of NADPHdependent aldehyde reductases and conversion of phenolic compounds during the fed&#8208;batch cultivation. These properties were correlated to the expression of several genes encoding oxidoreductases, notably AAD4, ADH6, OYE2/3, and YML131w. The other most significant transcriptional changes involved genes involved in transport mechanisms, such as YHK8, FLR1, or ATR1. A large set of genes were found to be associated with transcription factors (TFs) involved in stress response (Msn2p, Msn4p, Yap1p) but also cell growth and division (Gcr4p, Ste12p, Sok2p), and these TFs were most likely controlling the response at the post&#8208;transcriptional level.
Thesaurus Nal: | Industrial microbiology; Lignocellulose; Microarray technology; Phenolic compounds; Transcriptomics.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151045/1/Physiological-and-molecular.pdf
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (4168-1) - UPC - AP - DD -  -  -  - DIS