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| Registros recuperados : 28 | |
| Registros recuperados : 28 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/01/2023 |
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Data da última atualização: |
09/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BOLÍVAR-GONZÁLEZ, L.; MOLINA-BRAVO, R.; SOLANO-SÁNCHEZ, W.; ARAYA-VALVERDE, E.; IVAMOTO-SUZUKI, S. T.; PEREIRA, L. F. P.; GATICA-ARIAS, A. |
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Afiliação: |
ALEJANDRO BOLÍVAR-GONZÁLEZ, UNIVERSIDAD DE COSTA RICA; RAMÓN MOLINA-BRAVO, UNIVERSIDAD NACIONAL; WILLIAM SOLANO-SÁNCHEZ, CENTRO AGRONÓMICO TROPICAL DE INVESTIGACIÓN Y ENSEÑANZA; EMANUEL ARAYA-VALVERDE, CENTRO NACIONAL DE INNOVACIONES BIOTECNOLÓGICAS; SUZANA T. IVAMOTO-SUZUKI, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; ANDRÉS GATICA-ARIAS, UNIVERSIDAD DE COSTA RICA. |
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Título: |
SNP markers found in non-coding regions can distinguish among low-variant genotypes of arabica and other coffee species. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, Dec. 2022. |
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DOI: |
https://doi.org/10.1007/s10722-022-01498-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Development of efficient and scalable methods for molecular identification of Coffea spp. are necessary to accelerate studies related to the characterization of germplasm for both conservation or breeding purposes, and the validation of coffee germplasm. The low genetic diversity of coffee hinders the establishment of protocols that facilitate the molecular characterization of a given genotype. In this study, nucleotide variability was analyzed at 22 loci in the genome of 19 coffee accessions using de novo primer sets and high-resolution melting (HRM). Single nucleotide polymorphisms (SNPs) variants were studied in coding regions of genes implicated in sucrose accumulation in the seed, Sucrose synthase 2 (SUS2), Ent-kaurene oxidase 1 (CaKO1), and Caffeoyl-coenzyme A 3-O-methyltransferase (CcOAOMT). The non-coding Internal transcribed spacer 2 (ITS2) region was also studied. Variability was shown at 103 positions both at the interspecies level (15 loci) and among varieties of Coffea arabica L. (4 loci). The HMR technique for identification of variants in genes CaKO1, SUS2, CcoAOMT, as well as in the ITS2 region proved to be a robust technique for germplasm characterization. More important this technique can be used for fingerprinting and traceability of coffee grain exports which is an increasing market-consumer demand. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus NAL: |
Genotype; Single nucleotide polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150820/1/SNP-markers-found-innon.pdf
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Marc: |
LEADER 02141naa a2200241 a 4500
001 2150820
005 2023-01-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10722-022-01498-0$2DOI
100 1 $aBOLÍVAR-GONZÁLEZ, L.
245 $aSNP markers found in non-coding regions can distinguish among low-variant genotypes of arabica and other coffee species.$h[electronic resource]
260 $c2022
520 $aDevelopment of efficient and scalable methods for molecular identification of Coffea spp. are necessary to accelerate studies related to the characterization of germplasm for both conservation or breeding purposes, and the validation of coffee germplasm. The low genetic diversity of coffee hinders the establishment of protocols that facilitate the molecular characterization of a given genotype. In this study, nucleotide variability was analyzed at 22 loci in the genome of 19 coffee accessions using de novo primer sets and high-resolution melting (HRM). Single nucleotide polymorphisms (SNPs) variants were studied in coding regions of genes implicated in sucrose accumulation in the seed, Sucrose synthase 2 (SUS2), Ent-kaurene oxidase 1 (CaKO1), and Caffeoyl-coenzyme A 3-O-methyltransferase (CcOAOMT). The non-coding Internal transcribed spacer 2 (ITS2) region was also studied. Variability was shown at 103 positions both at the interspecies level (15 loci) and among varieties of Coffea arabica L. (4 loci). The HMR technique for identification of variants in genes CaKO1, SUS2, CcoAOMT, as well as in the ITS2 region proved to be a robust technique for germplasm characterization. More important this technique can be used for fingerprinting and traceability of coffee grain exports which is an increasing market-consumer demand.
650 $aGenotype
650 $aSingle nucleotide polymorphism
650 $aCoffea Arábica
700 1 $aMOLINA-BRAVO, R.
700 1 $aSOLANO-SÁNCHEZ, W.
700 1 $aARAYA-VALVERDE, E.
700 1 $aIVAMOTO-SUZUKI, S. T.
700 1 $aPEREIRA, L. F. P.
700 1 $aGATICA-ARIAS, A.
773 $tGenetic Resources and Crop Evolution, Dec. 2022.
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Registro original: |
Embrapa Café (CNPCa) |
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