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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
Data corrente: |
06/04/2021 |
Data da última atualização: |
29/04/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CHAVES, M. S.; AZEVEDO, H. C.; LUZ, V. B.; FERREIRA-SILVA, J. C.; BARROS, I. de; PAIVA, S. R.; MELO, E. de O.; PADILHA, D. de M. M.; FREITAS, V. J. de F.; BARTOLOMEU, C. C.; OLIVEIRA, M. A. L. |
Afiliação: |
MAIANA SILVA CHAVES, UFRPE; HYMERSON COSTA AZEVEDO, CPATC; VALESCA BARRETO LUZ, CESMAC; JOSÉ CARLOS FERREIRA-SILVA, UFRPE; INACIO DE BARROS, CNPGL; SAMUEL REZENDE PAIVA, Cenargen; EDUARDO DE OLIVEIRA MELO, Cenargen; DEBORAH DE MELO MAGALHÃES PADILHA, UNP; VICENTE JOSÉ DE FIGUEIRÊDO FREITAS, FACULTY OF VETERINARY, FORTALEZA; CLÁUDIO COUTINHO BARTOLOMEU, UFRPE; MARCOS ANTÔNIO LEMOS OLIVEIRA, UFRPE. |
Título: |
Occurrence, morphology, and morphometry of follicles containing multiple oocytes in FecGE mutant Santa Inês ewes. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Animal Reproduction Science, v. 226, 106690, 2021. |
ISSN: |
0378-4320 |
DOI: |
https://doi.org/10.1016/j.anireprosci.2021.106690 |
Idioma: |
Inglês |
Conteúdo: |
This study was conducted to characterize the morphology and morphometry of follicles containing multiple oocytes (MOFs) and determine the association with the FecGE mutation in Santa In?es ewes. Based on the genotypes, 65 ewes were characterized as being homozygous wild-type (n= 25; FecG+/+), heterozygous mutant (n = 27, FecG+/E), and homozygous mutant (n = 13, FecGE/E ). The variables evaluated were follicle developmental stage, number of oocytes per follicle, morphology, and morphometry of MOFs. The FecGE mutation did not affect the frequency of MOFs (P > 0.05) (3.0 % in FecG+/+; 3.3 % in FecG+/E ; and 3.5 % in FecGE/E). The greater viability (P < 0.05) of MOFs was identified in transitory stage of the FecGE/E (95.0 %) and FecG+/E (90.9 %) when compared to the FecG+/+ genotype (73.3 %). Furthermore, the morphology of transitory follicles with two oocytes was the variable and when evaluated was the most reliable determinant for predicting which ewes had an FecGE mutation. In conclusion, the FecGE mutation did not affect the frequency of MOFs. The ewes with FecGE mutation had a greater frequency of morphologically normal MOFs in the transitory stage. Furthermore, the ewes with the FecGE mutation had a greater likelihood of having MOFs containing two morphologically normal oocytes. |
Palavras-Chave: |
GDF-9; Multioócito; Multioocyte; Ovine; Prolificacy; Prolificidade. |
Thesagro: |
Mutação; Ovelha; Ovino. |
Thesaurus Nal: |
Mutation. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02397naa a2200385 a 4500 001 2132554 005 2022-04-29 008 2021 bl uuuu u00u1 u #d 022 $a0378-4320 024 7 $ahttps://doi.org/10.1016/j.anireprosci.2021.106690$2DOI 100 1 $aCHAVES, M. S. 245 $aOccurrence, morphology, and morphometry of follicles containing multiple oocytes in FecGE mutant Santa Inês ewes.$h[electronic resource] 260 $c2021 520 $aThis study was conducted to characterize the morphology and morphometry of follicles containing multiple oocytes (MOFs) and determine the association with the FecGE mutation in Santa In?es ewes. Based on the genotypes, 65 ewes were characterized as being homozygous wild-type (n= 25; FecG+/+), heterozygous mutant (n = 27, FecG+/E), and homozygous mutant (n = 13, FecGE/E ). The variables evaluated were follicle developmental stage, number of oocytes per follicle, morphology, and morphometry of MOFs. The FecGE mutation did not affect the frequency of MOFs (P > 0.05) (3.0 % in FecG+/+; 3.3 % in FecG+/E ; and 3.5 % in FecGE/E). The greater viability (P < 0.05) of MOFs was identified in transitory stage of the FecGE/E (95.0 %) and FecG+/E (90.9 %) when compared to the FecG+/+ genotype (73.3 %). Furthermore, the morphology of transitory follicles with two oocytes was the variable and when evaluated was the most reliable determinant for predicting which ewes had an FecGE mutation. In conclusion, the FecGE mutation did not affect the frequency of MOFs. The ewes with FecGE mutation had a greater frequency of morphologically normal MOFs in the transitory stage. Furthermore, the ewes with the FecGE mutation had a greater likelihood of having MOFs containing two morphologically normal oocytes. 650 $aMutation 650 $aMutação 650 $aOvelha 650 $aOvino 653 $aGDF-9 653 $aMultioócito 653 $aMultioocyte 653 $aOvine 653 $aProlificacy 653 $aProlificidade 700 1 $aAZEVEDO, H. C. 700 1 $aLUZ, V. B. 700 1 $aFERREIRA-SILVA, J. C. 700 1 $aBARROS, I. de 700 1 $aPAIVA, S. R. 700 1 $aMELO, E. de O. 700 1 $aPADILHA, D. de M. M. 700 1 $aFREITAS, V. J. de F. 700 1 $aBARTOLOMEU, C. C. 700 1 $aOLIVEIRA, M. A. L. 773 $tAnimal Reproduction Science$gv. 226, 106690, 2021.
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Embrapa Tabuleiros Costeiros (CPATC) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
Data corrente: |
23/05/2023 |
Data da última atualização: |
28/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
DIAS, J. H.; VERGANI; GONÇALVES, J. D.; OLIVEIRA, T. A.; PENITENTE-FILHO, J. M.; PEREIRA, V. S. do A.; ESTEVES, S. N.; GARCIA, A. R.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
Afiliação: |
J. H. DIAS, Universidade Federal de Viçosa; G. B. VERGANI, Universidade Estadual Paulista; J. D. GONÇALVES, Universidade Estadual Paulista; T. A. OLIVEIRA, Universidade Federal Fluminense; J. M. PENITENTE-FILHO, Universidade Federal de Viçosa; VERONICA SCHINAIDER DO A PEREIRA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ALEXANDRE ROSSETTO GARCIA, CPPSE; R. I. T. P. BATISTA, Universidade Federal Fluminense; M. E. F. OLIVEIRA, Universidade Estadual Paulista; J. M. G. SOUZA-FABJAN, Universidade Federal Fluminense; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
Different doses of pFSH are effective to promote follicular growth, superovulatory response, and embryo yield in White Dorper ewes. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Small Ruminant Research, v. 220, n. 103, mar. 2023, 106914. |
DOI: |
https://doi.org/10.1016/j.smallrumres.2023.106914 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study evaluated the effect of 250, 333, or 400 IU of a commercial porcine stimulating-follicle hormone (pFSH) on follicular growth, superovulatory (SOV) response, and embryo yield in White Dorper sheep. Ewes received an induced-ovulation protocol (D0 = progesterone (P4) device insertion) and superovulation starting 60 h before P4 withdrawal (D9), which consisted of six decreasing doses of 250 IU (G250; n = 15), 333 IU (G333; n = 15), or 400 IU (G400; n = 14) of pFSH. Ewes also received 50 µg of gonadotropin-releasing hormone (GnRH) analog on D10 and 300 IU of hCG on D13. Ewes were mated and submitted to non-surgical embryo recovery (NSER). Ultrasound evaluations were performed on D6, D7, D8, D9, and D16. The overall responding donor rate (≥3 CL) was 86.4% (38/44). The mean number of large follicles was higher (P < 0.05) in all groups on D8 (6.2 ± 0.9, 7.7 ± 0.9, and 8.3 ± 1.1) and D9 (11.1 ± 0.6, 10.4 ± 1.0, and 11.5 ± 0.9). Non-surgical embryo recovery procedures were successfully performed in 60.5% (23/38) of females. The CL count in successfully flushed ewes was greater (P < 0.05) in G333 (12.7 ± 2.3) and G400 (12.0 ± 1.3) than in G250 (6.0 ± 1.1). The recovery rate (44.4%, 57.3%, and 50.0%) and numbers of transferable embryos (2.3 ± 1.1, 6.1 ± 2.6, and 4.5 ± 1.3) did not differ (P > 0.05) among groups. All protocols were efficient in promoting follicular growth and adequate superovulatory response and embryo yield. |
Palavras-Chave: |
Follicular dynamics; FSH; MOET; Ovarian superestimulation; Ovine; PFSH. |
Thesaurus NAL: |
Ewes; Reproduction; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02594naa a2200373 a 4500 001 2153917 005 2023-11-28 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.smallrumres.2023.106914$2DOI 100 1 $aDIAS, J. H. 245 $aDifferent doses of pFSH are effective to promote follicular growth, superovulatory response, and embryo yield in White Dorper ewes.$h[electronic resource] 260 $c2023 520 $aAbstract: This study evaluated the effect of 250, 333, or 400 IU of a commercial porcine stimulating-follicle hormone (pFSH) on follicular growth, superovulatory (SOV) response, and embryo yield in White Dorper sheep. Ewes received an induced-ovulation protocol (D0 = progesterone (P4) device insertion) and superovulation starting 60 h before P4 withdrawal (D9), which consisted of six decreasing doses of 250 IU (G250; n = 15), 333 IU (G333; n = 15), or 400 IU (G400; n = 14) of pFSH. Ewes also received 50 µg of gonadotropin-releasing hormone (GnRH) analog on D10 and 300 IU of hCG on D13. Ewes were mated and submitted to non-surgical embryo recovery (NSER). Ultrasound evaluations were performed on D6, D7, D8, D9, and D16. The overall responding donor rate (≥3 CL) was 86.4% (38/44). The mean number of large follicles was higher (P < 0.05) in all groups on D8 (6.2 ± 0.9, 7.7 ± 0.9, and 8.3 ± 1.1) and D9 (11.1 ± 0.6, 10.4 ± 1.0, and 11.5 ± 0.9). Non-surgical embryo recovery procedures were successfully performed in 60.5% (23/38) of females. The CL count in successfully flushed ewes was greater (P < 0.05) in G333 (12.7 ± 2.3) and G400 (12.0 ± 1.3) than in G250 (6.0 ± 1.1). The recovery rate (44.4%, 57.3%, and 50.0%) and numbers of transferable embryos (2.3 ± 1.1, 6.1 ± 2.6, and 4.5 ± 1.3) did not differ (P > 0.05) among groups. All protocols were efficient in promoting follicular growth and adequate superovulatory response and embryo yield. 650 $aEwes 650 $aReproduction 650 $aSheep 653 $aFollicular dynamics 653 $aFSH 653 $aMOET 653 $aOvarian superestimulation 653 $aOvine 653 $aPFSH 700 1 $aVERGANI 700 1 $aGONÇALVES, J. D. 700 1 $aOLIVEIRA, T. A. 700 1 $aPENITENTE-FILHO, J. M. 700 1 $aPEREIRA, V. S. do A. 700 1 $aESTEVES, S. N. 700 1 $aGARCIA, A. R. 700 1 $aBATISTA, R. I. T. P. 700 1 $aOLIVEIRA, M. E. F. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aFONSECA, J. F. da 773 $tSmall Ruminant Research$gv. 220, n. 103, mar. 2023, 106914.
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