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| Registros recuperados : 37 | |
| 9. |  | AMORIM, C. A.; RODRIGUES, A. P. R.; LUCCI, C. M.; FIGUEIREDO, J. R.; GONÇALVES, P. B. D. Método mecânico para o isolamento de folículos pré-antrais a partir de ovários de ovelhas adultas. Arquivos da Faculdade de Veterinária UFRGS, v. 26, n. 1, p. 214, 1998. Suplemento. Resumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | MORAES, C. N.; OLIVEIRA, J. F. C.; SCHWEITZER, C. M.; GONÇALVES, P. B. D.; NEVES, J. P. Um método alternativo para congelamento de sêmen ovino em "pellets". In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 11., 1995, Belo Horizonte. Anais... Belo Horizonte, Colégio Brasileiro de Reprodução Animal, 1995. p. 313. Resumo 093.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | MORAES, C. N.; NEVES, J. P.; GONÇALVES, P. B. D.; SCHWEITZER, C. M.; LUZ, S. L. N. Eficácia do etileno glicol na criopreservação do sêmen ovino congelado em "pellets". In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 11., 1995, Belo Horizonte. Anais... Belo Horizonte, Colégio Brasileiro de Reprodução Animal, 1995. p. 312. Resumo 092.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | AMORIM, C. A.; LUCCI, C. M.; RODRIGUES, A. P.; CARVALHO, F. C. A.; FIGUEIREDO, J. R.; GONÇALVES, P. B. D. Eficiência do método específico para o isolamento de folículos pré-antrais a partir de ovários de ovelhas. Arquivos da Faculdade de Veterinária UFRGS, v. 26, n. 1, p. 210, 1998. Suplemento. Resumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | GASPERIN, B. G.; FRELING, G. F.; FERREIRA, R.; OLIVEIRA, J. F. C. de; BORDIGNON, V.; GONÇALVES, P. B. D. Intravaginal progestagen for estrus and parturition control in sows. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 8, p. 961-964, ago. 2011. Notas Científicas.
Título em português: Progestágeno intravaginal para controle do estro e do parto em fêmeas suínas.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 14. | | LIMA, A. K. F.; FIGUEIREDO, J. R.; OLIVEIRA, L. C.; GONCALVES, P. B. D.; BEZERRA, M. B. Isolamento enzimático de folículos ovarianos pré-antrais ovinos: resultados preliminares. Ciência Animal, v. 7, n. 2, p. 109, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | LUCCI, C. M.; AMORIM, C. A.; RODRIGUES, A. P. R.; FIGUEIREDO, J. R.; GONÇALVES, P. B. D. Isolamento mecânico de folículos pré-antrais a partir de ovários de cabras em diferentes estágios reprodutivos: resultados preliminares. Arquivos da Faculdade de Veterinária UFRGS, v. 26, n. 1, p. 304, 1998. Suplemento. Resumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | OLIVEIRA, L. C.; FIGUEIREDO, J. R.; LIMA, A. K. F.; GONÇALVES, P. B. D.; BEZERRA, M. B. Desenvolvimento de um método enzimático para o isolamento de folículos ovarianos pré-antrais caprinos. Ciência Animal, v. 7, n. 2, p. 116, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | CARAMBULA, S. F.; GONÇALVES, P. B. D.; FIGUEIREDO, J. R.; NEVES, J. P.; COSTA, L. F. S. Estudos preliminares sobre resgate de folículos pré-antrais de ovários de fetos ovinos. Arquivos da Faculdade de Veterinária UFRGS, v. 24, supl. p. 236, 1996. Edição dos anais da 11a. Reunião Anual da Sociedade Brasileira de Transferencia de Embrião, 1996, Cauela.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | BERTANI, G. R.; SCHEID, I. R.; FIALHO, F. B.; RUBIN, M. I. B.; WENTZ, IVO; GONCALVES, P. B. D. Periovulatory insemination with fresh or frozen semen on embryo viability and early pregnancy rate in gilts. In: INTERNATIONAL CONFERENCE ON BOAR SEMEN PRESERVATION, 3., 1995, Mariensee, Germany. Program and abstracts booklet. Mariensee: [s.n.], 1995. 2p.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 20. | | BERTANI, G. R.; SCHEID, I. R.; FIALHO, F. B.; RUBIN, M. I. B.; WENTZ, IVO; GONCALVES, P. B. D. Periovulatory insemination with fresh or frozen semen on embryo viability and early pregnancy rate in gilts. Reproduction in Domestic Animals, v.31, n.1, p.307-308, 1996.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 37 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
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Data corrente: |
14/06/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MORAES, F. P. de; D'AVILA, C. A.; OLIVEIRA, F. C. de; CASTRO, N. A. de; VIEIRA, A. D.; SCHNEIDER, A.; PFEIFER, L. F. M.; PEGORARO, L. M. C.; FERREIRA, R.; FERST, J. G.; ROVANI, M. T.; CORREA, M. N.; GONÇALVES, P. B. D.; LUCIA JUNIOR, T.; GASPERIN, B. G. |
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Afiliação: |
FABIANE PEREIRA DE MORAES, Federal University of Pelotas; CAMILA AMARAL D'AVILA, Federal University of Pelotas; FERNANDO CAETANO DE OLIVEIRA, Federal University of Pelotas; NATALIA ÁVILA DE CASTRO, Federal University of Pelotas; ARNALDO DINIZ VIEIRA, Federal University of Pelotas; AUGUSTO SCHNEIDER, Federal University of Pelotas; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; ROGERIO FERREIRA, Santa Catarina State University; JULIANA GERMANO FERST, Federal University of Santa Maria; MONIQUE TOMAZELE ROVANI, Federal University of Rio Grande do Sul; MARCIO NUNES CORREA, Federal University of Pelotas; PAULO BAYARD DIAS GONÇALVES, Federal University of Santa Maria / Federal University of Pampa; THOMAZ LUCIA JUINOR, Federal University of Pelotas; BERNARDO GARZIERA GASPERIN, Federal University of Pelotas. |
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Título: |
Prostaglandin F2a regulation and function during ovulation and luteinization in cows. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 171, p. 30-37, Sept. 2021. |
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ISSN: |
0093-691X |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2021.05.008 |
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Idioma: |
Inglês |
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Conteúdo: |
Although prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF group (P < 0.05), and no effect of the treatments on intrafollicular P4 and E2 concentrations 24 h after GnRH. The prostaglandin metabolite (PGFM) concentrations in the FF were greater in cows receiving systemic PGF (P < 0.001), which prompted us to further check its role on ovulation. Therefore, in Experiment 3, in a final attempt to demonstrate the local effect of PGF on ovulation, cows with preovulatory follicles received an intrafollicular injection (IFI) of PBS (Control) or 100 ng/mL purified PGF (PGF group). PGF treatment did not affect the time of ovulation after IFI (66 ± 6.4 and 63 ± 8.5 h for control and PGF, respectively; P > 0.05), further suggesting that it has no direct effect in the ovulatory process. Based on our findings, we concluded that FM decreased PGF synthesis within the follicle, whereas PGF treatment decreased follicular vascularization. In addition, the in vivo model of intrafollicular injection evidenced that PGF alone is not able to locally induce ovulation. MenosAlthough prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF ... Mostrar Tudo |
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Palavras-Chave: |
Ação anti-inflamatória; F2a; Luteinização. |
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Thesagro: |
Ovulação; Prostaglandina; Reprodução Animal; Vaca. |
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Thesaurus NAL: |
Animal reproduction; Anti-inflammatory activity; Cows; Luteinization; Ovulation; Prostaglandins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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100 1 $aMORAES, F. P. de
245 $aProstaglandin F2a regulation and function during ovulation and luteinization in cows.$h[electronic resource]
260 $c2021
520 $aAlthough prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF group (P < 0.05), and no effect of the treatments on intrafollicular P4 and E2 concentrations 24 h after GnRH. The prostaglandin metabolite (PGFM) concentrations in the FF were greater in cows receiving systemic PGF (P < 0.001), which prompted us to further check its role on ovulation. Therefore, in Experiment 3, in a final attempt to demonstrate the local effect of PGF on ovulation, cows with preovulatory follicles received an intrafollicular injection (IFI) of PBS (Control) or 100 ng/mL purified PGF (PGF group). PGF treatment did not affect the time of ovulation after IFI (66 ± 6.4 and 63 ± 8.5 h for control and PGF, respectively; P > 0.05), further suggesting that it has no direct effect in the ovulatory process. Based on our findings, we concluded that FM decreased PGF synthesis within the follicle, whereas PGF treatment decreased follicular vascularization. In addition, the in vivo model of intrafollicular injection evidenced that PGF alone is not able to locally induce ovulation.
650 $aAnimal reproduction
650 $aAnti-inflammatory activity
650 $aCows
650 $aLuteinization
650 $aOvulation
650 $aProstaglandins
650 $aOvulação
650 $aProstaglandina
650 $aReprodução Animal
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653 $aAção anti-inflamatória
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700 1 $aCASTRO, N. A. de
700 1 $aVIEIRA, A. D.
700 1 $aSCHNEIDER, A.
700 1 $aPFEIFER, L. F. M.
700 1 $aPEGORARO, L. M. C.
700 1 $aFERREIRA, R.
700 1 $aFERST, J. G.
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773 $tTheriogenology$gv. 171, p. 30-37, Sept. 2021.
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