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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
16/11/2018 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
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Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
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Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
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DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
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Palavras-Chave: |
CAZy; Metasecretome. |
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Thesaurus Nal: |
Lignocellulose. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
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Marc: |
LEADER 03644naa a2200301 a 4500
001 2099501
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI
100 1 $aALESSI, A. M.
245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource]
260 $c2018
520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation.
650 $aLignocellulose
653 $aCAZy
653 $aMetasecretome
700 1 $aBIRD, S. M.
700 1 $aOATES, N. C.
700 1 $aLI, Y.
700 1 $aDOWLE, A. A.
700 1 $aNOVOTNY, E. H.
700 1 $aAZEVEDO, E. R. de
700 1 $aBENNETT, J. P.
700 1 $aPOLIKARPOV, I.
700 1 $aYOUNG, J. P. W.
700 1 $aMCQUEEN-MASON, S. J.
700 1 $aBRUCE, N. C.
773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
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Registro original: |
Embrapa Solos (CNPS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Meio-Norte; Embrapa Rondônia. |
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Data corrente: |
30/09/2009 |
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Data da última atualização: |
15/04/2021 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
VILARINHO, A. A.; LOPES, A. de M.; FREIRE FILHO, F. R.; GONÇALVES, J. R. P.; ALVES, J. M. A.; MARINHO, J. T. de S.; VIEIRA JÚNIOR, J. R.; CAVALCANTE, E. da S. |
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Afiliação: |
Aloisio Alcantara Vilarinho, Embrapa Roraima; Altevir de Matos Lopes, Embrapa Amazônia Oriental; Francisco Rodrigues Freire Filho, Embrapa Meio-Norte; José Roberto Pupo Gonçalves, Embrapa Amazônia Ocidental; José Maria Arcanjo Alves, Universidade Federal de Roraima; José Tadeu de Souza Marinho, Embrapa Acre; José Roberto Vieira Júnior, Embrapa Rondônia; Emanuel da Silva Cavalcante, Embrapa Amapá. |
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Título: |
Melhoramento. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: ZILLI, J. E.; VILARINHO, A. A.; ALVES, J. M. A. (Ed.). A cultura do feijão-caupi na Amazônia brasileira. Boa Vista: Embrapa Roraima, 2009. p. 105-130. |
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Idioma: |
Português |
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Conteúdo: |
Melhoramento genético. Cultivares desenvolvidas para a Região Amazônica. Ipean V-69. Manaus. BR 8 Caldeirão. BR 4 Rio Branco e BR 5 Cana Verde. BR 2 Bragança e BR 3 Tracuateua. Amapá. BRS Mazagão. Cultivar BR 3 Tracuateua purificada. BRS Urubuquara e BRS Milênio. BRS Novaera. BRS Cauamé, BRS Tumucumaque, BRS Xiquexique e BRS Potengi. Cultivares utilizadas na Região Amazônica. |
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Palavras-Chave: |
Aspectos socioeconômicos; Caupi; Feijão-caupi; Histórico; Melhoramento genético; Vigna unguiculata (L) Walp. |
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Thesagro: |
Feijão de corda; Genética vegetal; Melhoramento; Melhoramento genético vegetal; Pesquisa; Produtividade; Variedade; Vigna Unguiculata. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
--
G Melhoramento Genético
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 01520naa a2200385 a 4500
001 1710786
005 2021-04-15
008 2009 bl uuuu u00u1 u #d
100 1 $aVILARINHO, A. A.
245 $aMelhoramento.
260 $c2009
520 $aMelhoramento genético. Cultivares desenvolvidas para a Região Amazônica. Ipean V-69. Manaus. BR 8 Caldeirão. BR 4 Rio Branco e BR 5 Cana Verde. BR 2 Bragança e BR 3 Tracuateua. Amapá. BRS Mazagão. Cultivar BR 3 Tracuateua purificada. BRS Urubuquara e BRS Milênio. BRS Novaera. BRS Cauamé, BRS Tumucumaque, BRS Xiquexique e BRS Potengi. Cultivares utilizadas na Região Amazônica.
650 $aAmazonia
650 $aFeijão de corda
650 $aGenética vegetal
650 $aMelhoramento
650 $aMelhoramento genético vegetal
650 $aPesquisa
650 $aProdutividade
650 $aVariedade
650 $aVigna Unguiculata
653 $aAspectos socioeconômicos
653 $aCaupi
653 $aFeijão-caupi
653 $aHistórico
653 $aMelhoramento genético
653 $aVigna unguiculata (L) Walp
700 1 $aLOPES, A. de M.
700 1 $aFREIRE FILHO, F. R.
700 1 $aGONÇALVES, J. R. P.
700 1 $aALVES, J. M. A.
700 1 $aMARINHO, J. T. de S.
700 1 $aVIEIRA JÚNIOR, J. R.
700 1 $aCAVALCANTE, E. da S.
773 $tIn: ZILLI, J. E.; VILARINHO, A. A.; ALVES, J. M. A. (Ed.). A cultura do feijão-caupi na Amazônia brasileira. Boa Vista: Embrapa Roraima, 2009. p. 105-130.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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