Data/hora: 11/06/2024 06:01:51

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 15/02/2024
Data da última atualização: | 15/02/2024
Autoria: | BATISTA, R. I. T. P.; DIAS, J. H.; GONÇALVES, J. D.; VERGANI, G. B.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da.
Afiliação: | RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; JENNIFFER HAUSCHILDT DIAS, Universidade Federal de Viçosa; JOEDSON DANTAS GONÇALVES, Universidade Estadual Paulista; GABRIEL BRUN VERGANI, Universidade Estadual Paulista; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense; JEFERSON FERREIRA DA FONSECA, CNPC.
Título: | The presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile.
Ano de publicação: | 2024
Fonte/Imprenta: | Theriogenology, v. 218, p. 208-213, Feb. 2024.
DOI: | 10.1016/j.theriogenology.2024.01.038.
Idioma: | Inglês
Conteúdo: | Abstract: Non-surgical embryo recovery (NSER) is usually preceded by a cervical relaxation in ovine donors, based on estradiol benzoate (EB), prostaglandin (PGF), and oxytocin (OT). However, it is hypothesized that, due to poorly understood mechanisms, EB can result in embryotoxic actions. To evaluate this, 20 min before NSER superovulated sheep were induced to cervical relaxation with 0.0 (G0.0), 0.5 (G0.5), or 1.0 mg (G1.0) of EB associated with 37.5 μg of PGF 16 h before NSER and 50 IU of OT. In doing so, the efficiency and duration of the NSER procedure showed no compromise (P > 0.05). Additionally, the presence of EB did not affect (P > 0.05) the embryo's morphological quality, the development dynamics, or the abundance of transcripts associated with embryonic quality (OCT4 and NANOG), cellular stress (HSP90 and PRDX1), and apoptosis (BCL2 and BAX). A similar result (P > 0.05) was also observed when comparing embryonic cryosurvival at 24 (52.0, 52.0, and 54.0) and 48 h (60.0, 54.0, and 58.0) of in vitro culture (G0.0, G0.5, and G1.0, respectively). Thus, we can conclude that EB use does not compromise embryonic quality and cryoresistance.
Palavras-Chave: | Cervical dilation; Dosage effects; Drug effects; Embryo survival; Expressão gênica; Gene expression profiling; NSER; Sobrevivência embrionária.
Thesagro: | Criopreservação; Estradiol; Genética Animal; Genética Molecular; Ovelha; Ovino; Progesterona; Reprodução; Transferência de Embrião.
Thesaurus Nal: | Animal genetics; Cryopreservation; Embryo transfer; Ewes; Females; Gene expression; Molecular genetics; Oxytocin; Prostaglandins; Reproduction; Sheep.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (40763-1) - UPC - AP - DD -  -  -  - DIS