Data/hora: 24/09/2024 05:50:21

Biblioteca(s): 	Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido.	
Data corrente: 	26/05/2023	
Data da última atualização: 	29/05/2023	
Autoria: 	FONSECA, F. C. de A.; ANTONINO, J. D.; MOURA, S. M. de; RODRIGUES-SILVA, P. L.; MACEDO, L. L. P. de; GOMES JÚNIOR, J. E.; LOURENCO, I. T.; LUCENA, W. A.; MORGANTE, C. V.; RIBEIRO, T. P.; MONNERAT, R. G.; RODRIGUES, M. A.; CUCCOVIA, I. M.; SILVA, M. C. M. da; SA, M. F. G. de.	
Afiliação: 	FERNANDO CAMPOS DE ASSIS FONSECA, University of Brasília; JOSÉ DIJAIR ANTONINO, University of Brasília; STÉFANIE MENEZES DE MOURA, National Institute of Science and Technology; PAOLO LUCAS RODRIGUES-SILVA, National Institute of Science and Technology; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; JOSÉ EDÍLSON GOMES JÚNIOR, Federal University of Brasília; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; WAGNER ALEXANDRE LUCENA, ARIG; CAROLINA VIANNA MORGANTE, CPATSA; THUANNE PIRES RIBEIRO, National Institute of Science and Technology; ROSE GOMES MONNERAT; MAGALI APARECIDA RODRIGUES, 7University of São Paulo; IOLANDA MIDEA CUCCOVIA, University of São Paulo; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen.	
Título: 	In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Bulletin of Entomological Research, v. 113, n. 3, p. 335-346, 2023.	
DOI: 	https://doi.org/10.1017/S000748532200061X	
Idioma: 	Inglês	
Notas: 	Na publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.	
Conteúdo: 	The sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields.	
Palavras-Chave: 	Aminopeptidase N; Broca gigante da cana-de-açúcar; Cry toxins; Modelagem molecular; Molecular modeling; Techin licus licus; Toxinas Cry.	
Thesagro: 	Bacillus Thuringiensis; Cana de Açúcar; Inseto; Praga; Saccharum Officinarum.	
Thesaurus Nal: 	Sugarcane.	
Categoria do assunto: 	--
O Insetos e Entomologia	
Registro original: 	Embrapa Semiárido (CPATSA)	
Exemplares: 	CENARGEN - (39165-1) - UPC - AP - DD -  -  -  - DIS
CPATSA - (60428-1) - UPC - AP - DD -  -  -  - DIS