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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
12/05/2005 |
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Data da última atualização: |
11/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA-SIQUEIRA, T. C. G.; OLIVEIRA, M. C. de S.; ARAÚJO JR., J. P.; AMARANTE, A. F. T. |
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Afiliação: |
T. C. G. OLIVEIRA-SEQUEIRA, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; J. P. ARAÚJO JR., UNESP; A. F. T. AMARANTE, UNESP. |
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Título: |
PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
International Journal of Parasitology, v. 35, p. 105-111, 2005. |
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DOI: |
10.1016/j.ijpara.2004.09.002 |
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Idioma: |
Inglês |
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Conteúdo: |
PCR and nested-PCR methods were used to assess the frequency of Babesia bovis and Babesia bigemina infection in Boophilus microplus engorged females and eggs and in cattle reared in an area with endemic babesiosis. Blood and the engorged female ticks were from 27 naturally infested calves and 25 crossbred cows. The frequency of both Babesia species was similar in calves and cows (P>0.05). Babesia bovis was detected in 23 (85.2%) calves and in 25 (100%) cows and B. bigemina was detected in 25 (92.6%) calves and in 21 (84%) cows. Mixed infections with the both Babesia species were identified in 42 animals, 21 in each age category. Of female ticks engorged on calves, 34.9% were negative and single species infection with B. bigemina (56.2%) was significantly more frequent (P0.05) to the frequency of single B. bigemina infection (15.9%). Mixed Babesia infection was lower (PMenosPCR and nested-PCR methods were used to assess the frequency of Babesia bovis and Babesia bigemina infection in Boophilus microplus engorged females and eggs and in cattle reared in an area with endemic babesiosis. Blood and the engorged female ticks were from 27 naturally infested calves and 25 crossbred cows. The frequency of both Babesia species was similar in calves and cows (P>0.05). Babesia bovis was detected in 23 (85.2%) calves and in 25 (100%) cows and B. bigemina was detected in 25 (92.6%) calves and in 21 (84%) cows. Mixed infections with the both Babesia species were identified in 42 animals, 21 in each age category. Of female ticks engorged on calves, 34.9% were negative and single species infection with B. bigemina (56.2%) was significantly more frequent (P0.05) to the frequency of single B. bigemina infection (15.9%). Mixed Babesia infection was lower (PMostrar Tudo |
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Palavras-Chave: |
Chain reaction; Polymerase. |
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Thesagro: |
Babesia Bigemina; Babesia Bovis; Boophilus Microplus. |
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Thesaurus Nal: |
Brazil. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/15589/1/PROCIMCSO2005.00002.pdf
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Marc: |
LEADER 02474naa a2200241 a 4500
001 1047048
005 2023-05-11
008 2005 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ijpara.2004.09.002$2DOI
100 1 $aOLIVEIRA-SIQUEIRA, T. C. G.
245 $aPCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle.$h[electronic resource]
260 $c2005
520 $aPCR and nested-PCR methods were used to assess the frequency of Babesia bovis and Babesia bigemina infection in Boophilus microplus engorged females and eggs and in cattle reared in an area with endemic babesiosis. Blood and the engorged female ticks were from 27 naturally infested calves and 25 crossbred cows. The frequency of both Babesia species was similar in calves and cows (P>0.05). Babesia bovis was detected in 23 (85.2%) calves and in 25 (100%) cows and B. bigemina was detected in 25 (92.6%) calves and in 21 (84%) cows. Mixed infections with the both Babesia species were identified in 42 animals, 21 in each age category. Of female ticks engorged on calves, 34.9% were negative and single species infection with B. bigemina (56.2%) was significantly more frequent (P<O.OI) than with B. bovis (4.7%). Most of the females (60.8%) engorged on cows did not show Babesia spp. infection and the frequency of single B. bovis infection (17.6%) was similar (P>0.05) to the frequency of single B. bigemina infection (15.9%). Mixed Babesia infection was lower (P<O.OI) than single species infection in female ticks engorged either in cows (5.7%) or in calves (4.3%). An egg sample from each female was analysed for the presence of Babesia species. Of the egg samples from female ticks infected with B. bovis, 26 (47.3%) were infected while from those from female ticks infected with B. bigemina 141 (76.6%) were infected (P<0.01). The results showed that although the frequency of both species of Babesia was similar in calves and cows, the infectivity of B. bigemina was higher to ticks fed on calves while to those ticks fed on cows the infectivity of both Babesia species was similar.
650 $aBrazil
650 $aBabesia Bigemina
650 $aBabesia Bovis
650 $aBoophilus Microplus
653 $aChain reaction
653 $aPolymerase
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aARAÚJO JR., J. P.
700 1 $aAMARANTE, A. F. T.
773 $tInternational Journal of Parasitology$gv. 35, p. 105-111, 2005.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
