Data/hora: 23/06/2024 20:28:35

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 15/11/2017
Data da última atualização: | 23/11/2017
Autoria: | GOMES, S. V. F.; PORTUGAL, L. A.; ANJOS, J. P. dos; JESUS, O. N. de; OLIVEIRA, E. J. de; DAVID, J. P.; DAVID, J. M.
Afiliação: | SILVANA V. F. GOMES, UFBA; LINDOMAR A. PORTUGAL, UFBA; JEANCARLO P. DOS ANJOS, UFBA; ONILDO NUNES DE JESUS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF; JUCENI P. DAVID, UFBA; JORGE M. DAVID, UFBA.
Título: | Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species.
Ano de publicação: | 2017
Fonte/Imprenta: | Microchemical Journal, V.132, P.28-35, 2017.
ISSN: | 0026-265X
Idioma: | Inglês
Conteúdo: | This work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively.
Thesagro: | Maracujá.
Thesaurus Nal: | Passiflora.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (32028-1) - UPC - AP - DD - Publicação digital -  -  - DIS