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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
13/03/2017 |
Data da última atualização: |
13/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BONDAN, C.; ZANELLA, E.; ZANELLA, R.; POETINI, M. R.; MARQUES, M. G.; SOARES, J. C. M. |
Afiliação: |
CARLOS BONDAN, UPF/FAMV; ERALDO ZANELLA, UPF/FAMV; RICARDO ZANELLA, UPF/FAMV; MÁRCIA RÓSULA POETINI, Unipampa/Caçapava do Sul; MARIANA GROKE MARQUES, CNPSA; JULIO C.M. SOARES, Unipampa/Caçapava do Sul. |
Título: |
xidative status of boar semen during storage. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
American Journal of Biochemistry and Biotechnology, Al Ain, v. 12, n. 2, p. 95-101, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 ?M. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen. MenosAbstract: The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 ?M. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In ... Mostrar Tudo |
Palavras-Chave: |
ROS; Trolox. |
Thesagro: |
Armazenamento; Coleta de semen; Inseminação artificial; Reprodução animal; Suíno. |
Thesaurus Nal: |
Animal reproduction; Artificial insemination; Swine. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02558naa a2200301 a 4500 001 2066914 005 2017-03-13 008 2016 bl uuuu u00u1 u #d 100 1 $aBONDAN, C. 245 $axidative status of boar semen during storage.$h[electronic resource] 260 $c2016 520 $aAbstract: The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 ?M. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen. 650 $aAnimal reproduction 650 $aArtificial insemination 650 $aSwine 650 $aArmazenamento 650 $aColeta de semen 650 $aInseminação artificial 650 $aReprodução animal 650 $aSuíno 653 $aROS 653 $aTrolox 700 1 $aZANELLA, E. 700 1 $aZANELLA, R. 700 1 $aPOETINI, M. R. 700 1 $aMARQUES, M. G. 700 1 $aSOARES, J. C. M. 773 $tAmerican Journal of Biochemistry and Biotechnology, Al Ain$gv. 12, n. 2, p. 95-101, 2016.
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Embrapa Suínos e Aves (CNPSA) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
23/05/1997 |
Data da última atualização: |
25/01/2012 |
Autoria: |
GOES, M. de; MENDES, R. A. |
Afiliação: |
EMBRAPA-CENARGEN. |
Título: |
Conservacao de germoplasma vegetal in vitro criopreservacao. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
In: CURSO DE CONSERVACAO DE GERMOPLASMA SEMENTE. Brasilia: [s.n.], 1994. 10p. |
Idioma: |
Português |
Palavras-Chave: |
Germoplasma vegetal; In-vitro; Plant; Vegetal germplasm. |
Thesagro: |
Criopreservação; Germoplasma; Planta. |
Thesaurus NAL: |
cryopreservation; germplasm. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00618nam a2200217 a 4500 001 1171303 005 2012-01-25 008 1994 bl uuuu u00u1 u #d 100 1 $aGOES, M. de 245 $aConservacao de germoplasma vegetal in vitro criopreservacao. 260 $aIn: CURSO DE CONSERVACAO DE GERMOPLASMA SEMENTE. Brasilia: [s.n.], 1994. 10p.$c1994 650 $acryopreservation 650 $agermplasm 650 $aCriopreservação 650 $aGermoplasma 650 $aPlanta 653 $aGermoplasma vegetal 653 $aIn-vitro 653 $aPlant 653 $aVegetal germplasm 700 1 $aMENDES, R. A.
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