|
|
Registros recuperados : 7 | |
2. | | MICLO, L.; GIRARDET, J. -M.; EGITO, A. S. do; MOLLÉ, D.; MARTIN, P.; GAILLARD, J. L. The primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk. Proteomics, v. 7, n. 8, p. 1327-1335, Mar. 2007. Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
3. | | EGITO, A. S. do; MICLO, L.; HAUSSARD, M.; POIRSON, C.; GIRARDET, J. M.; GAILLARD, J. L. Identificação de peptídeos de caseínas do leite de égua com atividade anti-hipertensiva. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 29., 2002, Gramado, RS. Saúde ambiental, animal e humana: uma questão de sobrevivência: anais. Gramado: Sociedade Brasileira de Medicina Veterinária, 2002. 1 CD ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
5. | | GIRARDET, J.-M.; SAULNIER, F.; DRIOU, A.; LINDEN, G.; CODDEVILLE, B.; SPIK, G. High performance electrophoresis chromatography of bovine milk component 3 glycoproteins. Journal of Dairy Science, v. 77, n. 5, p. 1205–1215, 1994. Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
6. | | GIRARDET, J. -M.; N'NEGUE, M. -A.; EGITO, A. S. do; CAMPAGNA, S.; LAGRANGE, A.; GAILLARD, J. -L. Multiple forms of equine a-lactalbumin: evidence for N-glycosylated and deamidated forms. International Dairy Journal, v. 14, n. 3, p. 207-217, Mar., 2004. Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
7. | | EGITO, A. S. do; GIRARDET, J. -M.; LAGUNA, L. E.; POIRSON, C.; MOLLÉ, D.; MICLO, L.; HUMBERT, G.; GAILLARD, J. -L. Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein. International Dairy Journal, v. 17, n. 7, p. 816-825, Jul. 2007. Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
Registros recuperados : 7 | |
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
29/02/2008 |
Data da última atualização: |
25/09/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
MICLO, L.; GIRARDET, J. -M.; EGITO, A. S. do; MOLLÉ, D.; MARTIN, P.; GAILLARD, J. L. |
Afiliação: |
Miclo L, Unité de Recherche sur l'Animal et les Fonctionnalités des Produits Animaux (UR AFPA L'Institut National de la Recherche Agronomique (INRA); Girardet J. M., Unité de Recherche sur l'Animal et les Fonctionnalités des Produits Animaux (UR AFPA L'Institut National de la Recherche Agronomique (INRA); ANTONIO SILVIO DO EGITO, CNPC; Mollé D, L'Institut National de la Recherche Agronomique; Martin P., Unité Génomique et Physiologie de la Lactation (GLP) Institut National de la Recherche Agronomique; Gaillard J. L., Unité de Recherche sur l'Animal et les Fonctionnalités des Produits Animaux (UR AFPA L'Institut National de la Recherche Agronomique (INRA). |
Título: |
The primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Proteomics, v. 7, n. 8, p. 1327-1335, Mar. 2007. |
Idioma: |
Inglês |
Conteúdo: |
Highly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10 591 ± 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine -casein (226 residues). This low-Mr variant of equine -casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine -casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups. |
Palavras-Chave: |
Equine Milk; Fosforização; High Pressure Liquid; Leite de égua. |
Thesagro: |
Caseína; Enzima. |
Thesaurus NAL: |
Alternative splicing; Amino acid sequences; Beta-casein; Chromatography; Food chemistry; Gel electrophoresis; Horses; Mass spectrometry; Peptides; Phosphorylation; Protein isoforms. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02070naa a2200385 a 4500 001 1521814 005 2019-09-25 008 2007 bl uuuu u00u1 u #d 100 1 $aMICLO, L. 245 $aThe primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk.$h[electronic resource] 260 $c2007 520 $aHighly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10 591 ± 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine -casein (226 residues). This low-Mr variant of equine -casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine -casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups. 650 $aAlternative splicing 650 $aAmino acid sequences 650 $aBeta-casein 650 $aChromatography 650 $aFood chemistry 650 $aGel electrophoresis 650 $aHorses 650 $aMass spectrometry 650 $aPeptides 650 $aPhosphorylation 650 $aProtein isoforms 650 $aCaseína 650 $aEnzima 653 $aEquine Milk 653 $aFosforização 653 $aHigh Pressure Liquid 653 $aLeite de égua 700 1 $aGIRARDET, J. -M. 700 1 $aEGITO, A. S. do 700 1 $aMOLLÉ, D. 700 1 $aMARTIN, P. 700 1 $aGAILLARD, J. L. 773 $tProteomics$gv. 7, n. 8, p. 1327-1335, Mar. 2007.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|