Data/hora: 17/06/2024 10:55:53

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	05/07/2013	
Data da última atualização: 	19/05/2023	
Autoria: 	BRITTO, D. S.; PIROVANI, C. P.; ANDRADE, B. S.; SANTOS, T. P. dos; PUNGARTNIK, C.; CASCARDO, J. C. M.; MICHELI, F.; GESTEIRA, A. da S.	
Afiliação: 	DAHYANA SANTOS BRITTO, UESC; CARLOS PRIMINHO PIROVANI, UESC; BRUNO SILVA ANDRADE, UESB; TASSIARA PEREIRA DOS SANTOS, UESC; CRISTINA PUNGARTNIK, UESC; JÚLIO CEZAR M. CASCARDO, UESC; FABIENNE MICHELI, CIRAD; ABELMON DA SILVA GESTEIRA, CNPMF.	
Título: 	Recombinant b-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Molecular Biology Reports, 2013.	
ISSN: 	0301-4851	
DOI: 	10.1007/s11033-013-2640-1	
Idioma: 	Inglês	
Conteúdo: 	In this work, we identified a gene from Theobroma cacao L. genome and cDNA libraries, named TcGlu2, that encodes a b-1,3-1,4-glucanase. The TcGlu2 ORF was 720 bp in length and encoded a polypeptide of 239 amino acids with a molecular mass of 25.58 kDa. TcGlu2 contains a conserved domain characteristic of b- 1,3-1,4-glucanases and presented high protein identity with b-1,3-1,4-glucanases from other plant species. Molecular modeling of TcGlu2 showed an active site of 13 amino acids typical of glucanase with b-1,3 and 1,4 action mode. The recombinant cDNA TcGlu2 obtained by heterologous expression in Escherichia coli and whose sequence was confirmed by mass spectrometry, has a molecular mass of about 22 kDa (with His-Tag) and showed antifungal activity against the fungus Moniliophthora perniciosa, causal agent of the witches? broom disease in cacao. The integrity of the hyphae membranes of M. perniciosa, incubated with protein TcGlu2, was analyzed with propidium iodide. After 1 h of incubation, a strong fluorescence emitted by the hyphae indicating the hydrolysis of the membrane by TcGlu2, was observed. To our knowledge, this is the first study of a cacao b-1,3-1,4-glucanase expression in heterologous system and the first analysis showing the antifungal activity of a b-1,3-1,4-glucanase, in particular against M. perniciosa.	
Thesagro: 	Cacau.	
Thesaurus Nal: 	Antifungal proteins; Moniliophthora perniciosa.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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