Data/hora: 02/06/2024 14:41:17

Biblioteca(s): | Embrapa Cerrados.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 16/12/2013
Data da última atualização: | 10/12/2014
Autoria: | PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C.
Afiliação: | ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP.
Título: | Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species.
Ano de publicação: | 2013
Fonte/Imprenta: | Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013.
ISSN: | 0003-4746
DOI: | 10.1111/aab.12028
Idioma: | Inglês
Conteúdo: | One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n&#8201;=&#8201;18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87&#8201;bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score&#8201;&#8805;&#8201;5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0&#8201;cM, with an average distance between adjacent markers of 3.8&#8201;cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping.
Thesagro: | Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético.
Thesaurus Nal: | Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Cerrados (CPAC)
Exemplares: | CPAC - (33762-1) - UPC - AP - PP - S1633 -  - S1633 - DIS