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Registros recuperados : 149 | |
81. | | SOUZA, T. C. G. D. de; GAVA, D.; LEME, R. A.; SCHAEFER, R.; PORTO, G. S.; ALFIERI, A. A. Porcine circovirus type 3 in free-living wild boars in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 757. Biblioteca(s): Embrapa Suínos e Aves. |
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82. | | RECH, R. R.; GAVA, D.; SILVA, M. C. da; FERNANDES, L. T.; HAACH, V.; ZANELLA, J. R. C.; SCHAEFER, R. Porcine respiratory disease complex after the introduction of H1N1/2009 influenza virus in Brazil. Zoonoses and Public Health, v. 65, n.1, p. e155-e161, 2018. Biblioteca(s): Embrapa Suínos e Aves. |
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83. | | SILVA, M. C.; RECH, R. R.; GAVA, D.; KUCHIISHI, S. S.; SCHAEFER, R.; LANGOHR, I. M.; ZANELLA, J. R. C. Porcine respiratory disease complex in southern Brazil: pathogens and associated histopathology. In: CONCURRENT ANNUAL METING, 2013, Montreál. Abstract detail. Montreál: American College of Veterinary Pathologists, 2013. Poster D-32. Biblioteca(s): Embrapa Suínos e Aves. |
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84. | | SCHAEFER, R.; GAVA, D.; CANTAO, M. E.; SERÃO, V. H. B.; SILVA, M. C.; MORES, N.; ZANELLA, J. R. C. PCV2 disease in vaccinated growing pigs in Southern Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 23., Cancun. Proceedings... León, México: IPVS, 2014. p. 522 Biblioteca(s): Embrapa Suínos e Aves. |
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85. | | ZANELLA, J. R. C.; GAVA, D.; SCHAEFER, R.; COLDEBELLA, A.; SILVEIRA, S.; SIMON, N. L.; SCHIOCHET, M. F.; SCHWARZ, P. Perfil da infecção por vírus de influenza em suínos no Brasil após a introdução do vírus pandêmico H1N1 em 2009. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 16., 2013, Cuiabá. Anais... Guiabá: Abraves; UFMT, 2013. 1 CD-ROM. Biblioteca(s): Embrapa Suínos e Aves. |
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86. | | GAVA, D.; SCHAEFER, R.; SERRÃO, V. H. B.; CANTAO, M. E.; SILVA, M. C.; ZANELLA, J. R. C. Mutations in the capsid protein of porcine circovirus type 2 indicates a viral immune evasion. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 19, p. 234-235, 2014. Suppl. 2. Edição dos anais do 25º. Brazilian Congress of Virology e 9º. Mercosur Meeting of Virology, Ribeirão Preto, set./out, 2014. Biblioteca(s): Embrapa Suínos e Aves. |
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87. | | MORES, N.; CARON, L.; MORES, M. A. Z.; GAVA, D.; ESTEVES, P. A.; ZANELLA, J. R. C. Mumificação fecal associado ao circovírus suíno tipo 2 (PCV2) em um rebanho livre de patógenos específicos (SPF). In: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 7., Porto Alegre, RS. Anais... Porto Alegre: UFRGS, 2012. p. 283-286. Projeto: 03.07.05.019. SINSUI. Biblioteca(s): Embrapa Suínos e Aves. |
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88. | | MORI, A. P.; PERETTI, R. C.; FERRAZ, R.; FERRAZ, G. M.; GAVA, D.; HAACH, V.; SCHAEFER, R. Occurrence of influenza A virus subtypes in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 773. Biblioteca(s): Embrapa Suínos e Aves. |
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89. | | MORI, A. P.; PERETTI, R. C.; FERRAZ, R.; FERRAZ, G. M.; GAVA, D.; HAACH, V.; SCHAEFER, R. Occurrence of influenza A virus subtypes in Brazil. In: ALLEN D. LEMAN SWINE CONFERENCE, 2020, Saint Paul. Research Abstract. Saint Paul: University of Minnesota/College of Veterinary Medicine, 2020. Poster number: 70. Biblioteca(s): Embrapa Suínos e Aves. |
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90. | | GAVA, D.; SERRÃO, V. H. B.; FERNANDES, L. T.; CANTAO, M. E.; ZANELLA, J. R. C.; MORES, N.; SCHAEFER, R. Structure analysis of capsid protein of porcine circovirus type 2 from pigs with systemic disease. Brazilian Journal of Microbiology, v. 49, p. 351-357, 2018. Biblioteca(s): Embrapa Suínos e Aves. |
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91. | | GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. Journal of Virological Methods, v. 219, p. 14-17, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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92. | | MACIAG, S. S.; BELLAVER, F. A. V.; FONSECA, F. M. da; BOMBASSARO, G. E.; GAVA, D.; LOPES, L. dos S.; BASTOS, A. P. A. Comparison of cell viability in fresh and frozen porcine colostrums. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 561. Biblioteca(s): Embrapa Suínos e Aves. |
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93. | | BORDIN, L. C.; GAVA, D.; SIMON, N. L.; ROCHA, C. S.; ESTEVES, P. A.; MORES, N.; COLDEBELLA, A.; ZANELLA, J. R. C.; VAZ, E. K. Comparison of conventional and real time PCR to detect Mycoplasma suis in Brazilian herds with reproductive disorders. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 22., 2012, Jeju. Proceedings... Jeju: IPVS, 2012. p. 689. Projeto/Plano de Ação: 02.11.01.006. Biblioteca(s): Embrapa Suínos e Aves. |
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94. | | BOMBASSARO, G. E.; BELLAVER, F. A. V.; FONSECA, F. M. da; MACIAG, S. S.; GAVA, D.; LOPES, L. dos S.; BASTOS, A. P. A. Comparison of immune cells of colostrum from gilts and sows. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 562. Biblioteca(s): Embrapa Suínos e Aves. |
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95. | | HAACH, V.; BASTOS, A. P. A.; GAVA, D.; FONSECA, F. M. da; MORES, M. A. Z.; COLDEBELLA, A.; FRANCO, A. C.; SCHAEFER, R. Cellular and humoral immunity elicited by an Influenza A polyvalent virosomal vaccine in pigs. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 421. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 421. Biblioteca(s): Embrapa Suínos e Aves. |
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96. | | TOCHETTO, C.; JUNQUEIRA, D. M.; ANDERSON, T. K.; GAVA, D.; HAACH, V.; CANTAO, M. E.; BAKER, A. L. V.; SCHAEFER, R. Introductions of human-origin Seasonal H3N2, H1N2 and pre-2009 H1N1 Influenza Viruses to swine in Brazil. Viruses, v. 15, n. 576, 2023. Biblioteca(s): Embrapa Suínos e Aves. |
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97. | | ZANELLA, J. R. C.; GAVA, D.; ZANELLA, E. L.; BASSI, S. S.; ASCOLI, K.; SIMON, N. L.; DAHMER, A.; MORÉS, N. Investigation of tropism and reproductive changes in boar naturally infected with porcine circovirus 2 (PCV2). In: INTERNATIONAL SYMPOSIUM ON EMERGING AND RE-EMERGING PIG DISEASES, 5., 2007, Krakow. Proceedings... Krakow: [s.n.], 2007. p. 95 Projeto: 03.04.33.800 Biblioteca(s): Embrapa Suínos e Aves. |
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98. | | BORDIN, L. C.; GAVA, D.; SONÁLIO, K.; DREIBI, M. L. M.; ZANELLA, J. R. C.; MORÉS, N.; OLIVEIRA, L. G. de; VAZ, E. K. Investigation of hemotropic Mycoplasmas in fetuses and sows with reproductive failure. Veterinary and Animal Science, v. 12, n.100175, 2021. Biblioteca(s): Embrapa Suínos e Aves. |
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99. | | REBELATTO, R.; KLEIN, C. S.; BELLAVER, F. A. V.; LOCATELLI, C.; GAVA, D.; KUCHIISHI, S. S.; CARON, L.; SILVA, V. S. Isolados bacterianos provenientes de granjas de suínos e avaliação da sensibilidade a antimicrobianos. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 15., 2011, Fortaleza. Anais de palestras e resumos. Fortaleza: Abraves, 2011. 1 CD-ROM. Projeto: 03.09.00.046. Biblioteca(s): Embrapa Suínos e Aves. |
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100. | | SILVEIRA, S.; SCHIOCHET, M. F.; SIMON, N. L.; RTTERBUSCH, G. A.; GAVA, D.; ROCHA, C. S.; CARON, L.; SCHAEFER, R.; CIACCI. Isolamento do vírus Influenza A em pulmões de suínos. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA , 5., 2011, Concórdia. Resumos. Concórdia: Embrapa Suínos e Aves, 2011. p. 35. JINC. Projeto/Plano de Ação: 02.11.01.006. Biblioteca(s): Embrapa Suínos e Aves. |
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Registros recuperados : 149 | |
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Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
04/11/2019 |
Data da última atualização: |
04/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOUZA, A. R. de; YAMIN, M.; GAVA, D.; ZANELLA, J. R. C.; GATTI, M. S. V.; BONAFE, C. S. F.; LIMA NETO, D. F. de. |
Afiliação: |
ANCELMO RABELO DE SOUZA, UNICAMP; MARRIAM YAMIN, UNICAMP; DANIELLE GAVA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA; MARIA SILVIA VICCARI GATTI, UNICAMP; CARLOS FRANCISCO SAMPAIO BONAFE, UNICAMP; DANIEL FERREIRA DE LIMA NETO, UNICAMP. |
Título: |
Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Virology Journal, v. 16, art. 75, 2019. |
DOI: |
10.1186/s12985-019-1165-1 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized. |
Palavras-Chave: |
Epitope prediction; Porcine parvovirus; Spot synthesis. |
Thesagro: |
Parvovirose; Suinocultura; Virologia. |
Thesaurus NAL: |
Epitope mapping; Ungulate protoparvovirus 1. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204209/1/final9080.pdf
|
Marc: |
LEADER 02185naa a2200301 a 4500 001 2113864 005 2019-11-04 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1186/s12985-019-1165-1$2DOI 100 1 $aSOUZA, A. R. de 245 $aPorcine parvovirus VP1/VP2 on a time series epitope mapping$bexploring the effects of high hydrostatic pressure on the immune recognition of antigens.$h[electronic resource] 260 $c2019 520 $aAbstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized. 650 $aEpitope mapping 650 $aUngulate protoparvovirus 1 650 $aParvovirose 650 $aSuinocultura 650 $aVirologia 653 $aEpitope prediction 653 $aPorcine parvovirus 653 $aSpot synthesis 700 1 $aYAMIN, M. 700 1 $aGAVA, D. 700 1 $aZANELLA, J. R. C. 700 1 $aGATTI, M. S. V. 700 1 $aBONAFE, C. S. F. 700 1 $aLIMA NETO, D. F. de 773 $tVirology Journal$gv. 16, art. 75, 2019.
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