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Registros recuperados : 3 | |
1. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014. Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | COLLADO, M. del; SARAIVA, N. Z.; LOPES, F. L.; GASPAR, R. C.; PADILHA, L. C.; COSTA, R. R.; ROSSI, G. F.; VANTINI, R.; GARCIA, J. M. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016. Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ARNOLD, D. R.; CORRÊA, C. A. P.; LORENA, L. L. G.; GASPAR, R. C.; ROSSI, G. F.; IFRAM, A. M.; PENTEADO, J. C. T.; MINGOTI, G.; LOPES, F. L. Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro proeduced bovine embryos. Pesquisa Veterinária Brasileira, Rio de janeiro, v. 35, n. 7, p. 605-612. jul. 2015. Título em português: Suplementação com soro fetal bovino altera a modificação de histona H3R26me2 durante o período pré-implantacional em embriões bovinos produzidos in vitro. Biblioteca(s): Embrapa Unidades Centrais. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
Registros recuperados : 3 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
13/03/2017 |
Data da última atualização: |
19/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
COLLADO, M. del; SARAIVA, N. Z.; LOPES, F. L.; GASPAR, R. C.; PADILHA, L. C.; COSTA, R. R.; ROSSI, G. F.; VANTINI, R.; GARCIA, J. M. |
Afiliação: |
Maite del Collado, USP/UNESP; NAIARA ZOCCAL SARAIVA, CPATU; Flavia L. Lopes, UNESP; Roberta C. Gaspar, UNESP; Luciana C. Padilha, UNESP; Roberta R. Costa, USP; Guilherme F. Rossi, UNESP; Roberta Vantini, UNESP; Joaquim M. Garcia, UNESP. |
Título: |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016. |
DOI: |
http://dx.doi.org/10.1071/RD15067 |
Idioma: |
Inglês |
Conteúdo: |
Proper oocyte maturation is crucial for subsequent embryo development; however, oocyte mitochondrial and lipid-droplet behaviour are still poorly understood. Although excessive lipid accumulation during in vitro production (IVP) of bovine embryos has been linked with impaired cryotolerance, lipid oxidation is essential for adequate energy supply. Fetal bovine serum (FBS) and bovine serum albumin (BSA) are supplements used during IVP, containing high and low lipid content, respectively. This study aimed to understand how these supplements influence oocyte mitochondrial and lipid behaviour during in vitro maturation (IVM) in comparison to in vivo maturation, as well as their influence on development rates and embryo lipid accumulation during IVP. We demonstrate that only in vivo-matured oocytes maintained correlation between lipid content and active mitochondria. IVM media containing FBS increased total lipid content 18-fold and resulted in higher lipid accumulation in oocytes when compared with media with BSA. IVM using a lower FBS concentration combined with BSA resulted in satisfactory maturation and embryo development and also reduced lipid accumulation in blastocysts. In conclusion, IVM causes changes in mitochondrial and lipid dynamics, which may have negative effects on oocyte development rates and embryo lipid accumulation. Moreover, decreasing FBS concentrations during IVM may reduce embryo lipid accumulation without affecting production rates |
Thesagro: |
Bovino; Maturação; Reprodução. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02358naa a2200265 a 4500 001 2066875 005 2022-05-19 008 2016 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1071/RD15067$2DOI 100 1 $aCOLLADO, M. del 245 $aInfluence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos.$h[electronic resource] 260 $c2016 520 $aProper oocyte maturation is crucial for subsequent embryo development; however, oocyte mitochondrial and lipid-droplet behaviour are still poorly understood. Although excessive lipid accumulation during in vitro production (IVP) of bovine embryos has been linked with impaired cryotolerance, lipid oxidation is essential for adequate energy supply. Fetal bovine serum (FBS) and bovine serum albumin (BSA) are supplements used during IVP, containing high and low lipid content, respectively. This study aimed to understand how these supplements influence oocyte mitochondrial and lipid behaviour during in vitro maturation (IVM) in comparison to in vivo maturation, as well as their influence on development rates and embryo lipid accumulation during IVP. We demonstrate that only in vivo-matured oocytes maintained correlation between lipid content and active mitochondria. IVM media containing FBS increased total lipid content 18-fold and resulted in higher lipid accumulation in oocytes when compared with media with BSA. IVM using a lower FBS concentration combined with BSA resulted in satisfactory maturation and embryo development and also reduced lipid accumulation in blastocysts. In conclusion, IVM causes changes in mitochondrial and lipid dynamics, which may have negative effects on oocyte development rates and embryo lipid accumulation. Moreover, decreasing FBS concentrations during IVM may reduce embryo lipid accumulation without affecting production rates 650 $aBovino 650 $aMaturação 650 $aReprodução 700 1 $aSARAIVA, N. Z. 700 1 $aLOPES, F. L. 700 1 $aGASPAR, R. C. 700 1 $aPADILHA, L. C. 700 1 $aCOSTA, R. R. 700 1 $aROSSI, G. F. 700 1 $aVANTINI, R. 700 1 $aGARCIA, J. M. 773 $tReproduction, Fertility and Development$gv. 28, n. 11, p. 1721-1732, 2016.
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