Data/hora: 17/06/2024 08:11:22

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 10/01/2001
Data da última atualização: | 08/06/2018
Autoria: | FORATO, L. A.; COLNAGO, L. A.; GARRATT, R. C.; LOPES, M. A.
Afiliação: | EMBRAPA-CNPMS.
Título: | Identification of free fatty acids in maize protein bodies and purified a zeins by 13C and 1H nuclear magnetic resonance.
Ano de publicação: | 2000
Fonte/Imprenta: | Biochimica et Biophysica Acta, Amsterdam, v. 1543, p. 106-114, 2000.
Idioma: | Inglês
Conteúdo: | Zeins, the maize storage proteins, are the most abundant proteins in the corn endosperm, and are synthesized on the rough endoplasmatic reticulum and deposited in discrete organelles called protein bodies. Several authors, using circular dichroism and optical rotatory dispersion, have concluded that these proteins have a high a-helical content in alcoholic solution. In this work we have studied these proteins, within the protein bodies themselves and after extraction from the corn grains with 70% ethanol, using NMR (nuclear magnetic resonance) spectroscopy. We conclusively demonstrate the presence of free fatty acids within both the protein bodies and also in the alcohol extracted a zeins. We present evidence for a direct interaction between the free fatty acids and the a zein proteins within the protein body and suggest possible mechanisms by which such an association has arisen during the evolution of the maize endosperm.
Thesagro: | Biologia Molecular; Bioquímica; Metabolismo; Nitrogênio.
Thesaurus Nal: | biochemistry; metabolism; molecular biology; nitrogen.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (12829-1) - UPC - AP - DD -  -  -  - DIS