Data/hora: 20/06/2024 15:41:50

Biblioteca(s): 	Embrapa Cerrados.	
Data corrente: 	22/11/2021	
Data da última atualização: 	22/11/2021	
Autoria: 	SANTOS‑TIERNO, R.; GARCIA, R.; FONSECA, E.; FALEIRO, F. G.; MOREIRA, D.; PACHECO, G.; MANSUR, E.	
Afiliação: 	RAPHAELA SANTOS‑TIERNO; RENATA GARCIA; EDUARDO FONSECA; FABIO GELAPE FALEIRO, CPAC; DAVYSON MOREIRA; GEORGIA PACHECO; ELISABETH MANSUR.	
Título: 	Light quality and explant type modulate growth, antioxidant properties and bioactive compounds production of calluses of Passiflora setacea cv BRS Pérola do Cerrado.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Plant Cell, Tissue and Organ Culture, v. 147, p. 635-646, 2021.	
Idioma: 	Inglês	
Conteúdo: 	Passiflora species have high industrial and medicinal relevance, due to presence of specific metabolites and their recognized pharmacological activities. In vitro systems allow the modulation of secondary metabolites production according to culture conditions. In this work, we report the effect of different light emitting diodes (LED) on induction, metabolite production, and antioxidant properties of calluses derived from stem and leaf segments of P. setacea cv BRS Pérola do Cerrado. Friable calluses were induced on MSM medium supplemented with picloram, under different light qualities (white fluorescent lamps or different types of LEDs) or in the dark. Light quality contributed more significantly for biomass accumulation than the other variables. Callus formation was observed in response to all treatments, although the highest biomass accumulation was induced by red LED. Chromatographic analyses indicated that blue LED induced the highest production of bioactive substances. All samples displayed low antioxidant potential by the DPPH assay, but showed a high capacity to chelate iron. The activity of antioxidant enzymes was also evaluated. The incubation under red LED caused an increase on the activity of superoxide dismutase, catalase and ascorbate peroxidase in calluses derived from internodal segments induced under red LED. This is the first study evaluating the effect of lighting conditions in in vitro systems of P. setacea cv BRS Pérola do Cerrado, thus opening new possibilities of study and utilization.	
Palavras-Chave: 	Diodos emissores de luz; DPPH; Enzimas antioxidantes; Fitoquímica; Quelação de ferro.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Cerrados (CPAC)	
Exemplares: 	CPAC - (37156-1) - UPC - AP - DD - DIGITAL -  - DIGITAL - DIS